School of Life Sciences

Electron Microscopy Imaging Centre

The Electron Microscopy Imaging Centre (EMC) of the School of Life Sciences was renewed in 2016 by the Wellcome Trust and RM Phillips trust donation. We are aiming to support and develop structural biology research projects across the University of Sussex. These range from atomic and molecular particle analysis to the characterization of tissue and cell ultrastructure by using ransmission electron microscopy (TEM), cryoEM, tomography and integrative imaging approaches.

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We are a cryoEM imaging centre with access to eBIC using a BAG allocation for high resolution data acquisition.

About the microscope

Our JEOL JEM1400-Plus (120kV, LaB6) is equipped with a Gatan OneView 4K camera optimized for both sensitivity and speedThis latest generation of high frame rate CMOS sensor allows automatic real time drift correction for high resolution 2D and 3D image analysis. The microscope is also fitted with high contrast imaging objective apertures for biological samples and set up for low dose image acquisition and tomography of thin samples (Serial EM and Recorder suites)

Images from our microscope

Make a booking

You can book various instruments, including the microscope once training is completed.

Contact

Pascale Schellenberger is head of the EMC. You can email Dr Schellenberger.

Browse instruments

JEOL JEM-1400Plus

  • 120 kV/TEM with Lab6 and Gatan OneView 4K CMOS digital camera
  • GMS3 (Gatan)
  • ShotMeister for high resolution 2D montages (JEOL)
  • Recorder for Tomography (JEOL)
  • Serial EM for low dose CryoEM data acquisition

Sample holders

  • Cryo-holders, Gatan 626 single tilt
  • Single specimen holder
  • Quartet holder
  • High tilt holder

Sample preparation

  • High pressure freezer : HPF-ICE (Leica)
  • Automatic freeze substitution : AFS2 with FSP unit (Leica)
  • Cryo-plunger : CP3 ( Gatan) and GP2 (Leica)
  • Negative staining preparation area
  • Leica Ultracut Ultramicrotome

Applications and data acquisition software

  • Gatan GMS3
  • JEOL ShotMeister for high resolution 2D montages
  • JEOL Recorder for Tomography
  • Serial EM for low dose CryoEM data acquisition.
Find us

Directions

The facility is based in the John Maynard Smith building (JMS), on main ground floor research corridor. Read our directions by train, buses or car on how to get to the University campus and walk to the John Maynard Smith Building.

Car park 1 on Science Road and car park 3, with pay-and-display machines, are the closest to the JMS building.

Address

Electron Microscopy Imaging Centre
School of Life Sciences
John Maynard Smith Building
University of Sussex
Brighton, BN1 9QG
United Kingdom.