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Publication Type J
Authors Solis, J; Baisakh, N; Brandt, SR; Villordon, A; La Bonte, D
Author Full Name Solis, Julio; Baisakh, Niranjan; Brandt, Steven R.; Villordon, Arthur; La Bonte, Don
Title Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato
Source PLOS ONE
Language English
Document Type Article
Keywords Plus VACUOLAR H+-PYROPHOSPHATASE; OVERLY SENSITIVE PATHWAY; ENHANCES SALT TOLERANCE; ARABIDOPSIS-THALIANA; NA+/H+ ANTIPORTER; STRESS TOLERANCE; FACTOR GENE; ROOT TRANSCRIPTOME; FIELD CONDITIONS; PROTEIN-KINASES
Abstract The response and adaption to salt remains poorly understood for beach morning glory [Ipomoea imperati (Vahl) Griseb], one of a few relatives of sweetpotato, known to thrive under salty and extreme drought conditions. In order to understand the genetic mechanisms underlying salt tolerance of a Convolvulaceae member, a genome-wide transcriptome study was carried out in beach morning glory by 454 pyrosequencing. A total of 286,584 filtered reads from both salt stressed and unstressed (control) root and shoot tissues were assembled into 95,790 unigenes with an average length of 667 base pairs (bp) and N50 of 706 bp. Putative differentially expressed genes (DEGs) were identified as transcripts over-represented under salt stressed tissues compared to the control, and were placed into metabolic pathways. Most of these DEGs were involved in stress response, membrane transport, signal transduction, transcription activity and other cellular and molecular processes. We further analyzed the gene expression of 14 candidate genes of interest for salt tolerance through quantitative reverse transcription PCR (qRT-PCR) and confirmed their differential expression under salt stress in both beach morning glory and sweetpotato. The results comparing transcripts of I. imperati against the transcriptome of other Ipomoea species, including sweetpotato are also presented in this study. In addition, 6,233 SSR markers were identified, and an in silico analysis predicted that 434 primer pairs out of 4,897 target an identifiable homologous sequence in other Ipomoea transcriptomes, including sweetpotato. The data generated in this study will help in understanding the basics of salt tolerance of beach morning glory and the SSR resources generated will be useful for comparative genomics studies and further enhance the path to the marker-assisted breeding of sweetpotato for salt tolerance.
Author Address [Solis, Julio; Baisakh, Niranjan; La Bonte, Don] Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA; [Brandt, Steven R.] Louisiana State Univ, Louisiana Digital Media Ctr, Baton Rouge, LA 70803 USA; [Villordon, Arthur] Louisiana State Univ, Ctr Agr, Sweet Potato Res Stn, Chase, LA USA
Reprint Address La Bonte, D (reprint author), Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA.
E-mail Address nbaisakh@agcenter.lsu.edu; dlabonte@agcenter.Isu.edu
ORCID Number SOLIS SARMIENTO, JULIO/0000-0001-5183-6332; Brandt, Steven/0000-0002-7979-2906
Funding Agency and Grant Number HATCH grant from the USDA-NIFA
Funding Text The study was supported by a HATCH grant from the USDA-NIFA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Cited Reference Count 75
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher PUBLIC LIBRARY SCIENCE
Publisher City SAN FRANCISCO
Publisher Address 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
ISSN 1932-6203
29-Character Source Abbreviation PLOS ONE
ISO Source Abbreviation PLoS One
Publication Date FEB 5
Year Published 2016
Volume 11
Issue 2
Article Number e0147398
Digital Object Identifier (DOI) 10.1371/journal.pone.0147398
Page Count 19
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number DC9PR
Unique Article Identifier WOS:000369554000015
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