Olympus IX73

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General information

  Olympus inverted microscope with automatic XY stage, large field sCOMS camera, motorized focus and live experiment support.

Application
  • Brightfield and multi-channel fluorescence imaging of fixed/live samples
  • Optimized to live experiments
Accessibility
Microscope details
  • Microscope frame: Olympus IX73
  • Objectives: 20x/0.45, 40x/0.6, 100x/1.4 Oil
  • Fluorescence light source: Coolled pE-4000
  • Brightfield imaging mode: BF and DIC
  • Camera: Photometrics Prime (sCOMS: 2048x2048, 6.45um, 16 bits, 30 fr/s)
  • XY Stage: ASI stepper motor stage
  • Z stage: Prior stepper motor focus drive PS3H122R_NB
  • Prior 10-position emission filter wheel
  • Auto-focusing: software based
  • Live experiment support: heated live chamber (37 deg.) and 5% CO2 (humidified)
  • Sample holder: options of slide, dish and well plates
  • Optical bench: passive
  • Commercial dervice contract: annual microscope basic service in November
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5 SIR 700 BF DIC
LED 365 490 550 635 White LED
Excitation filter 395/25 470/20 550/15 640/30 N/A
Filter Cube 409/493/573/652 (Pos8 default)
Emission filter 435/40 (Pos7) 525/50 (Pos8) 617/73 (Pos10) 680/40 (Pos5) 740/60(Pos6) Empty(Pos2) Analyser (Pos1)

Alternative setup
Fluorophores CFP YFP CFP/GFP/Cy3 GFP/HcRed DAPI/GFP/Cy3/Cy5
LED 435 500 435/490/555/635 490/595 365/490/550/635
Excitation filter 434/17 510/10 434/470/550/640 470/590 435/525/617/680
Dichroic cube 444/520/590 (Pos1) 520/633 (Pos3) 410/515/582/669
Emission filter 480/17 (Pos3) 537/26 (Pos4) 480/525/617 (seq.) 525/? (seq.) 435/525/617/680 (seq.)

Eyepiece filter setup
Fluorophores DAPI/eGFP/TRITC/Cy5 eCFP Cy3 eGFP/MCherry DIC
Emission filter 432/515/595/730 (Pos2 green, default) 457/50(Pos1, blue) 607/36 (Pos3 Red) ?(Pos4) Analyzer(Pos5)

Condenser setup
Contrast PH1 BF DIC
Component Ph1 ring (Pos1) Empty (Pos2) IX2-DIC20 (Pos3) IX2-DIC40 (Pos4) IX2-DIC60 (Pos5)
Matched Objective 10x ph1 and 20x ph1 Any 10x, 20x 40x 60x and 100x

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • CPU: Intel Xeon E5-2620
    • OS: Windows 10
    • RAM: 16 GB
    • Local data drive: 1 TB
    • Network name: ix73.ad.susx.ac.uk
Images taken from IX73
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