Olympus IX70

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General information

  Olympus inverted microscope with automatic XY stage and motorised focusing.

Application
  • Multi-channel fluorescence imaging of fixed samples
Accessibility
Microscope details
  • Microscope frame: Olympus IX70
  • Objectives: 40x/1.35 Oil, 60x/1.42 Oil
  • Fluorescence light source:  Lumen 200S/220S metal halide system
  • Brightfield imaging mode: N/A
  • Camera: Hamamatsu ORCA ER CCD (1344X1024 pixel, 12-bit, 8.3 fr/s)
  • XY Stage: Prior stepper motor stage H117N21X/G
  • Objective focus: Prior stepper motor focus drive PS3H122R_NB
  • Prior excitation and emission filter wheels
  • Prior excitation shutter
  • Auto-focusing: software based
  • Live experiment support: N/A
  • Sample holder: universal slide/dish
  • Optical bench: passive
  • Commercial dervice contract: annual microscope basic service in November
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5
Excitation filter 387/11 (Pos4) 485/20 (Pos5) 560/25 (Pos6) 650/13 (pos7)
Dichroic cube 410/504/582/669(Pos4 default)
Emission filter 440/40 (Pos4) 520/36 (Pos5) 607/36 (Pos6) 684/24 (Pos7)
Eyepiece filter 444/520/595/703 (Pos2)

Alternative imaging setup
Fluorophores CFP/YFP/RFP/Cy5 BFP/CFP/YFP/RFP/Cy5 CFP/TRITC
Filter cube 453/522/600/688 (Pos1) 432/465/522/600/679 (Pos2) 469/555 (Pos3)

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • CPU: Intel Core i7-2600 @3.4GHz
    • OS: 64-bit Windows 10
    • RAM: 16 GB
    • Local data drive (D): 1 TB
    • Network name: GC01009660.ad.susx.ac.uk
Images taken from IX70
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