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Publication Type J
Authors Yuvaraj, N; Kanmani, P; Satishkumar, R; Paari, A; Pattukumar, V; Arul, V
Author Full Name Yuvaraj, N.; Kanmani, P.; Satishkumar, R.; Paari, A.; Pattukumar, V.; Arul, V.
Title Seagrass as a potential source of natural antioxidant and anti-inflammatory agents
Source PHARMACEUTICAL BIOLOGY
Language English
Document Type Article
Author Keywords Antibacterial; GC-MS; Halophila ovalis; MIC; triacylglycerols
Keywords Plus ANTIMICROBIAL ACTIVITIES; PURE COMPOUND; MAP KINASES; TNF-ALPHA; EXTRACTS; INHIBITION; ALGAE; COAST; ANTIBACTERIAL; MACROALGAE
Abstract Context: Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements. Objective: The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed. Materials and methods: Plant materials were collected from Pondicherry coastal line, and antimicrobial screening of crude extract, and purified fractions was carried out by the disc diffusion method and the minimum inhibitory concentration (MICs) of the purified fractions and reference antibiotics were determined by microdilution method. Antioxidant and anti-inflammatory activities were investigated in vitro. Chemical constituents of purified fractions V and VI were analyzed by gas chromatography-mass spectrometry (GC-MS), and the phytochemicals were quantitatively determined. Results: Methanol extract inhibited the growth of Bacillus cereus at a minimum inhibitory concentration of 50 mu g/mL and other Gram-negative pathogens at 75 mu g/mL, except Vibrio vulnificus. Reducing power and total antioxidant level increased with increasing extract concentration. H. ovalis exhibited strong scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals at IC50 of 0.13 and 0.65 mg/mL, respectively. Methanol extract of H. ovalis showed noticeable anti-inflammatory activity at IC50 of 78.72 mu g/mL. The GC-MS analysis of H. ovalis revealed the presence of triacylglycerols as major components in purified fractions. Quantitative analysis of phytochemicals revealed that phenols are rich in seagrass H. ovalis. Discussion and conclusion: These findings demonstrated that the methanol extract of H. ovalis exhibited appreciable antibacterial, noticeable antioxidant, and anti-inflammatory activities, and thus could be use as a potential source for natural health products.
Author Address [Yuvaraj, N.; Kanmani, P.; Satishkumar, R.; Paari, A.; Pattukumar, V.; Arul, V.] Pondicherry Univ, Dept Biotechnol, Pondicherry 605014, India
Reprint Address Arul, V (reprint author), Pondicherry Univ, Dept Biotechnol, Pondicherry 605014, India.
E-mail Address varul18@yahoo.com
Funding Agency and Grant Number Department of Biotechnology (DBT), New Delhi, India
Funding Text This work was supported by a grant from Department of Biotechnology (DBT), New Delhi, India. The authors declare that there are no other conflicts of interest.
Cited Reference Count 50
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher TAYLOR & FRANCIS LTD
Publisher City ABINGDON
Publisher Address 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
ISSN 1388-0209
29-Character Source Abbreviation PHARM BIOL
ISO Source Abbreviation Pharm. Biol.
Publication Date APR
Year Published 2012
Volume 50
Issue 4
Beginning Page 458
Ending Page 467
Digital Object Identifier (DOI) 10.3109/13880209.2011.611948
Page Count 10
Web of Science Category Plant Sciences; Medical Laboratory Technology; Pharmacology & Pharmacy
Subject Category Plant Sciences; Medical Laboratory Technology; Pharmacology & Pharmacy
Document Delivery Number 905VL
Unique Article Identifier WOS:000301304100009
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