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Publication Type J
Authors Shih, YJ; Chang, HC; Tsai, MC; Wu, TY; Wu, TC; Kao, P; Kao, WY; Chang, IF
Author Full Name Shih, Yun-Jhih; Chang, Hui-Chun; Tsai, Min-Chieh; Wu, Ting-Ying; Wu, Tai-Chung; Kao, Ping; Kao, Wen-Yuan; Chang, Ing-Feng
Title Comparative leaf proteomic profiling of salt-treated natural variants of Imperata cylindrica
Source TAIWANIA
Language English
Document Type Article
Keywords Plus DIFFERENCE GEL-ELECTROPHORESIS; STRESS-RESPONSIVE PROTEINS; ECOTYPIC VARIATION; GENE-EXPRESSION; SALINITY; IDENTIFICATION; LEAVES; POPULATIONS; MECHANISMS; TAIWAN
Abstract Cogon grass (Imperata cylindrica (L.) Beauv. var. major (Nees) Hubb.) is one of the top-ten weeds worldwide. It is also a C-4 medicinal plant. In particular, an ecotype from Chuwei (CW) mangrove forest was found to be salt tolerant. Comparative proteomic analysis using two-dimensional (2D)-difference in gel electrophoresis coupled with liquid chromatography-mass spectrometry (LC-MS) was carried out to identify responsive leaf proteins in the CW ecotype and salt-intolerant Sarlun (SL) population following three days of 150 mM sodium chloride salt stress treatment. We identified five photosynthesis proteins including Rubisco small subunit, uncharacterized protein LOC100194054, Cyt b6-f, oxygen-evolving enhancer 2, and photosystem I reaction center subunit IV which were significantly up- or down-regulated by salt stress in CW ecotype but not SL population. Gene ontology enrichment analysis showed that photosynthesis was over-represented. The mass spectrometry proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD008482. Taken together, our proteomic study identified differentially accumulated proteins which provide additional evidence of ecophysiological variation in two natural variants of I. cylindrica.
Author Address [Shih, Yun-Jhih; Chang, Hui-Chun; Tsai, Min-Chieh; Chang, Ing-Feng] Natl Taiwan Univ, Inst Plant Biol, Taipei, Taiwan; [Wu, Ting-Ying; Kao, Ping; Chang, Ing-Feng] Natl Taiwan Univ, Dept Life Sci, Taipei, Taiwan; [Wu, Tai-Chung; Kao, Wen-Yuan] Natl Taiwan Univ, Inst Ecol & Evolut Biol, Taipei, Taiwan; [Chang, Ing-Feng] Natl Taiwan Univ, Genome & Syst Biol Degree Program, Taipei, Taiwan; [Chang, Ing-Feng] Acad Sinica, Taipei, Taiwan
Reprint Address Chang, IF (reprint author), Natl Taiwan Univ, Inst Plant Biol, Taipei, Taiwan.; Chang, IF (reprint author), Natl Taiwan Univ, Dept Life Sci, Taipei, Taiwan.; Chang, IF (reprint author), Natl Taiwan Univ, Genome & Syst Biol Degree Program, Taipei, Taiwan.; Chang, IF (reprint author), Acad Sinica, Taipei, Taiwan.
E-mail Address ifchang@ntu.edu.tw
Funding Agency and Grant Number Ministry of Science and Technology, Taiwan [104-2311-B-002-034, 106-2311-B-002-014, 106-2313-B-002-004]; National Taiwan University [105R892002]
Funding Text We appreciate funding support from the Ministry of Science and Technology, Taiwan (MOST# 104-2311-B-002-034, MOST# 106-2311-B-002-014, and MOST# 106-2313-B-002-004) and the National Taiwan University (Grant # 105R892002). We thank Mr. C.S. Wang and Mr. C.H. Wang (Tamsui Farm) for help with Imperata cylindrica sampling. Many thanks to BioMed Proofreading, LLC for English editing. We appreciate the technical support from Visual Protein,Taipei, Taiwan. We also thank Technology Commons, College of Life Science, National Taiwan University, for technical help.
Cited Reference Count 62
Publisher NATL TAIWAN UNIV PRESS
Publisher City TAIPEI
Publisher Address NO 1 SECTION 4, ROOSEVELT RD, TAIPEI, 106, TAIWAN
ISSN 0372-333X
29-Character Source Abbreviation TAIWANIA
ISO Source Abbreviation Taiwania
Publication Date JUN
Year Published 2018
Volume 63
Issue 2
Beginning Page 171
Ending Page 182
Digital Object Identifier (DOI) 10.6165/tai.2018.63.171
Page Count 12
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number GM3JO
Unique Article Identifier WOS:000438001100011
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