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Publication Type J
Authors Gargiulo, GM; Vilardo, I; Cambrea, G; Gemelli, F; Crosca, A
Author Full Name Gargiulo, G. M.; Vilardo, I.; Cambrea, G.; Gemelli, F.; Crosca, A.
Title Karyomorphology and DNA quantification in the marine angiosperm Halophila stipulacea (Forsskal) Ascherson from Mediterranean and Red Seas
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords Seagrasses; Halophila; Chromosomes; Genome size; Karyotype constancy
Keywords Plus GENOME SIZE VARIATION; CHROMOSOME DIVERSITY; EVOLUTION; HYDROCHARITACEAE; KARYOTYPE; SEAGRASS; AMARYLLIDACEAE; CYTOTAXONOMY; CYTOMETRY
Abstract Cytogenetic studies for systematic and evolutionary purposes are lacking on marine phanerogams. Halophila stipulacea is a tropical seagrass that invaded the Mediterranean from the Red Sea through the Suez Canal. In this study, we report, for the first time, the karyotype features and genome size of H. stipulacea populations from different localities. Also, an attempt to compare our data with those reported in literature on other Halophila species is discussed. To determine the karyological relationships among taxa, we used basic karyotypic features such as chromosome number, size and symmetry, and position of chromosomal landmarks. To estimate karyotype asymmetry, the Coefficient of Variation of Chromosome Length (CVCL) and the Mean Centromeric Asymmetry (M-CA) values were calculated. The estimations of DNA amounts were carried out by Image Cytometry (IC) using the interphase-peak method. All the taxa, including those reported in literature, present a uniform and slightly bimodal karyotype, but significant differences are found in nuclear DNA content among species. Each taxa had a karyotype formula 2n = 18 = 10m + 2sm + 6st. Aneuploidy and polyploidy seemed to not be involved in the speciation of this genus. The conserved karyotype in these taxa suggests the presence of mechanisms selecting for constancy, or against changes, in karyotype number and morphology.
Author Address [Gargiulo, G. M.; Vilardo, I.; Cambrea, G.; Crosca, A.] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Via F Stagno DAlcontres 31, I-98166 Messina, Italy; [Gemelli, F.] Univ Tasmania, IMAS, Hobart, Tas 7004, Australia
Reprint Address Gargiulo, GM (reprint author), Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Via F Stagno DAlcontres 31, I-98166 Messina, Italy.
E-mail Address ggargiulo@unime.it
Cited Reference Count 64
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date AUG
Year Published 2018
Volume 148
Beginning Page 1
Ending Page 9
Digital Object Identifier (DOI) 10.1016/j.aquabot.2018.04.001
Page Count 9
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number GH7SB
Unique Article Identifier WOS:000433652600001
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