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| Publication Type | J |
| Authors | Yuvaraj, N; Kanmani, P; Satishkumar, R; Paari, A; Pattukumar, V; Arul, V |
| Author Full Name | Yuvaraj, N.; Kanmani, P.; Satishkumar, R.; Paari, A.; Pattukumar, V.; Arul, V. |
| Title | Seagrass as a potential source of natural antioxidant and anti-inflammatory agents |
| Source | PHARMACEUTICAL BIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | Antibacterial; GC-MS; Halophila ovalis; MIC; triacylglycerols |
| Keywords Plus | ANTIMICROBIAL ACTIVITIES; PURE COMPOUND; MAP KINASES; TNF-ALPHA; EXTRACTS; INHIBITION; ALGAE; COAST; ANTIBACTERIAL; MACROALGAE |
| Abstract | Context: Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements. Objective: The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed. Materials and methods: Plant materials were collected from Pondicherry coastal line, and antimicrobial screening of crude extract, and purified fractions was carried out by the disc diffusion method and the minimum inhibitory concentration (MICs) of the purified fractions and reference antibiotics were determined by microdilution method. Antioxidant and anti-inflammatory activities were investigated in vitro. Chemical constituents of purified fractions V and VI were analyzed by gas chromatography-mass spectrometry (GC-MS), and the phytochemicals were quantitatively determined. Results: Methanol extract inhibited the growth of Bacillus cereus at a minimum inhibitory concentration of 50 mu g/mL and other Gram-negative pathogens at 75 mu g/mL, except Vibrio vulnificus. Reducing power and total antioxidant level increased with increasing extract concentration. H. ovalis exhibited strong scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals at IC50 of 0.13 and 0.65 mg/mL, respectively. Methanol extract of H. ovalis showed noticeable anti-inflammatory activity at IC50 of 78.72 mu g/mL. The GC-MS analysis of H. ovalis revealed the presence of triacylglycerols as major components in purified fractions. Quantitative analysis of phytochemicals revealed that phenols are rich in seagrass H. ovalis. Discussion and conclusion: These findings demonstrated that the methanol extract of H. ovalis exhibited appreciable antibacterial, noticeable antioxidant, and anti-inflammatory activities, and thus could be use as a potential source for natural health products. |
| Author Address | [Yuvaraj, N.; Kanmani, P.; Satishkumar, R.; Paari, A.; Pattukumar, V.; Arul, V.] Pondicherry Univ, Dept Biotechnol, Pondicherry 605014, India |
| Reprint Address | Arul, V (reprint author), Pondicherry Univ, Dept Biotechnol, Pondicherry 605014, India. |
| E-mail Address | varul18@yahoo.com |
| Funding Agency and Grant Number | Department of Biotechnology (DBT), New Delhi, India |
| Funding Text | This work was supported by a grant from Department of Biotechnology (DBT), New Delhi, India. The authors declare that there are no other conflicts of interest. |
| Cited Reference Count | 50 |
| Times Cited | 8 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 8 |
| Publisher | TAYLOR & FRANCIS LTD |
| Publisher City | ABINGDON |
| Publisher Address | 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND |
| ISSN | 1388-0209 |
| 29-Character Source Abbreviation | PHARM BIOL |
| ISO Source Abbreviation | Pharm. Biol. |
| Publication Date | APR |
| Year Published | 2012 |
| Volume | 50 |
| Issue | 4 |
| Beginning Page | 458 |
| Ending Page | 467 |
| Digital Object Identifier (DOI) | 10.3109/13880209.2011.611948 |
| Page Count | 10 |
| Web of Science Category | Plant Sciences; Medical Laboratory Technology; Pharmacology & Pharmacy |
| Subject Category | Plant Sciences; Medical Laboratory Technology; Pharmacology & Pharmacy |
| Document Delivery Number | 905VL |
| Unique Article Identifier | WOS:000301304100009 |
| Plants associated with this reference |