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Publication Type J
Authors Weidner, S; Arnold, W; Stackebrandt, E; Puhler, A
Author Full Name Weidner, S; Arnold, W; Stackebrandt, E; Puhler, A
Title Phylogenetic analysis of bacterial communities associated with leaves of the seagrass Halophila stipulacea by a culture-independent small-subunit rRNA gene approach
Source MICROBIAL ECOLOGY
Language English
Document Type Article
Keywords Plus 16S RIBOSOMAL-RNA; NITROGEN-FIXATION; SP-NOV; MARINE BACTERIOPLANKTON; DIVERSITY; ENVIRONMENT; IDENTIFICATION; ALIGNMENT; SEDIMENTS; SEAWATER
Abstract The phylogenetic diversity of the bacterial community associated with leaves of the marine plant Halophila stipulacea in the northern Gulf of Elat was examined by 16S rRNA gene (rDNA) sequence analyses of a clone library. For 59 clones corresponding to 51 ARDRA (amplified rDNA restriction analysis) groups, the sequence of similar to 1 kb was determined, and the fraction of the corresponding ARDRA groups of the leaf library was calculated. The class Proteobacteria was represented by 62.6% of the clone sequences. Most sequences originated from members of the gamma-subclass (27.3%), affiliated with members of the genera Pseudomonas, Vibrio, Marinomonas, Oceanospirillum, and other marine groups. Affiliation to the alpha-subclass was determined for 24.2% of the sequences. They were related to the genera Hyphomonas, Roseobacter, Ruegeria, and Rhizobiaceae. Several alpha-proteobacterial sequences were distantly related to known sequences. Only 4% of the clone sequences were related to beta-Proteobacteria. Additionally, 7.1% of the sequences possibly belonged to the class Proteobacteria, but branched deeply from known subclasses. Several sequences were affiliated to members of the orders Verrucomicrobiales and Planctomycetales, the Holophaga/Acidobacterium phylum, and chloroplasts of marine diatoms.
Author Address Univ Bielefeld, Fak Biol, Lehrstuhl Genet, D-33615 Bielefeld, Germany; Deutsch Sammlung Mikroorganismen & Zellkulturen G, D-38124 Braunschweig, Germany
Reprint Address Puhler, A (reprint author), Univ Bielefeld, Fak Biol, Lehrstuhl Genet, Univ Str 25, D-33615 Bielefeld, Germany.
ORCID Number Stackebrandt, Erko/0000-0002-6130-760X
Cited Reference Count 49
Times Cited 46
Total Times Cited Count (WoS, BCI, and CSCD) 48
Publisher SPRINGER VERLAG
Publisher City NEW YORK
Publisher Address 175 FIFTH AVE, NEW YORK, NY 10010 USA
ISSN 0095-3628
29-Character Source Abbreviation MICROBIAL ECOL
ISO Source Abbreviation Microb. Ecol.
Publication Date JAN
Year Published 2000
Volume 39
Issue 1
Beginning Page 22
Ending Page 31
Digital Object Identifier (DOI) 10.1007/s002489900194
Page Count 10
Web of Science Category Ecology; Marine & Freshwater Biology; Microbiology
Subject Category Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology
Document Delivery Number 307WJ
Unique Article Identifier WOS:000086676900003
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