Zeiss AxioVision

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General information

  Zeiss upright microscope with motorized filter wheels, manual XY stage, manual focusing and EMCCD camera.

  • 2D brightfield and multi-channel fluorescence imaging of fixed/live samples
Microscope details
  • Microscope frame: Zeiss Axioplan 2
  • Objectives: 10x/0.3, 40x/0.75, 100x/1.4 Oil
  • Fluorescence light source: Lumencor Sola LED
  • Brightfield imaging mode: BF
  • Camera: Hamamatsu C9100-12 EMCCD (512x512, 16um, 16 bits, 31.9 fr/s)
  • XY Stage: Zeiss manual
  • Objective focus: manual
  • Filter wheel: Sutter excitation and emission filter wheels
  • Auto-focusing: N/A
  • Live experiment support: N/A
  • Sample holder: universal slide and dish holder
  • Optical bench: lab bench
  • Commercial dervice contract: N/A
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5 BF DIC
Excitation filter 402/15 (Pos0) 473/28 (Pos8) 555/25 (Pos7) 635/30 (Pos6) Empty
Dichroic (M) 439/507/576/668 (Pos1)
Emission filter (M) 455/50 (Pos8) 525/36 (Pos7) 605/52 (Pos6) 705/72 (Pos5) Empty
Alternative filter cube (S) FT395-LP420 (Pos5) BP470-FT510-BP530 (Pos4) BP546-FT580-LP590 (Pos3)

Condenser setup
Contrast BF DIC
Component Empty (Pos0) IX2-DIC20 (Pos3) IX2-DIC60 (Pos4) IX2-DIC100 (Pos5)
Matched Objective Any 10x, 20x 60x 100x

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • CPU: Intel Xeon E5-2620
    • OS: Windows 10
    • RAM: 16 GB
    • Local data drive: 1 TB
    • Network name: sofia.ad.susx.ac.uk
Images taken from AxioVision
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