School of Life Sciences

NMR

The School is the home to three Varian VNMRS solution-state NMR spectrometers, providing comprehensive NMR facilities to the researchers within the School. All three spectrometers are equipped with pulsed field gradients and are capable of variable temperature operation.

Two instruments are available to researchers on an open-access basis: the 400 MHz spectrometer mainly supports inorganic chemistry and is equipped with an X{1H-19F} broadband probe, whereas the 500 MHz instrument handles the majority of the small molecule structure elucidation tasks using an inverse 1H{13C} probe.

Our main research instrument is the 4-channel 600 MHz spectrometer, equipped with both an X{1H-19F} broadband probe and an 1H{13C/15N/31P/2H} AutoPenta Probe for biological samples. This instrument is well suited for both small molecule work and structural studies of small to medium-sized biopolymers.

For further information please contact Dr Iain Day (I.J.Day@sussex.ac.uk

What is the NMR Facility used for?

Nuclear Magnetic Resonance (NMR) spectroscopy is a vital technique for determining the structure of both small molecules and small to medium-sized proteins and nucleic acids. The School has three NMR spectrometers primarily focused on small molecule work, but with the capabilities for protein NMR spectroscopy.

The main focus of the NMR facility is the structure determination of the compounds synthesised by the research groups across chemistry and drug discovery. We have a wide variety of experiments available which allow us to determine connectivities, i.e. how the atoms are put together in a given molecule. We can also probe the dynamics of a system, are regions of a molecule more or less flexible, what is the aggregation state of a system etc.

On the biological side, we can acquire data on labelled samples which allow users to perform preliminary experiments showing protein stability, sample quality etc, and allow them to access to more specialist facilities, such as requesting time on national > 900MHz spectrometers.

Who can use it and how do you gain access?

The NMR facility is available to researchers across Life Sciences, and more broadly within the University. Access to the open-access instruments is available following training from the Facility Manager, Dr Iain Day, (second-hand training from other group members is strictly prohibited). These instruments are either first-come-first served via a robot autosampler, or booked an on-line booking system.

Access to more specialist experiments (detailed structure elucidation, biomolecular NMR, diffusion experiments etc) is via discussion with the NMR facility manager in the first instance.

Find us

The NMR facility is located on the ground floor of Chichester 2, in room 2R116. Note, due to the presence of strong magnetic fields within the laboratory, all visitors must report to the facility manager prior to accessing the facility. Persons with pacemakers, and some metallic implants, will not be allowed into the facility.