Pearl Lab

DNA Repair

Recognition, Repair and Signalling of DNA Damage
(CR-UK DNA Repair Enzymes Research Group)

SIngle Strand Break Repair

We are studying the structural basis for recognition and repair of DNA single-strand breaks and gaps by PARPs and the PARP1/XRCC1 SSBR system.
Ongoing projects include :
  • Structural Basis of gap and nick recognition by PARP1, PARP2 and PARP3
  • Structure of the Polβ-XRCC1-LigIIIα gap-repair complex

Double Strand Break Repair

We are studying the structure and function of proteins and complexes involved in repair of double-strand breaks by the Homologous Recombination and Non-Homologous End Joining pathways.
Ongoing projects include :
  • Structure of the NHEJ DSB ligation complex
  • Structure and function of PALB2

DNA Damage Signalling

We are studying the assembly, regulation and interactions of proteins and complexes that signal the presence of DNA damage to the systems that regulate progress through the cell cycle.
Ongoing projects include :
  • DNA-loading of the Rad9-Rad1-Hus1 checkpoint clamp by the RAD-RFC complex
  • Structural basis for complex formation by the TopBP1 checkpoint mediator
  • Architecture of the 9-1-1 - ATR DNA damage checkpoint supercomplex