Olympus IX71

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General information

  Olympus inverted microscope with automatic XY stage, motorized focus, large field of view sCOMS camera and live experiment support.

  • Multi-channel brightfield and fluorescence imaging of fixed/live samples
  • Optimized to live experiments
Microscope details
  • Microscope frame: Olympus IX71
  • Objectives: 10x/0.3, 20x/0.45, 40x/0.95, 60x/1.4 Oil, 100x/1.4 Oil
  • Fluorescence light source: Coolled pE-4000
  • Brightfield imaging mode: BF and DIC
  • Camera: Hamamatsu Flash4.0 Lite (2048x2048 pixels, 6.45um, 16 bits, 30 fr/s)
  • XY Stage: Prior stepper motor stage H117E2XD/B
  • Objective focus: Prior stepper motor focus drive PS3H122R_NB
  • Prior 10-position emission filter wheel
  • Auto-focusing: software based
  • Live experiment support: heated live chamber (37 deg.) and 5% CO2 (humidified)
  • Sample holder: options of slide, dish and well plates
  • Optical bench: passive
  • Commercial dervice contract: annual microscope basic service in November
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5 BF DIC
LED 365 490 550 635 White LED
Excitation filter 395/25 470/24 550/15 640/40 N/A
Dichroic cube 439/507/576/668 (Pos1 default)
Emission filter 455/50 (Pos5) 525/36 (Pos6) 617/73 (Pos7) 705/72 (Pos8) Empty Analyser (Pos3)

Alternative setup
Fluorophores CFP YFP RFP GFP/HcRed
LED 435 500 595 490/595
Excitation filter New excitation filters required
Dichroic 469/535/637(Pos1) FF527/645
Emission filter FF464/547 (Pos2) AT610LP (Pos6)

Eyepiece filter setup
Emission filter 444/520/595/703 (Pos0) 469/535/637(Pos1) Analyzer (Pos2) Empty (Pos3)

Condenser setup
Contrast BF DIC
Component Empty (Pos0) IX2-DIC20 (Pos3) IX2-DIC60 (Pos4) IX2-DIC100 (Pos5)
Matched objective Any 10x, 20x 60x 100x

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • CPU: Intel Xeon E3-1230V2 @3.3GHz
    • OS: 64-bit Windows 10
    • RAM: 8 GB
    • Local data drive (D): 2 TB
    • Network name: cellvivo.ad.susx.ac.uk
Images taken from IX71
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