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Publication Type J
Authors Nguyen, VX; Detcharoen, M; Tuntiprapas, P; Soe-Htun, U; Sidik, JB; Harah, MZ; Prathep, A; Papenbrock, J
Author Full Name Nguyen, Vy X.; Detcharoen, Matsapume; Tuntiprapas, Piyalap; Soe-Htun, U.; Sidik, Japar B.; Harah, Muta Z.; Prathep, Anchana; Papenbrock, Jutta
Title Genetic species identification and population structure of Halophila (Hydrocharitaceae) from the Western Pacific to the Eastern Indian Ocean
Source BMC EVOLUTIONARY BIOLOGY
Language English
Document Type Article
Author Keywords Eastern Indian Ocean; Evolution; Genetic distance; Halophila ovalis; Western Pacific Ocean
Keywords Plus POSIDONIA-OCEANICA; MAXIMUM-LIKELIHOOD; PHYLOGENETIC TREES; CYMODOCEA-NODOSA; ZOSTERA-MARINA; SEQUENCE DATA; AFLP MARKERS; DIVERSITY; SEAGRASS; DNA
Abstract Background: The Indo-Pacific region has the largest number of seagrass species worldwide and this region is considered as the origin of the Hydrocharitaceae. Halophila ovalis and its closely-related species belonging to the Hydrocharitaceae are well-known as a complex taxonomic challenge mainly due to their high morphological plasticity. The relationship of genetic differentiation and geographic barriers of H. ovalis radiation was not much studied in this region. Are there misidentifications between H. ovalis and its closely related species? Does any taxonomic uncertainty among different populations of H. ovalis persist? Is there any genetic differentiation among populations in the Western Pacific and the Eastern Indian Ocean, which are separated by the Thai-Malay peninsula? Genetic markers can be used to characterize and identify individuals or species and will be used to answer these questions. Results: Phylogenetic analyses of the nuclear ribosomal internal transcribed spacer region based on materials collected from 17 populations in the Western Pacific and the Eastern Indian Ocean showed that some specimens identified as H. ovalis belonged to the H. major clade, also supported by morphological data. Evolutionary divergence between the two clades is between 0.033 and 0.038, much higher than the evolutionary divergence among H. ovalis populations. Eight haplotypes were found; none of the haplotypes from the Western Pacific is found in India and vice versa. Analysis of genetic diversity based on microsatellite analysis revealed that the genetic diversity in the Western Pacific is higher than in the Eastern Indian Ocean. The unrooted neighbor-joining tree among 14 populations from the Western Pacific and the Eastern Indian Ocean showed six groups. The Mantel test results revealed a significant correlation between genetic and geographic distances among populations. Results from band-based and allele frequency-based approaches from Amplified Fragment Length Polymorphism showed that all samples collected from both sides of the Thai-Malay peninsula were clustered into two clades: Gulf of Thailand and Andaman Sea. Conclusions: Our study documented the new records of H. major for Malaysia and Myanmar. The study also revealed that the Thai-Malay peninsula is a geographic barrier between H. ovalis populations in the Western Pacific and the Eastern Indian Ocean.
Author Address [Nguyen, Vy X.; Papenbrock, Jutta] Leibniz Univ Hannover, Inst Bot, D-30419 Hannover, Germany; [Nguyen, Vy X.] Vietnam Acad Sci & Technol, Inst Oceanog, Dept Marine Botan, Nha Trang City, Vietnam; [Detcharoen, Matsapume; Prathep, Anchana] Prince Songkla Univ, Fac Sci, Dept Biol, Hat Yai 90112, Songkhla, Thailand; [Tuntiprapas, Piyalap] Prince Songkla Univ, Fac Sci, Excellence Ctr Biodivers Peninsular Thailand, Seaweed & Seagrass Res Unit, Hat Yai 90112, Songkhla, Thailand; [Soe-Htun, U.] Mawlamyine Univ, Dept Marine Sci, Mawlamyine, Myanmar; [Sidik, Japar B.] Univ Putra Malaysia, Fac Agr & Food Sci, Dept Anim Sci & Fishery, Bintulu 97008, Sarawak, Malaysia; [Harah, Muta Z.] Univ Putra Malaysia, Fac Agr, Dept Aquaculture, Serdang 43400, Selangor Darul, Malaysia
Reprint Address Papenbrock, J (reprint author), Leibniz Univ Hannover, Inst Bot, Herrenhauserstr 2, D-30419 Hannover, Germany.
E-mail Address Jutta.Papenbrock@botanik.uni-hannover.de
ORCID Number bujang, japar sidik/0000-0002-9797-7963
Funding Agency and Grant Number Leibniz University Hannover, Hannover, Germany; Ministry of Education and Training, Viet Nam; Prince of Songkla University; Higher Education Research Promotion; National Research University Project of Thailand; Office of The Higher Education Commission, Universiti Putra Malaysia; Ministry of Science, Technology and Innovation (MOSTI), Malaysia [04-01-04-SF1171]; Deutsche Forschungsgemeinschaft; Leibniz Universitat Hannover
Funding Text This work was financially supported by the Leibniz University Hannover, Hannover, Germany; the Ministry of Education and Training, Viet Nam; the Prince of Songkla University, the Higher Education Research Promotion and National Research University Project of Thailand; the Office of The Higher Education Commission, Universiti Putra Malaysia and Science Fund 04-01-04-SF1171, Ministry of Science, Technology and Innovation (MOSTI), Malaysia. We are deeply indebted to Prof. Dr. T. Debener and Dr. M. Linde for giving technical and scientific advice on AFLP and SSRs analysis and for the possibility to use their equipment. We would like to thank Mr. Felix Hirschmann, Leibniz University Hannover, Germany, for correcting the English language and for collecting samples together with the esteemed colleagues at the Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, India. Thanks to Prof. Put O Ang, Jr for sample collection in Hong Kong. We acknowledge support by Deutsche Forschungsgemeinschaft and Open Access Publishing Fund of Leibniz Universitat Hannover.
Cited Reference Count 87
Times Cited 18
Total Times Cited Count (WoS, BCI, and CSCD) 18
Publisher BIOMED CENTRAL LTD
Publisher City LONDON
Publisher Address 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
ISSN 1471-2148
29-Character Source Abbreviation BMC EVOL BIOL
ISO Source Abbreviation BMC Evol. Biol.
Publication Date APR 30
Year Published 2014
Volume 14
Article Number 92
Digital Object Identifier (DOI) 10.1186/1471-2148-14-92
Page Count 18
Web of Science Category Evolutionary Biology; Genetics & Heredity
Subject Category Evolutionary Biology; Genetics & Heredity
Document Delivery Number AH3WQ
Unique Article Identifier WOS:000336057300001
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