School of Life Sciences

Osborne Lab


Single Molecule Spectroscopy

RSBiogPicResearch in our lab focuses on the development and application of ultra-sensitive optical techniques for the detection and manipulation of single molecules. Representing the ultimate level of sensitivity in the analysis and control of matter, single molecule techniques have many advantages over conventional ensemble methods. The ability to interrogate a molecular population, molecule by molecule, can provide information on both the static disorder, or heterogeniety within the ensemble, at any instant in time and dynamic disorder in the population as well as individual molecules as the molecular state evolves in time. Furthermore single molecule measurements do not require the synchronisation of molecular populations, the subsequent dephasing and the statistical averaging associated that can mask rare and fast molecular processes using ensemble techniques.

We have implemented a number of novel techniques including defocused dipole imaging, near-TIRF and Fourier transform FCS, dispersed-fluorescence spectroscopy on customised laser microscopy platforms to investigate a diverse range of molecular systems including; protein-ligand interactions, DNA and protein folding dynamics, protein-protein interactions in yeast cells and quantum dot photoactivation, blinking and bluing.

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Contact

Dr Mark Osborne

Lecturer in Physical Chemistry

University of Sussex
Chichester III building
Falmer
Brighton, BN1 9QJ

E m.osborne@sussex.ac.uk
T +44 1273 678328

go to Dr Mark Osborne's profile

Twitter

Science guy at Sussex Uni. Thinking single molecules and more. Ts n RTs for interest, not endorsement

And here it is... if the CO2 don't getcha, we'll soon be cooking on gas and all that fertiliser won't just stink!… twitter.com/i/web/status/9…

10 yrs since i taught this doom in Atmospheric Chemistry F1022. Lecture 6 slide 2. IPCC predictions to 2100. So lo… twitter.com/i/web/status/9…

Not what the academic signed up to, surely! The last bastions bust their integrity. theguardian.com/commentisfree/…

Guest appearance from a rainbow moon, thru the prism of an altocumulus front at #prestonpark #fireworks2017twitter.com/i/web/status/9…

Hal tests his scare ahead of Hallows' eve. With or without ears? The Nell v dad debate. More sinister with, I say!… twitter.com/i/web/status/9…

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