Leica SP8

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General information

  Leica point scanning confocal with automatic XYZ stage, hybrid detectors, resonant scanner and live experiment support.

Application
  • Multi-channel confocal fluorescence imaging of fixed/live samples
  • Multi-channel video-rate fast confocal aquisition
  • Hyper-spectral fluorescence imaging with spectral unmixing
Accessibility
Microscope details
  • Microscope frame: Leica DMi6000
  • Dual Galvo scanner system
    • XY Galvo-Galvo scanner with adjustable scanning speed(100 - 1600 Hz)
    • XY Resonant-Galvo scanner with 8K Hz scanning speed
  • 4 point detectors
    • 2 PMTs (ch1 and ch3)
    • 2 hybrid detectors (ch2 and ch4)
  • Objectives: 10x/0.4, 20x/0.75, 40x/1.1 W CORR, 63x/1.2 W CORR, 63x/1.4 Oil, 100x/1.44 Oil CORR
  • Adjustable emission spectral windows for all detectors with 1nm resolution
  • Excitation lasers: 405 nm, 458nm, 488nm, 514nm, 561nm, 633 nm
  • Light source for sample inspection: EL6000 metal halide
  • Brightfield imaging mode: BF and DIC
  • Motorised XY Stage: Marzhauser Wetzlar GmbH
  • Motorised Z focus
    • Objective
    • Sample stage (300 um range)
  • Auto-focusing: hardware
  • Live experiment support: heated live chamber (37 deg.) and 5% CO2 (humidified)
  • Sample holder: options of slide, 96-well plate and petridish
  • Optical bench: active
  • Commercial dervice contract: PremiumCare - March 2021
Filter settings
Sample location setup
Fluorophores DAPI GFP Cy3 BF
Filter Cube A
Ex: 360/20, BS: 400, Em: LP425
K3
Ex: 480/20, BS: 510, Em: LP515
Y
Ex: 535/50, BS: 565, Em: 610/75
Empty

Workstation and software
  • Acquisition software: LAS X
  • Acquisition workstation
    • Model: HP Z640
    • CPU: Intel Xeon E5-2650 V3 @2.3GHz
    • OS: 64-bit Windows 10
    • RAM: 32GB
    • Local data drive (D:): 4 TB
    • Network name: leica-sp8pr-ws.ad.susx.ac.uk
Images taken from SP8
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