Dr Helfrid Hochegger


Booth AJ, Yue Z, Eykelenboom JK, Stiff T, Luxton GG, Hochegger H & Tanaka TU (2019) Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis. bioRxiv:459750 

Hégarat N, Crncec A, Suarez Peredo Rodriguez MF, Echegaray Iturra F, Gu Y, Lang PF, Barr AR, Bakal C, Kanemaki MT, Lamond AI, Novák B, Ly T & Hochegger H (2018) Cyclin A triggers Mitosis either via Greatwall or Cyclin B. bioRxiv:501684 

Eykelenboom, J. K., Gierlinski, M, Zuojun Yue, Hegarat, N. Pollard, H. Fukagawa, T., Hochegger H., Tanaka, T. Live-cell imaging of marked chromosome regions reveals dynamics of mitotic chromosome resolution and compaction. (2019) Journal of Cell biology  doi: 10.1083/jcb.201807125

Rata S, Rodriguez MFSP, Joseph S, Peter N, Iturra FE, Yang F, Madzvamuse A, Ruppert JG, Samejima K, Platani M, Alvarez-Fernández M, Malumbres M, Earnshaw WC, Novák B & Hochegger H (2018) Two Interlinked Bistable Switches Govern Mitotic Control in Mammalian Cells. Curr. Biol.28(23): 3824–3832.e6 (Highlighted by Goldberg ML, Curr. Biol.28(23):1342-1345.e6)

Lemmens B, Hégarat N, Akopyan K, Sala-Gaston J, Bartek J, Hochegger H* & Lindqvist A (2018) DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Molecular Cell 71:117–128.e3 (*joint corresponding author)

Eykelenboom, J. K., Gierlinski, M, Zuojun Yue, Hegarat, N. Pollard, H. Fukagawa, T., Hochegger H., Tanaka, T. Live-cell imaging of marked chromosome regions reveals dynamics of mitotic chromosome resolution and compaction. bioRxiv 305391 (2018). doi:10.1101/305391

Hégarat, N., Rata, S., & Hochegger, H. (2016). Bistability of mitotic entry and exit switches during open mitosis in mammalian cells. BioEssays, 38(7), 627–643.

Ocasio, C. A. et al. H. Hochegger*, Antony Oliver* A first generation inhibitor of human Greatwall kinase, enabled by structural and functional characterisation of a minimal kinase domain construct. Oncotarget 5, 71182–71197 (2016). Joint corresponding authors

Bailey, L. J., Bianchi, J., Hégarat, N., Hochegger, H. & Doherty, A. J. PrimPol-deficient cells exhibit a pronounced G2 checkpoint response following UV damage. Cell Cycle 15, 908–918Mannino M, Gomez-Roman N, Hochegger H*, Chalmers AJ (2014) Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: Implications for stem cell mitosis and centrosome dynamics. Stem Cell Res 13: 135-143. * corresponding author

Kustatscher G, Hegarat N, Wills KL, Furlan C, Bukowski-Wills JC, Hochegger, H.*, Rappsilber J, (2014) Proteomics of a fuzzy organelle: interphase chromatin. EMBO J 33: 648-664. * joint corresponding author

Hegarat N, Vesely C, Vinod PK, Ocasio C, Peter N, Gannon J, Novak B, Hochegger H, (2014) PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic exit. PLoS Genet 10: e1004004.

Hochegger H, Hegarat N, Pereira-Leal JB (2013) Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle. Open Biol 3: 120185.

Tsubouchi T, Soza-Ried J, Brown K, Piccolo FM, Cantone I, Landeita A, Bagci H, Hochegger H, Merkenschlager M, Fisher AG, (2013) DNA synthesis is required for reprogramming mediated by stem cell fusion. Cell 152: 873-883.

Hegarat, N., Smith, E., Nayak, G., Takeda, S., Eyers, P.A. and Hochegger, H. (2011) Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics. J. Cell Biol., 195, 1103-1113.

Silva, M.C., Bodor, D.L., Stellfox, M.E., Martins, N.M., Hochegger, H., Foltz, D.R. and Jansen, L.E. (2011) Cdk Activity Couples Epigenetic Centromere Inheritance to Cell Cycle Progression. Dev. Cell.

Smith, E., Hegarat, N., Vesely, C., Roseboom, I., Larch, C., Streicher, H., Straatman, K., Flynn, H., Skehel, M., Hirota, T., Kuriyama, R. and Hochegger, H. (2011) Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1. Embo J., 30, 2233-2245.

Steere, N., Wagner, M., Beishir, S., Smith, E., Breslin, L., Morrison, C.G., Hochegger, H. and Kuriyama, R. (2011) Centrosome amplification in CHO and DT40 cells by inactivation of cyclin-dependent kinases. Cytoskeleton (Hoboken), 68, 446-458.

Xu, N., Hegarat, N., Black, E.J., Scott, M.T., Hochegger, H. and Gillespie, D.A. (2010) Akt/PKB suppresses DNA damage processing and checkpoint activation in late G2. J Cell Biol, 190, 297-305.

Bourke, E., Brown, J.A., Takeda, S., Hochegger, H. and Morrison, C.G. (2010) DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2. Oncogene, 29, 616-624.

Cinnamon, Y., Feine, O., Hochegger, H., Bershadsky, A. and Brandeis, M. (2009) Cellular contractility requires ubiquitin mediated proteolysis. PLoS One, 4, e6155.

Hochegger, H., Takeda, S. and Hunt, T. (2008) Cyclin-dependent kinases and cell-cycle transitions: does one fit all? Nat. Rev. Mol. Cell Biol., 9, 910-916.

Saberi, A., Hochegger, H., Szuts, D., Lan, L., Yasui, A., Sale, J.E., Taniguchi, Y., Murakawa, Y., Zeng, W., Yokomori, K., Helleday, T., Teraoka, H., Arakawa, H., Buerstedde, J.M. and Takeda, S. (2007) RAD18 and poly(ADP-ribose) polymerase independently suppress the access of nonhomologous end joining to double-strand breaks and facilitate homologous recombination-mediated repair. Mol Cell Biol, 27, 2562-2571.

Murakawa, Y., Sonoda, E., Barber, L.J., Zeng, W., Yokomori, K., Kimura, H., Niimi, A., Lehmann, A., Zhao, G.Y., Hochegger, H., Boulton, S.J. and Takeda, S. (2007) Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. Cancer Res, 67, 8536-8543.

Hochegger, H., Dejsuphong, D., Sonoda, E., Saberi, A., Rajendra, E., Kirk, J., Hunt, T. and Takeda, S. (2007) An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J. Cell Biol., 178, 257-268.

Fisher, A.E., Hochegger, H., Takeda, S. and Caldecott, K.W. (2007) Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase. Mol. Cell Biol., 27, 5597-5605.

Yoshimura, M., Kohzaki, M., Nakamura, J., Asagoshi, K., Sonoda, E., Hou, E., Prasad, R., Wilson, S.H., Tano, K., Yasui, A., Lan, L., Seki, M., Wood, R.D., Arakawa, H., Buerstedde, J.M., Hochegger, H., Okada, T., Hiraoka, M. and Takeda, S. (2006) Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell, 24, 115-125.

Wu, X., Takenaka, K., Sonoda, E., Hochegger, H., Kawanishi, S., Kawamoto, T., Takeda, S. and Yamazoe, M. (2006) Critical roles for polymerase zeta in cellular tolerance to nitric oxide-induced DNA damage. Cancer Res, 66, 748-754.

Sonoda, E., Hochegger, H., Saberi, A., Taniguchi, Y. and Takeda, S. (2006) Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. DNA Repair (Amst), 5, 1021-1029.

Hochegger, H. and Takeda, S. (2006) Phenotypic analysis of cellular responses to DNA damage. Subcell Biochem., 40, 313-325.

Hochegger, H., Dejsuphong, D., Fukushima, T., Morrison, C., Sonoda, E., Schreiber, V., Zhao, G.Y., Saberi, A., Masutani, M., Adachi, N., Koyama, H., de Murcia, G. and Takeda, S. (2006) Parp-1 protects homologous recombination from interference by Ku and Ligase IV in vertebrate cells. Embo J., 25, 1305-1314.

Yonetani, Y., Hochegger, H., Sonoda, E., Shinya, S., Yoshikawa, H., Takeda, S. and Yamazoe, M. (2005) Differential and collaborative actions of Rad51 paralog proteins in cellular response to DNA damage. Nucleic Acids Res, 33, 4544-4552.

Nojima, K., Hochegger, H., Saberi, A., Fukushima, T., Kikuchi, K., Yoshimura, M., Orelli, B.J., Bishop, D.K., Hirano, S., Ohzeki, M., Ishiai, M., Yamamoto, K., Takata, M., Arakawa, H., Buerstedde, J.M., Yamazoe, M., Kawamoto, T., Araki, K., Takahashi, J.A., Hashimoto, N., Takeda, S. and Sonoda, E. (2005) Multiple repair pathways mediate tolerance to chemotherapeutic cross-linking agents in vertebrate cells. Cancer Res, 65, 11704-11711.

Kikuchi, K., Taniguchi, Y., Hatanaka, A., Sonoda, E., Hochegger, H., Adachi, N., Matsuzaki, Y., Koyama, H., van Gent, D.C., Jasin, M. and Takeda, S. (2005) Fen-1 facilitates homologous recombination by removing divergent sequences at DNA break ends. Mol. Cell Biol., 25, 6948-6955.

Yamazoe, M., Sonoda, E., Hochegger, H. and Takeda, S. (2004) Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40. DNA Repair (Amst), 3, 1175-1185.

Mizutani, A., Okada, T., Shibutani, S., Sonoda, E., Hochegger, H., Nishigori, C., Miyachi, Y., Takeda, S. and Yamazoe, M. (2004) Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells. Cancer Res, 64, 3144-3147.

Hochegger, H., Sonoda, E. and Takeda, S. (2004) Post-replication repair in DT40 cells: translesion polymerases versus recombinases. Bioessays, 26, 151-158.

Sohail, M., Hochegger, H., Klotzbucher, A., Guellec, R.L., Hunt, T. and Southern, E.M. (2001) Antisense oligonucleotides selected by hybridisation to scanning arrays are effective reagents in vivo. Nucleic Acids Res, 29, 2041-2051.

Hochegger, H., Klotzbucher, A., Kirk, J., Howell, M., le Guellec, K., Fletcher, K., Duncan, T., Sohail, M. and Hunt, T. (2001) New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation. Development, 128, 3795-3807.