Publications from the lab to date

Professor Simon Morley

James V., Zhang Y., Foxler D.E., de Moor C.H., Kong Y.W., Webb T.M., Self T.J., Feng Y., Lagos D., Chu C.Y., Rana T.M., Morley S.J., Longmore G.D., Bushell M., and Sharp T.V. (2010) LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proc Natl Acad Sci USA. 107, 12499-504.

Bhatia P., Menigatti M., Brocard M., Morley S.J., Ferrari S. (2010) Mitotic DNA damage targets the Aurora A/TPX2 complex. Cell Cycle. 9, 4592-9.

Willett M., Pollard H.J., Vlasak M., and Morley S.J. (2010) Localisation of translation initiation factors to talin/beta3-integrin-enriched adhesion complexes in spreading and migrating mammalian cells. Biol. Cell 102, 265-276.

Willett M., Cowan J.L., Vlasak M., Coldwell M.J., and Morley S.J. (2009) Inhibition of mammalian target of rapamycin (mTOR) signalling in C2C12 myoblasts prevents myogenic differentiation without affecting the hyperphosphorylation of 4E-BP1. Cell Signal. 21,1504-12.

Morley SJ, Willett M (2009). Kinky binding and SECsy insertion. Mol. Cell 35, 396-8.

Spriggs K.A, Cobbold L.C, Jopling C.L, Cooper R, Wilson L.A, Stoneley M, Coldwell M.J, Poncet D, Shen Y.C, Morley S.J, Bushell M, Willis A.E. (2009) Canonical initiation factor requirements for the myc family of internal ribosome entry segments. Mol. Cell Biol. 29,1565-74.

Morley, S.J. and Willett M. (2008). Signaling pathways open the GAITway to translational control. Dev Cell. 15, 639-40.

Morley, S.J., and Coldwell, M.J. (2008). A cunning stunt: An alternative mechanism of eukaryotic translation initiation for cIAP2. Science Sig. 1, pe32-34.

Wheatley, S.P., Barrett, R.M., Andrews, P.D., Medema, R.H., Morley, S.J., Swedlow, J.R., Lens, S.M. (2007) Phosphorylation by aurora-B negatively regulates survivin function during mitosis. Cell Cycle, 6, 1220-30.

Morley, S.J., and Coldwell, M.J. (2007). Matters of life and death: translation initiation during apoptosis, in Translational Control in Biology and Medicine (Matthews, M.B., Hershey, J.W.B., Sonenberg, N, eds), pp433-458. Cold Spring Harbor Lab Press, New York, USA

Hinton, T.M., Coldwell, M.J., Carpenter, G.A., Morley, S.J., and Pain, V.M. (2007). Functional Analysis of Individual Binding Activities of the Scaffold Protein eIF4G.
J. Biol. Chem., 282, 1695-708.

Coldwell, M.J., and Morley, S.J. (2006). Specific isoforms of translation initiation factor 4GI show differences in translational activity. Mol. Cell Biol. 26, 8448-60.

Willett, M., Flint, S.A., Morley, S.J., and Pain, V.M. (2006).  Compartmentalisation and localisation of the translation initiation factor (eIF)4F complex in normally growing fibroblasts. Exp. Cell Res. 312, 2942-53.

DeGracia, D.J., Rafols, J.A., Morley, S.J., and Kayali, F. (2006) Immunohistochemical mapping of total and phosphorylated eukaryotic initiation factor 4G in rat hippocampus following global brain ischemia and reperfusion. Neuroscience 139, 1235-1248.

Ling, J., Morley, S.J., and Traugh, J.A. (2005) Inhibition of cap-dependent translation via phosphorylation of eIF4G by protein kinase Pak2. EMBO J. 24, 4094-105.

Morley, S.J., Coldwell, M.J., and Clemens, M.J. (2005) Initiation factor modifications in the pre-apoptotic phase. Cell Death Differ. 12, 571-584.

Naegele, S., and Morley, S.J. (2004) Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic shock. J. Biol. Chem. 279, 46023-46034.

Cowan, J.L., and Morley, S.J. (2004) The proteasome inhibitor, MG132, promotes the reprogramming of translation in C2C12 myoblasts and facilitates the association of hsp25 with the eIF4F complex. Eur. J. Biochem. 271, 3596-3611.

Mosaheb, S., Hashemzadeh-Bonehi, L., Cairns, N.J., Kay, J.E., Morley, S.J., Rulten, S.L., and Thorpe, J.R. (2004) Shortfalls in the peptidyl-prolyly cis-trans isomerase protein pin1 in neurons are associated with fronto-temporal dementias. Neurobiol. Disease 17, 237-249.

Medina-Palazon, C., Bernard, E., Frost, V., Morley, S.J., and Sinclair, A.J. (2004) KIPase is a novel caspase-like activity associated with cell proliferation. Eur. J. Biochem. 271, 2716-2723.

Goggin, M.M., Nelsen, C.J., Kimball, S.R., Jefferson, L.S., Morley, S.J., and Albrecht, J.H. (2004) Rapamycin-sensitive induction of eukaryotic initiation factor 4F in regenerating mouse liver. Hepatol. 40, 537-544.

Coldwell, M.J., Hashemzadeh-Bonehi, L., Hinton, T.M., Morley, S.J., and Pain, V.M. (2004) Expression of fragments of translation initiation factor eIF4GI reveals a nuclear localisation signal within the N-terminal apoptotic cleavage fragment N-FAG. J. Cell Sci. 117, 2545-2555.

Walsh, D., Meleady, P., Power, B., Morley, S.J. and Clynes, M. (2003) Increased levels of the translation initiation factor, eIF4E, in differentiating epithelial lung tumour cell lines. Differentiation 71, 126-134.

Morley, S.J., and Naegele, S. (2003) Phosphorylation of initiation factor (eIF) 4E is resistant to SB203580 in cells expressing a drug-resistant mutant of stress-activated protein kinase 2a/p38. Cell. Signalling 15, 741-749.

Hashemzadeh-Bonehi, L., Curtis, P.S., Morley, S.J., Thorpe, J.R., and Pain, V.M. (2003) Overproduction of a conserved domain of fission yeast and mammalian translation initiation factor eIF4G causes aberrant cell morphology and results in disruption of the localization of F-actin and the organization of microtubules. Genes to Cells 8, 163-178.

Ling, J., Morley, S.J., Pain, V.M., Marzluff, W.F., and Gallie, D.R. (2002) The histone 3 terminal stem loop binding protein enhances translation through a functional and physical interaction with eIF4G and eIF3. Mol. Cell. Biol. 22, 7853-7867.

Morley, S.J., and Naegele, S. (2002) Phosphorylation of eukaryotic initiation factor (eIF) 4E is not required for de novo protein synthesis following recovery from hypertonic stress in human kidney cells. J. Biol. Chem. 277, 32855-32859.

Ohlmann, T., Prevot, D., Decimo, D., Roux, F., Garin, J., Morley, S.J., and Darlix, J.L. (2002) In vitro cleavage of eIF4GI but not eIF4GII by HIV-1 protease and its effects on translation in rabbit reticulocyte lysate system. J. Mol. Biol. 318, 9-20.

Jeffrey, I.W., Bushell, M., Tilleray, V.J., Morley, S.J., and Clemens, M.J. (2002) Inhibition of protein synthesis in apoptosis: differential requirements by the Tumor Necrosis Factor family and a DNA-damaging agent for caspases and double-stranded RNA-dependent protein kinase. Cancer Res. 62, 2272-2280.

Woods, A.J., Roberts, M.S, Choudhary, J., Barry, S.T., Mazaki, Y., Sabe, H., Morley, S.J., Critchley, D.R. and Norman, J.C. (2002) Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells. J. Biol. Chem. 277, 6428-6437.

Morley, S.J. (2001) The regulation of eIF4F during cell growth and cell death, in Signaling Pathways for Translation (Rhoads, R.E. (ed.)), pp1-38, Springer-Verlag, Berlin.

McKendrick, L., Morley, S.J., Pain, V.M., Jagus, R., and Joshi, B. (2001) Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo. Eur. J. Biochem. 268, 5375-85.

Morley, S.J., and Pain, V.M. (2001) Proteasome inhibitors and immunosuppressive drugs promote the cleavage of eIF4GI and eIF4GII by caspase-8-independent mechanisms in Jurkat T cell lines. FEBS Letts. 503, 206-212.

Ali, I.K., McKendrick, L., Morley, S.J., and Jackson, R.J. (2001) Truncated initiation factor eIF4G lacking an eIF4E binding site can support capped mRNA translation. EMBO J. 20, 4233-4242.

Ali, I.K., McKendrick, L., Morley, S.J., and Jackson, R.J. (2001) Activity of the Hepatitis A virus IRES requires association between the cap-binding translation initiation factor (eIF4E) and eIF4G. J. Virol. 75, 7854-7863.

Bushell, M., Wood, W., Carpenter, G., Pain, V.M., Morley, S.J., and Clemens, M.J. (2001) Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral-mediated proteases. J. Biol. Chem. 276, 23922-23928.

McKendrick, L., Thompson, E., Ferreira, J., Morley, S.J., and Lewis, J.D. (2001) Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m7 guanosine cap. Mol. Cell Biol. 21, 3632-3641.

Thorpe, J.R., Morley, S.J., and Rulten, S.L. (2001) Utilising the peptidyl-prolyl cis-trans isomerase Pin1 as a probe of its phosphorylated target proteins: examples of binding to nuclear proteins in a human kidney cell line and to Tau in Alzheimers diseased brain. J. Histochem. Cytochem. 49, 97-107.

Gallie, D.R., Ling, J., Niepel, M., Morley, S.J., and Pain, V.M. (2000) The role of 5-leader length, secondary structure and PABP concentration on cap and poly(A) tail function during translation in Xenopus oocytes. Nucl. Acid Res. 28, 2943-2953

Morley, S.J., Jeffrey, I.W., Bushell, M., Pain, V.M., and Clemens, M.J. (2000) Differential requirements for caspase-8 activity in the mechanism of phosphorylation of eIF2, cleavage of eIF4GI and signalling events associated with the inhibition of protein synthesis in apoptotic Jurkat T cells. FEBS Lett. 477, 229-236.

Bushell, M., Poncet, D., Marissen, W.E., Flotow, H., Lloyd, R.E., Clemens, M.J., and Morley, S.J., (2000) Cleavage of polypeptide chain initiation factor eIF4GI during apoptosis in lymphoma cells: characterisation of an internal fragment generated by caspase-3 mediated cleavage. Cell Death Differ. 7, 628-636.

Clemens, M.J., Bushell, M., Jeffrey, I.W., Pain, V.M. and Morley, S.J. (2000) Translation initiation factor moficiations and the regulation of protein synthesis in apoptotic cells. Cell Death Differ. 7, 603-615.

Bushell, M., Wood, W., Clemens, M.J., and Morley, S.J. (2000). Changes in integrity and association of eukaryotic protein synthesis initiation factors during apoptosis. Eur. J. Biochem. 267, 1083-1091.

Fraser, C.S., Pain, V.M., and Morley, S.J. (1999). Cellular stress in Xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with Poly(A)-binding protein. Biochem. J. 342, 519-526.

McKendrick, L., Pain, V.M., and Morley, S.J. (1999). Translation initiation factor 4E. Int. J. Biochem. Cell Biol. 31, 31-5.

Bushell, M., McKendrick, L., Janicke, R.U., Clemens, M.J., and Morley, S.J. (1999). Caspase-3 is necessary and sufficient for cleavage of protein synthesis initiation factor eIF4G during apoptosis. FEBS Lett. 451, 332-336.

Fraser, C.S., Pain, V.M., and Morley, S.J. (1999). The association of initiation factor (eIF) 4F with poly(A)-binding protein is enhanced in serum-stimulated Xenopus kidney cells. J. Biol. Chem. 274, 196-204.

Morley, S.J., McKendrick, L., and Bushell, M. (1998). Cleavage of translation initiation factor 4G (eIF4G) during anti-Fas IgM-induced apoptosis does not require signalling through p38 Mitogen-activated protein (MAP) kinase. FEBS Lett. 438, 41-48.

Clemens, M.J., Bushell, M., and Morley, S.J. (1998). Degradation of eukaryotic polypeptide chain initiation factor (eIF) 4G in response to induction of apoptosis in human lymphoma cell lines. Oncogene 17, 2921-2931.

Pain, V.M., Patrick, T.D., Cox, R., and Morley, S.J. (1998) Analysis of translational activity of extracts from oocytes and eggs of Xenopus Laevis. Meth. in Mol. Biol. 77, p195-210.

Rau, M., Ohlmann, T., Pain, V.M., and Morley, S.J. (1998). A fractionated reticulocyte lysate system for studies on protein synthesis initiation factors. Meth. in Mol. Biol. 77, p211-226.

McKendrick, L., Pain, V.M., and Morley, S.J. (1998). Molecules in focus: translation initiation factor eIF4E. Int. J. Biochem. Cell Biol. 31, 31-35.

Hashemolhosseini, S., Nagamine, Y., Morley, S.J., Desrivieres, S., Mercep, L., and Ferrari, S. (1998). Rapamycin inhibition of the G1 to S transition is mediated by effects on cyclin D1 mRNA and protein stability. J. Biol. Chem. 273, 14424-14429.

Morley, S.J. (1997). Signalling through either the p38 or ERK mitogen-activated protein (MAP) kinase pathway is obligatory for phorbol ester and T cell receptor complex (TCR-CD3)-stimulated phosphorylation of initiation factor (eIF) 4E in Jurkat T cells. FEBS Lett. 418, 327-332.

Morley, S.J., Curtis, P.S. and Pain, V.M. (1997). eIF4G: Translations mystery factor begins to yield its secrets. RNA 3, 1085-1104.

Morley, S.J. and McKendrick, L. (1997). Involvement of stress-activated protein kinase and p38/RK mitogen activated protein kinase signaling pathways in the enhanced phosphorylation of initiation factor 4E in NIH 3T3 cells. J. Biol. Chem. 272, 17887-17893.

Morley, S.J. (1997). REVIEW; Intracellular signalling pathways regulating initiation factor phosphorylation during the activation of cell growth. Biochem. Soc. Trans. 25, 503-509.

Ohlmann, T., Pain, V.M., Wood, W., Rau, M., and Morley, S.J. (1997). The proteolytic cleavage of eukaryotic initiation factor (eIF) 4G is prevented by eIF4E binding protein (PHAS-I; 4E-BP1) in the reticulocyte lysate. EMBO J. 16, 844-855.

Morley, S.J. (1996). Regulation of components of the translational machinery by protein phosphorylation; in Protein phosphorylation in cell growth regulation (Clemens, M.J., ed.) Harwood Academic Publishers, Amsterdam. p197-225.

Rau, M., Ohlmann, T., Morley, S.J. and Pain, V.M. (1996). A re-evaluation of the cap binding protein eIF4E, as a rate-limiting factor for initiation of translation in reticulocyte lysate. J. Biol. Chem. 271, 8983-8990.

Ohlmann, T., Rau, M., Pain, V.M. and Morley, S.J. (1996). The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E. EMBO J. 15, 1371-1382.

Frost, V., Morley, S.J., Mercep, L., Meyer, T., and Ferrari, S. (1995). The phosphodiesterase inhibitor SQ20006 selectively blocks mitogen activation of p70S6K and transition to S phase of the cell division cycle without affecting the steady state phosphorylation of eIF4E. J. Biol. Chem. 270, 26698-26706.

Morley, S.J., and Pain, V.M. (1995). Translational regulation during the activation of porcine peripheral blood lymphocytes; association and phosphorylation of the and subunits of the initiation factor complex eIF4F. Biochem. J. 312, 627-635.

Morley, S.J., and Pain, V.M. (1995). Hormone-induced meiotic maturation in Xenopus oocytes occurs independently of p70 S6K activation and is associated with enhanced initiation factor (eIF)-4F phosphorylation and complex formation. J. Cell Sci. 108, 1751-1760.

Ohlmann, T., Rau, M., Morley, S.J., and Pain, V.M. (1995). Proteolytic cleavage of eIF-4 in the reticulocyte lysate inhibits translation of capped mRNAs but enhances that of uncapped mRNAs. Nucleic Acids Res. 23, 334-340.

Joshi, B., Morley, S.J., Rhoads, R.E., and Pain, V.M. (1995). Inhibition of protein synthesis by the haem-controlled eIF-2 kinase leads to the appearance of mRNA-containing 48S complexes that contain eIF-4E but lack methionyl-tRNAf . Eur. J. Biochem. 228, 31-38.

Morley, S.J. (1994). REVIEW; Signal transduction mechanisms in the regulation of protein synthesis. Mol. Biol. Reports 19, 221-231.

Morley, S.J., and Thomas, G. (1993). Intracellular messengers and the control of protein synthesis. In: Taylor C.W. (ed) The international encyclopedia of pharmacology and therapeutics: Intracellular messengers. Permagon, New York, p447-483.

Morley, S.J., Rau, M., Kay, J.E. and Pain, V.M. (1993). Increased phosphorylation of eukaryotic initiation factor eIF-4 during early activation of T lymphocytes correlates with Increased initiation factor 4F complex formation. Eur. J. Biochem., 218, 39-48.

Morley, S.J. and Traugh, J.A. (1993). Activation of protein synthesis in 3T3-L1 cells in response to insulin correlates with increased phosphorylation of initiation factor eIF-4F and ribosomal protein S6. Biochimie 75, 985-989.

Ferrari, S., Bannworth, W., Morley, S.J., Totty, N.F., and Thomas, G. (1992). Activation of p70s6k is associated with phosphorylation of four clustered sites displaying Ser/Thr Pro motifs. Proc. Natl. Acad. Sci. USA 89, 7282-86.

Lane, H.A., Morley, S.J., Doree, M., Kozma, S.C. and Thomas G.(1992). Early activation of a Xenopus laevis p70s6k homologue following progesterone-induced meiotic maturation. EMBO J. 11, 1743-49.

Morley, S.J., and Thomas, G. (1991). REVIEW; Intracellular messengers and the control of protein synthesis. Pharmac. Ther. 50, 291-319.

Morley, S.J., Etchison, D.E., Dever, T.E., and Traugh, J.A. (1991). Phosphorylation of eIF-4F by protein kinase C or multipotential S6 kinase stimulates protein synthesis at initiation. J. Biol. Chem., 266, 4669-4672.

Tuazon, P.T., Morley, S.J., Rhoads, R.E., Dever, T.E., and Traugh, J.A.(1990). Association of initiation factor eIF-4E in the cap binding complex (eIF-4F) is critical for phosphorylation by protein kinase C. J. Biol. Chem. 265, 10617-10621.

Morley, S.J., and Traugh, J.A. (1990). Phosphorylation of eIF-4F and eIF-4B is stimulated via a protein kinase C-dependent and independent pathway in 3T3-L1 cells. J. Biol.Chem., 265, 10611-10616.

Morley, S.J. and Hershey, J.W.B. (1990). A fractionated reticulocyte lysate retains high efficiency for protein synthesis. Biochimie, 72, 259-64.

Morley, S.J., and Traugh, J.A. (1989). Phorbol esters stimulate phosphorylation of eukaryotic intiation factors 3, 4B and 4F. J. Biol. Chem. 264, 2401-2404.

Morley, S.J., Buhl, W.-J., and Jackson, R.J. (1985). A rabbit reticulocyte factor which stimulates protein synthesis in several mammalian cell-free systems. Biochim. Biophys. Acta 825, 57-69.

Morley, S.J., and Jackson, R.J. (1985). Preparation and properties of an improved cell-free protein synthesis system from mammalian liver. Biochim. Biophys. Acta 825, 45-56.








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Publications with abstracts and links