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Publication Type J
Authors Supaphon, P; Phongpaichit, S; Rukachaisirikul, V; Sakayaroj, J
Author Full Name Supaphon, Preuttiporn; Phongpaichit, Souwalak; Rukachaisirikul, Vatcharin; Sakayaroj, Jariya
Title Antimicrobial Potential of Endophytic Fungi Derived from Three Seagrass Species: Cymodocea serrulata, Halophila ovalis and Thalassia hemprichii
Source PLOS ONE
Language English
Document Type Article
Keywords Plus NATURAL-PRODUCTS; SECONDARY METABOLITES; MEDICINAL-PLANTS; ANTIBACTERIAL; PHYTOCHEMICALS; TRICHODERMA; DERIVATIVES; CHINA
Abstract Endophytic fungi from three commonly found seagrasses in southern Thailand were explored for their ability to produce antimicrobial metabolites. One hundred and sixty endophytic fungi derived from Cymodocea serrulata (Family Cymodoceaceae), Halophila ovalis and Thalassia hemprichii (Family Hydrocharitaceae) were screened for production of antimicrobial compounds by a colorimetric broth microdilution test against ten human pathogenic microorganisms including Staphylococcus aureus ATCC 25923, a clinical isolate of methicillin-resistant S. aureus, Escherichia coli ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 90028 and NCPF 3153, Cryptococcus neoformans ATCC 90112 and ATCC 90113 and clinical isolates of Microsporum gypseum and Penicillium marneffei. Sixty-nine percent of the isolates exhibited antimicrobial activity against at least one test strain. Antifungal activity was more pronounced than antibacterial activity. Among the active fungi, seven isolates including Hypocreales sp. PSU-ES26 from C. serrulata, Trichoderma spp. PSU-ES8 and PSU-ES38 from H. ovalis, and Penicillium sp. PSU-ES43, Fusarium sp. PSU-ES73, Stephanonectria sp. PSU-ES172 and an unidentified endophyte PSU-ES190 from T. hemprichii exhibited strong antimicrobial activity against human pathogens with minimum inhibitory concentrations (MIC) of less than 10 mu g/ml. The inhibitory extracts at concentrations of 4 times their MIC destroyed the targeted cells as observed by scanning electron microscopy. These results showed the antimicrobial potential of extracts from endophytic fungi from seagrasses.
Author Address [Supaphon, Preuttiporn; Phongpaichit, Souwalak] Prince Songkla Univ, Dept Microbiol, Fac Sci, Hat Yai, Songkhla, Thailand; [Supaphon, Preuttiporn; Phongpaichit, Souwalak] Prince Songkla Univ, Nat Prod Res Ctr Excellence, Fac Sci, Hat Yai, Songkhla, Thailand; [Rukachaisirikul, Vatcharin] Prince Songkla Univ, Dept Chem, Fac Sci, Hat Yai, Songkhla, Thailand; [Rukachaisirikul, Vatcharin] Prince Songkla Univ, Ctr Excellence Innovat Chem, Fac Sci, Hat Yai, Songkhla, Thailand; [Sakayaroj, Jariya] Natl Ctr Genet Engn & Biotechnol, Klongluang, Pathumthani, Thailand
Reprint Address Phongpaichit, S (reprint author), Prince Songkla Univ, Dept Microbiol, Fac Sci, Hat Yai, Songkhla, Thailand.
E-mail Address souwalak.p@psu.ac.th
Funding Agency and Grant Number Thailand Research Fund (TRF) [RTA5480002, PHD/0052/2550]; Prince of Songkla University
Funding Text This work was supported by the Thailand Research Fund (TRF) Senior Research Scholar (Grant no. RTA5480002, the Center of Excellence for Innovationin Chemistry (PERCH-CIC) and Graduate School, Prince of Songkla University. PS is grateful to the TRF through the Royal Golden Jubilee Ph.D. Program (Grant no. PHD/0052/2550) and Prince of Songkla University for scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Cited Reference Count 44
Times Cited 21
Total Times Cited Count (WoS, BCI, and CSCD) 23
Publisher PUBLIC LIBRARY SCIENCE
Publisher City SAN FRANCISCO
Publisher Address 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
ISSN 1932-6203
29-Character Source Abbreviation PLOS ONE
ISO Source Abbreviation PLoS One
Publication Date AUG 16
Year Published 2013
Volume 8
Issue 8
Article Number e72520
Digital Object Identifier (DOI) 10.1371/journal.pone.0072520
Page Count 9
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number 207BA
Unique Article Identifier WOS:000323570200072
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