Zeiss AxioPhot

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General information

  Zeiss upright microscope with manual XY stage and manual focusing.

Application
  • 2D brightfield and 4-channel fluorescence for fixed/live cell inspection
  • 2D color absorption imaging
Accessibility
Microscope details
  • Microscope frame: Zeiss AxioPhot
  • Objectives: 5x/0.15, 10x/0.3, 40x/0.75, 100x/1.3 Oil
  • Fluorescence light source: Zeiss mercury lamp
  • Brightfield imaging mode: BF, PH
  • Camera: Zeiss CCD AxioCam HRc
    • Monochrome mode: 1300x1030, 6.7um, 14bits, 5fr/s
    • Color mode: 432X342, 6.7um, 13fr/s
  • Manual XY Stage
  • Manual objective focus
  • Manual filter cube switch
  • Live experiment support: N/A
  • Sample holder: universal slide/dish
  • Optical bench: lab bench
  • Commercial dervice contract: N/A
Filter settings
Imaging setup
Fluorophores DAPI GFP Cy3 BF
Filter Cube Ex: G365
BS: FT395
Em: LP420
Ex: 450-490
BS: FT510
Em: 515-565
Ex: G546
BS: FT580
Em: LP590)
Take the filter unit out
Filter Position I II III N/A

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • Model: Dell OptiPlex 780
    • CPU: Intel Core 2 Duo CPU E7500 @2.93GHz
    • OS: 64-bit Windows 10
    • RAM: 12 GB
    • Local data drive: On C:
    • Network name: gdsc23007271.ad.susx.ac.uk
Images taken from AxioPhot
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