Zeiss AxioVert

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General information

  Zeiss inverted microscope with automatic XY stage, motorised focusing and live experiment support.

Application
  • Brightfield and multi-channel fluorescence imaging of fixed/live samples
Accessibility
Microscope details
  • Microscope frame: Zeiss AxioVert 200M
  • Objectives: 10x/0.3, 20x/0.45, 40x/0.75, 60x/1.4 Oil, 100x/1.4 Oil
  • Fluorescence light source: Zeiss HXP 120C metal halide
  • Brightfield imaging mode: BF and DIC
  • Camera: Hamamatsu ORCA ER (1344X1024, 6.45um, 12-bit, 8.3 fr/s)
  • XY Stage: Zeiss stepper motor stage
  • Axial focus: motorized objective drive
  • Auto-focusing: software based
  • Live experiment support: heated live chamber (37 deg.)
  • Sample holder: options of slide, dish and well plates
  • Optical bench: lab bench
  • Commercial dervice contract: N/A
Filter settings
Default setup
Fluorophores DAPI GFP Cy3 Cy5 BF DIC
Filter Cube Set01
Ex: BP365/12
BS: FT395
Em: LP397
Set10
Ex: BP450-490
BS: FT510
Em: BP515-565
Set15
Ex: BP546/12
BS: FT580
Em: LP590
Set26
Ex: BP575-625
BS: FT645
Em: BP660-710
Halogen
Cub Position Pos1 Pos2 Pos3 Pos4 Pos5

Condenser setup
Contrast BF Phase Contrast DIC
Component H (Pos1) Ph1 (Pos2) Ph2 (Pos3) Ph3 (Pos4) DIC II (Pos5) DIC III (Pos6)
Matched Objective 10x ph1 and 20x ph1 Any 10x, 20x 40x 60x and 100x

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • Dell Precision T1700
    • CPU: Intel Core i5-4570 @3.2GHz
    • OS: 64-bit Windows 10
    • RAM: 8 GB
    • Local data drive E: 300GB
    • Network name: gc01007444.ad.susx.ac.uk
Images taken from AxioVert
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