Nikon E400

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General information

  Nikon upright microscope with manual XY stage and manual focusing.

Application
  • 2D brightfield and 4-channel fluorescence for fixed/live cell inspection and imaging
Accessibility
Microscope details
  • Microscope frame: Nikon E400
  • Objectives: 4x/0.1, 10x/0.25, 40x/0.65, 100x/1.3 Oil
  • Fluorescence light source: pE-300 white
  • Brightfield imaging mode: BF
  • Camera: Hamamatsu CCD ORCA-05G (1344x1024, 6.45um, 12 bits, 8.9 fr/s)
  • Manual XY Stage
  • Manual objective focus
  • Manual filter cube change
  • Live experiment support: N/A
  • Sample holder: universal slide
  • Optical bench: lab bench
  • Commercial dervice contract: annual microscope basic service in November
Filter settings
Sample inspection setup
Fluorophores DAPI GFP Cy3 Cy5
LED 400 450 550 640
Filter Cube UV-2A
Ex:330-380
BS:DM400
Em:BA420
Pos 1
B-2A
Ex:450-490
BS:DM505
Em:BA520
Pos 2
G-2A
Ex:510-560
BS:DM575
Em:BA590
Pos 3
ET-Cy5
Ex:620/60
BS:DM660
Em:700/75
Pos 4

Workstation and software
  • Acquisition software: Micro-manager
  • Acquisition workstation
    • CPU: Intel Core i5-4570 @ 3.2GHz
    • OS: 64-bit Windows 10
    • RAM: 8 GB
    • Local data drive: E: 1TB
    • Network name: zeissupright.ad.susx.ac.uk
Images taken from E400
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