Role

Senior Research Fellow - Cancer Research UK DNA Repair Enzymes Group

Laboratory Homepage: http://www.sussex.ac.uk/lifesci/oliverlab

The Cancer Research UK - DNA Repair Enzymes Group (PIs Dr. Antony Oliver and Prof. Laurence Pearl) principally uses the technique of X-ray Crystallography to investigate and understand the molecular mechanisms underpinning the process of DNA damage detection and repair in cells.  We complement this with a wide range of other experimental techniques, including Biophysics, Biochemistry and Bioinformatics. We have particular expertise in protein expression (in E.coli and insect cells) and purification, along with spectroscopic techniques (CD and fluorescence).

Lecturer

Final year module, Protein Form and Function - C7124
(Chromatography and Protein Purification)

Undergraduate Project Supervisor

Final year, laboratory and literature (critical review)-based projects

PhD Supervisor

Supervision of post-graduate students, undertaking research-based doctoral programmes

Silva-Santisteban, M Cris, Westwood, Isaac M, Boxall, Kathy, Brown, Nathan, Peacock, Sam, McAndrew, Craig, Barrie, Elaine, Richards, Meirion, Mirza, Amin, Oliver, Antony W, Burke, Rosemary, Hoelder, Swen, Jones, Keith, Aherne, G Wynne, Blagg, Julian, Collins, Ian, Garrett, Michelle D and van Montfort, Rob L M (2013) Fragment-based screening maps inhibitor interactions in the ATP-binding site of checkpoint kinase 2. PLoS ONE, 8 (6). e65689. ISSN 1932-6203

Grundy, Gabrielle L, Rulten, Stuart L, Zeng, Zhihong, Arribas-Bosacoma, Raquel, Iles, Natasha, Manley, Katie, Oliver, Antony and Caldecott, Keith W (2013) APLF promotes the assembly and activity of non-homologous end joining protein complexes. The EMBO journal, 32 (1). pp. 112-125. ISSN 1460-2075

Brownlee, Peter M, Chambers, Anna L, Oliver, Antony W and Downs, Jessica A (2012) Cancer and the bromodomains of BAF180. Biochemical Society Transactions, 40 (2). pp. 364-369. ISSN 0300-5127

Ali, Ammar A E, Timinszky, Gyula, Arribas-Bosacoma, Raquel, Kozlowski, Marek, Hassa, Paul O, Hassler, Markus, Ladurner, Andreas G, Pearl, Laurence H and Oliver, Antony W (2012) The zinc-finger domains of PARP1 cooperate to recognize DNA strand breaks. Nature Structural & Molecular Biology, 9 (7). pp. 685-692. ISSN 1545-9985

Garces, Fernando, Pearl, Laurence H. and Oliver, Antony W. (2011) The structural basis for substrate recognition by mammalian polynucleotide kinase 3' phosphatase. Molecular Cell, 44 (3). 385 - 396. ISSN 1097-2765

Boos, Dominik, Sanchez-Pulido, Luis, Rappas, Mathieu, Pearl, Laurence H, Oliver, Antony W, Ponting, Chris P and Diffley, John F X (2011) Regulation of DNA replication through Sld3-Dpb11 interaction is conserved from yeast to humans. Current Biology, 21 (13). pp. 1152-1157. ISSN 0960-9822

Caldwell, John J, Welsh, Emma J, Matjissen, Cornelis, Anderson, Victoria E, Antoni, Laurent, Boxall, Kathy, Urban, Frederique, Hayes, Angela, Raynaud, Florence I, Rigoreau, Laurent J M, Raynham, Tony, Aherne, G Wynne, Pearl, Laurence H, Oliver, Antony W, Garrett, Michelle D and Collins, Ian (2011) Structure-Based Design of Potent and Selective 2-(Quinazolin-2-yl)phenol Inhibitors of Checkpoint Kinase 2. Journal of Medicinal Chemistry, 54 (2). pp. 580-590. ISSN 0022-2623

Anderson, Victoria E, Walton, Michael I, Eve, Paul D, Boxall, Katherine J, Antoni, Laurent, Caldwell, John J, Aherne, Wynne, Pearl, Laurence H, Oliver, Antony W, Collins, Ian and Garrett, Michelle D (2011) CCT241533 Is a Potent and Selective Inhibitor of CHK2 that Potentiates the Cytotoxicity of PARP Inhibitors. Cancer Research, 71 (2). pp. 463-472. ISSN 0008-5472

Rappas, Mathieu, Oliver, Antony W and Pearl, Laurence H (2011) Structure and function of the Rad9-binding region of the DNA-damage checkpoint adaptor TopBP1. Nucleic Acids Research, 39 (1). pp. 313-324. ISSN 0305-1048

Woodbine, Lisa, Grigoriadoub, Sofia, Goodarzi, Aaron A, Riballo, Enriqueta, Tape, Christopher, Oliver, Antony W, van Zelm, Menno C, Buckland, Matthew S, Davies, E Graham, Pearl, Laurence H and Jeggo, Penny A (2010) An Artemis polymorphic variant reduces Artemis activity and confers cellular radiosensitivity. DNA Repair, 9 (9). pp. 1003-1010. ISSN 1568-7864

Hilton, Stephen, Naud, Sebastien, Caldwell, John J, Boxall, Kathy, Burns, Samantha, Anderson, Victoria E, Antoni, Laurent, Allen, Charlotte E, Pearl, Laurence H, Oliver, Antony W, Aherne, G Wynne, Garrett, Michelle D and Collins, Ian (2010) Identification and characterisation of 2-aminopyridine inhibitors of checkpoint kinase 2. Bioorganic & Medicinal Chemistry, 18 (2). pp. 707-718. ISSN 0968-0896

Oliver, Antony W, Swift, Sally, Lord, Christopher J, Ashworth, Alan and Pearl, Laurence H (2009) Structural basis for recruitment of BRCA2 by PALB2. EMBO Reports, 10 (9). pp. 990-996. ISSN 1469-221X

Ali, Ammar A. E., Jukes, Rachel M., Pearl, Laurence H. and Oliver, Antony W. (2009) Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK. Nucleic Acids Research, 37 (5). pp. 1701-1712. ISSN 0305-1048

Cancer Research Technology Ltd; Collins, Ian; Caldwell, John J; Oliver, Antony W; Raynham, Tony M; Welsh, Emma J; Matijssen, Cornelius A J (2008) Therapeutic oxy-phenyl-aryl compounds and their use. PCT/GB2008/003586.

Pike, Ashley C W, Rellos, Peter, Niesen, Frank H, Turnbull, Andrew, Oliver, Antony W, Parker, Sirlester A, Turk, Benjamin E, Pearl, Laurence H and Knapp, Stefan (2008) Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites. EMBO Journal, 27 (4). pp. 704-714. ISSN 0261-4189

Oliver, Antony W, Knapp, Stefan and Pearl, Laurence H (2007) Activation segment exchange: a common mechanism of kinase autophosphorylation? Trends in Biochemical Science, 32 (8). pp. 351-356.

Dore, A S, Kilkenny, M L, Jones, S A, Oliver, A W, Roe, S M, Bell, S D and Pearl, L H (2006) Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity. Nucleic Acids Research, 34 (16). pp. 4515-4526.

Oliver, Antony W, Paul, Angela, Boxall, Katherine J, Barrie, S Elaine, Aherne, G Wynne, Garrett, Michelle D, Mittnacht, Sibylle and Pearl, Laurence H (2006) Trans-activation of the DNA-damage signalling protein kinase Chk2 by T-loop exchange. EMBO Journal, 25 (13). pp. 3179-3190. ISSN 0261-4189

Oliver, Antony W, Jones, Sarah A, Roe, Stephen Mark, Matthews, Steve, Goodwin, Graham H and Pearl, Laurence H (2005) Crystal structure of the proximal BAH domain of the polybromo protein. Biochemical Journal, 389 (3). pp. 657-664. ISSN 0264-6021

Oliver, A W, Amé, J C, Roe, S M, Good, V, de Murcia, G and Pearl, L H (2004) Crystal structure of the catalytic fragment of murine poly(ADP-ribose) polymerase-2. Nucleic Acids Research, 32 (2). pp. 456-464. ISSN 0305-1048

Oliver, Antony, Carpenter, ML and Kneale, GG (2001) Analysis of DNA-protein interactions by intrinsic fluorescence. In: Methods in Molecular Biology. Unset, pp. 491-502.

Carpenter, M L, Oliver, A W and Kneale, G G (2001) Circular dichroism for the analysis of protein-DNA interactions. In: Methods in Molecular Biology. Unset, pp. 503-510.

Oliver, Antony W, Bogdarina, Irina, Schroeder, Ewald, Taylor, Ian A and Kneale, G Geoff (2000) Preferential binding of fd gene 5 protein to tetraplex nucleic acid structures. Journal of Molecular Biology, 301 (3). pp. 575-584.

Oliver, Antony W and Kneale, G Geoff (1999) Structural characterization of DNA and RNA sequences recognized by the gene 5 protein of bacteriophage fd. Biochemical Journal, 339 (3). pp. 525-531.

Oliver, Antony W and Kneale, G Geoff (1997) Preferential binding of the fd gene 5 protein to a structured form of the single-stranded DNA sequence d(GT5G4CT4C). Biochemical Society Transactions, 25 (4). S643.