When Outlook opens, and the reading pane is showing, the most recent message is displayed in the preview. When you click onto another message, the previous one may change colour and be marked as read. The same thing may happen if you preview any other message by clicking on it.
To stop this happening in Outlook 2010 and Outlook 2013, follow these instructions:
Note that the menu appearance differs slightly between Outlook 2010 and 2013, but the method is the same.
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This is question number 2285, which appears in the following categories: