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Publication Type J
Authors Chen, TX; Johnson, R; Chen, SH; Lv, H; Zhou, JL; Li, CJ
Author Full Name Chen, Taixiang; Johnson, Richard; Chen, Shuihong; Lv, Hui; Zhou, Jingle; Li, Chunjie
Title Infection by the fungal endophyte EpichloA << bromicola enhances the tolerance of wild barley (Hordeum brevisubulatum) to salt and alkali stresses
Source PLANT AND SOIL
Language English
Document Type Article
Author Keywords Epichloe endophyte; Hordeum brevisubulatum; Salt stress; Alkali stress
Keywords Plus WATER-STRESS; PROLINE ACCUMULATION; ADAPTIVE STRATEGIES; SALINITY TOLERANCE; OSMOTIC ADJUSTMENT; POWDERY MILDEW; ABIOTIC STRESS; IONIC BALANCE; PLANTS; GROWTH
Abstract Salinization is considered as a major environmental threat to agricultural systems. Infection with EpichloA << fungal endophytes has been shown to increase tolerance to NaCl stress for several host grass species, but limited information is available regarding the effects of these endophytes under mixed salt (NaCl and Na2SO4) and mixed alkali (NaHCO3 and Na2CO3) stresses. Since these four compounds are considered very harmful to many inland areas in China, we conducted a study to determine the impact of EpichloA << fungal endophyte infection on wild barley (Hordeum brevisubulatum) under both salt stress (SS) and alkali stress (AS). Wild barley with (E+) and without (E-) EpichloA << endophyte was subjected to mixed salt (molar ratio of NaCl:Na2SO4 = 1:1) and mixed alkali (molar ratio of NaHCO3:Na2CO3 = 1:1) treatments (0, 100, 200, 300 and 400 mM). Photosynthetic parameters and chlorophyll content were measured after 21 days exposure to stress, and growth parameters, physiological indexes, sodium (Na+), potassium (K+), calcium (Ca2+), nitrogen (N), phosphorus (P) and carbon (C) contents were determined after 22 days exposure to stress. The harmful effect of alkali stress on the growth of wild barley was stronger than those of salt stress, irrespective of endophyte infection. Alkali stress had a greater impact on photosynthesis and chlorophyll content compared to salt stress and also accumulated more of the osmoprotectant glycine betaine. However, salt stress appeared to increase total antioxidant capacity as well as increasing K+ content (resulting in a relative low Na+/K+ ratio). Under alkali stress, Ca2+ content sharply increased in roots as opposed to a decrease under salt stress. In roots, the C, N, P contents and the C:N ratio was higher under salt stress compared to alkali stress whereas the C:P and N:P ratios were lower. In shoots, the N and P contents were higher under salt stress compared to alkali stress whereas the C content and the C:P and C:N ratios were lower. Interestingly, the presence of EpichloA << endophyte infection on wild barley under both salt stress and alkali stress led to significant amelioration of both stresses. EpichloA << infection significantly increased photosynthesis, chlorophyll content, total antioxidant capacity and glycine betaine content, whilst lowering leaf malondialdehyde content. Furthermore, EpichloA << infection reduced Na+ content, the Na+/K+ ratio and shoot Ca2+ content but increased K+ content and the root Ca2+ content. EpichloA << infected plants also had higher C, N and P contents but lower ratios of C:N, C:P and N:P than uninfected plants. The presence of the EpichloA << endophyte suppresses the negative effect of salt stress and alkali stress on wild barley seedling growth. The possible mechanisms by which the presence of EpichloA << endophyte enhances growth of plants exposed to those two stresses include improved photosynthetic ability, increased antioxidant potential, increased nutrient absorption, and osmotic and ionic adjustment. The study also found that alkali stress is more harmful to wild barley than salt stress.
Author Address [Chen, Taixiang; Chen, Shuihong; Lv, Hui; Zhou, Jingle; Li, Chunjie] Lanzhou Univ, State Key Lab Grassland Agoecosyst, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs,Coll Pastoral Agr Sci, Lanzhou 730020, Gansu, Peoples R China; [Johnson, Richard] AgResearch Ltd, Grasslands Res Ctr, Private Bag 11-008, Palmerston North 4442, New Zealand
Reprint Address Li, CJ (reprint author), Lanzhou Univ, State Key Lab Grassland Agoecosyst, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs,Coll Pastoral Agr Sci, Lanzhou 730020, Gansu, Peoples R China.
E-mail Address chunjie@lzu.edu.cn
ResearcherID Number Li, Chunjie/G-4988-2012
ORCID Number Li, Chunjie/0000-0002-3287-2140; Johnson, Richard/0000-0003-2100-6658
Funding Agency and Grant Number National Basic Research Program of China [2014CB138702]; Natural Science Foundation of China [31372366]; Program for Changjiang Scholars and Innovative Research Team in University, China [IRT17R50]; Fundamental Research Funds for the Central Universities [LZUJBKY-2016-180, 2017-kb10, 2018-kb10]; 111 Project [B12002]
Funding Text The authors thank Wei Tang, Xiang Yao, Jianfeng Wang, and Yane Guo for assistance with experiments, and Professor Kari Saikkonen and Dr. Michael Christensen for helpful comments and revision of the manuscript. This study was supported by the National Basic Research Program of China (2014CB138702), the Natural Science Foundation of China (31372366), Program for Changjiang Scholars and Innovative Research Team in University, China (IRT17R50), Fundamental Research Funds for the Central Universities (LZUJBKY-2016-180, 2017-kb10, 2018-kb10) and 111 Project (B12002).
Cited Reference Count 73
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 1
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0032-079X
29-Character Source Abbreviation PLANT SOIL
ISO Source Abbreviation Plant Soil
Publication Date JUL
Year Published 2018
Volume 428
Issue 1-2
Beginning Page 353
Ending Page 370
Digital Object Identifier (DOI) 10.1007/s11104-018-3643-4
Page Count 18
Web of Science Category Agronomy; Plant Sciences; Soil Science
Subject Category Agriculture; Plant Sciences
Document Delivery Number GK4KX
Unique Article Identifier WOS:000436129500023
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