School of Life Sciences

Osborne Lab

Single Molecule Spectroscopy

RSBiogPicResearch in our lab focuses on the development and application of ultra-sensitive optical techniques for the detection and manipulation of single molecules. Representing the ultimate level of sensitivity in the analysis and control of matter, single molecule techniques have many advantages over conventional ensemble methods. The ability to interrogate a molecular population, molecule by molecule, can provide information on both the static disorder, or heterogeniety within the ensemble, at any instant in time and dynamic disorder in the population as well as individual molecules as the molecular state evolves in time. Furthermore single molecule measurements do not require the synchronisation of molecular populations, the subsequent dephasing and the statistical averaging associated that can mask rare and fast molecular processes using ensemble techniques.

We have implemented a number of novel techniques including defocused dipole imaging, near-TIRF and Fourier transform FCS, dispersed-fluorescence spectroscopy on customised laser microscopy platforms to investigate a diverse range of molecular systems including; protein-ligand interactions, DNA and protein folding dynamics, protein-protein interactions in yeast cells and quantum dot photoactivation, blinking and bluing.



Dr Mark Osborne

Lecturer in Physical Chemistry

University of Sussex
Chichester III building
Brighton, BN1 9QJ

T +44 1273 678328

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Science guy at Sussex Uni. Thinking single molecules and more. Ts n RTs for interest, not endorsement

Of course in the multiverse, the simulation is another yeast's reality, right? #riddlemethis…

@simurdic @illumina Bojan, shame we were north at the Bahia, Mission Bay. San Diego was gorgeous. Look where Solex……

Now wait for the first stag do victim found strapped to the prop. #PropPoweredBrighton…

Blinking, brightening, blueing, bleaching models may help make master CFQDs for TV tech.….……

Efficient excitons essential to exciting new TV nanotech. #QDot #spectroscopy…

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