Zeiss LSM510 Upright

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General information

  Zeiss point scanning confocal based on an upright microscope with motorized focus.

Application
  • Multi-channel confocal fluorescence imaging of fixed samples
Accessibility
Microscope details
  • Microscope frame: Zeiss AxioPlan 2
  • Galvo-Galvo scanner system with adjustable scanning speed(100 - 900 Hz)
  • 3 PMT point detectors
  • Objectives: 5x/0.15, 10x/0.45, 20x/0.8, 40x/0.6, 40x/1.3 Oil, 60x/1.4 Oil
  • Excitation lasers: 458nm, 488nm, 514nm, 543nm, 633 nm
  • Light source for sample location: Zeiss mercury lamp unit
  • Brightfield imaging mode: BF and DIC
  • Manual XY Stage
  • Motorised Z focus unit
  • Auto-focusing: N/A
  • Live experiment support: N/A
  • Sample holder: universal slide and petridish
  • Optical bench: passive
  • Commercial dervice contract: N/A
Filter settings
Sample location setup
Fluorophores DAPI GFP Cy3 DIC BF
Filter Cube Fset 49 wf Fset 38 wf Fset 43 wf Analyser Empty

Workstation and software
  • Acquisition software: LSM
  • Acquisition Workstation
    • CPU: Intel Xeon E5-2637 @3.5GHz (2 processors)
    • OS: Windows NT
    • RAM: 96GB
    • Local data drive: 2 TB
    • No network connection
Images taken from LSM510 upright
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