Olympus Scan R

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General information

  High-throughput content screening (HCS) microscope based on Olympus inverted IX83 fluorescence microscope with automatic XY stage, motorized focus, hardware autofocusing, and large field of view sCMOS camera.

Application
  • HCS brightfield and multi-channel fluorescence imaging of fixed samples
  • Suitable for slides and well plates, optimized to slides based experiment
  • Automatic multi-channel cell counting and fluorescence level quantification, and results presentation
Accessibility
Microscope details
  • Microscope frame: Olympus IX83
  • Objectives: 10x/0.4 20x/0.45 Ph1, 40x/0.6 Ph2, 40x/0.95 Ph2, 60x/0.7 Ph2, 60x/0.9 Ph2
  • Fluorescence light source: Lumencor Spectra X LED
  • Brightfield imaging mode: BF and PH
  • Camera: Hamamatsu Flash4.0 Lite (sCMOS: 2048x2048, 6.45um, 16 bits, 30 fr/s)
  • XY Stage: Olympus stepper motor stage
  • Auto-focusing: hardware (ZDC) and software based (coarse and fine)
  • Olympus 10-position fast emission filter wheel
  • Live experiment support: dark chamber
  • Sample holder: options of 4-slide and 96 well plate holders
  • Optical bench: lab bench
  • Commercial dervice contract: pay based on engineer visit and broken parts
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5 BF, PH
LED 395/25 470/24 550/15 640/30 Halogen
Excitation filter 395/25 470/30 554/23 640/30 N/A
Dichroic FF409/493/573/652 Dio01 (Pos 2 default)
Emission filter 432/36(Pos 1) 515/30(Pos 2) 595/33(Pos 3) 680/40(Pos 4) Empty(Pos 5)

Alternative setup
Fluorophores CFP YFP HcRed
LED 440/20 510/25 575/25
Excitation filter 434/17 510/10 Empty
Dichroic Empty
Emission filter Empty Empty Empty

Eyepiece filter setup
Fluorophores DAPI/eGFP/TRITC/Cy5 BF, PH
Emission filter FF432/515/595/730 empty

Condenser setup
Contrast Ph1 (Pos1) Ph2 (Pos2) BF (Pos3)
Matched Objective Any 10x ph1 and 20x ph1 40x ph2 and 60x ph2

Workstation and software
  • Acquisition software: ScanR Acquisition V3.0
  • Quantification software: ScanR Analysis V3.0
  • Acquisition workstation (HP Z4G4)
    • CPU: Intel Core i7-7800x 3.60 GHz 6-Core Processor
    • GPU: NVIDIA Quadro P620 2 GB 4mDP GFX Graphics-Board
    • OS: Windows 10
    • RAM: 16 GB
    • Local data drive: 4 TB
  • Analysis workstation
    • Name for booking: Workstation_Madrid
    • Location: JMS 2C41
    • CPU: Intel Xeon E5-1630
    • OS: Windows 10
    • RAM: 16 GB
    • Local data drive: 1.8 TB
    • Network name: osis.ad.susx.ac.uk
    • Offline Scan R Analysis V3.0 with direct access to the data on acquisition workstation
    • Data presentation software: TIBCO Spotfire Analyst for High-Contect Analysis
Images taken from Scan R
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