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Publication Type J
Authors Crump, BC; Koch, EW
Author Full Name Crump, Byron C.; Koch, Evamaria W.
Title Attached bacterial populations shared by four species of aquatic angiosperms
Source APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Language English
Document Type Article
Keywords Plus GROWTH-PROMOTING RHIZOBACTERIA; SEAGRASS HALOPHILA-STIPULACEA; SULFATE-REDUCING BACTERIA; HALODULE-WRIGHTII ASCHERS; EELGRASS ZOSTERA-MARINA; RIBOSOMAL-RNA ANALYSIS; PLANT-GROWTH; THALASSIA-TESTUDINUM; NITROGEN-FIXATION; SALT-MARSH
Abstract Symbiotic relationships between microbes and plants are common and well studied in terrestrial ecosystems, but little is known about such relationships in aquatic environments. We compared the phylogenetic diversities of leaf- and root-attached bacteria from four species of aquatic angiosperms using denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of PCR-amplified 16S rRNA genes. Plants were collected from three beds in Chesapeake Bay at sites characterized as freshwater (Vallisneria americana), brackish (Potomogeton perfoliatus and Stuckenia pectinata), and marine (Zostera marina). DGGE analyses showed that bacterial communities were very similar for replicate samples of leaves from canopy-forming plants S. pectinata and P. perfoliatus and less similar for replicate samples of leaves from meadow-forming plants Z. marina and V. americana and of roots of all species. In contrast, bacterial communities differed greatly among plant species and between leaves and roots. DNA sequencing identified 154 bacterial phylotypes, most of which were restricted to single plant species. However, 12 phylotypes were found on more than one plant species, and several of these phylotypes were abundant in clone libraries and represented the darkest bands in DGGE banding patterns. Root-attached phylotypes included relatives of sulfur-oxidizing Gammaproteobacteria and sulfate-reducing Deltaproteobacteria. Leaf-attached phylotypes included relatives of polymer-degrading Bacteroidetes and phototrophic Alphaproteobacteria. Also, leaves and roots of three plant species hosted relatives of methylotrophic Betaproteobacteria belonging to the family Methylophilaceae. These results suggest that aquatic angiosperms host specialized communities of bacteria on their surfaces, including several broadly distributed and potentially mutualistic bacterial populations.
Author Address [Crump, Byron C.; Koch, Evamaria W.] Univ Maryland, Ctr Environm Sci, Horn Point Lab, Cambridge, MD 21613 USA
Reprint Address Crump, BC (reprint author), Univ Maryland, Ctr Environm Sci, Horn Point Lab, 2020 Horns Point Rd, Cambridge, MD 21613 USA.
E-mail Address bcrump@hpl.umces.edu
ORCID Number Crump, Byron/0000-0001-7062-1273
Funding Agency and Grant Number National Oceanic and Atmospheric Administration Chesapeake Bay Office [NA04NMF4570415]
Funding Text This research was supported by grant NA04NMF4570415 from the National Oceanic and Atmospheric Administration Chesapeake Bay Office.
Cited Reference Count 78
Times Cited 48
Total Times Cited Count (WoS, BCI, and CSCD) 50
Publisher AMER SOC MICROBIOLOGY
Publisher City WASHINGTON
Publisher Address 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
ISSN 0099-2240
29-Character Source Abbreviation APPL ENVIRON MICROB
ISO Source Abbreviation Appl. Environ. Microbiol.
Publication Date OCT
Year Published 2008
Volume 74
Issue 19
Beginning Page 5948
Ending Page 5957
Digital Object Identifier (DOI) 10.1128/AEM.00952-08
Page Count 10
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number 352WS
Unique Article Identifier WOS:000259528700012
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