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Publication Type J
Authors Nguyen, XV; Hofler, S; Glasenapp, Y; Thangaradjou, T; Lucas, C; Papenbrock, J
Author Full Name Xuan-Vy Nguyen; Hoefler, Saskia; Glasenapp, Yvana; Thangaradjou, Thirunavukarassu; Lucas, Christina; Papenbrock, Jutta
Title New insights into DNA barcoding of seagrasses
Source SYSTEMATICS AND BIODIVERSITY
Language English
Document Type Article
Author Keywords genetic diversity; Halophila; ITS; matK; rbcL; seagrass
Keywords Plus ROOTED PHYLOGENETIC TREES; GENUS HALOPHILA THOUARS; MAXIMUM-LIKELIHOOD; SEQUENCE DATA; HYDROCHARITACEAE; ZOSTERACEAE; DIVERSIFICATION; CYMODOCEACEAE; ALISMATIDAE; ANGIOSPERMS
Abstract Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as DNA barcodes'. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.
Author Address [Xuan-Vy Nguyen] Vietnam Acad Sci & Technol, Inst Oceanog, Nha Trang City, Vietnam; [Hoefler, Saskia; Glasenapp, Yvana; Papenbrock, Jutta] Leibniz Univ Hannover, Inst Bot, D-30167 Hannover, Germany; [Thangaradjou, Thirunavukarassu] Annamalai Univ, Fac Marine Sci, Ctr Adv Study Marine Biol, Parangipettai, India; [Lucas, Christina] Univ Cologne, Bioctr, D-50931 Cologne, Germany
Reprint Address Papenbrock, J (reprint author), Leibniz Univ Hannover, Inst Bot, D-30167 Hannover, Germany.
E-mail Address jutta.papenbrock@botanik.uni-hannover.de
Cited Reference Count 54
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 7
Publisher TAYLOR & FRANCIS LTD
Publisher City ABINGDON
Publisher Address 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
ISSN 1477-2000
29-Character Source Abbreviation SYST BIODIVERS
ISO Source Abbreviation Syst. Biodivers.
Publication Date SEP 3
Year Published 2015
Volume 13
Issue 5
Beginning Page 496
Ending Page 508
Digital Object Identifier (DOI) 10.1080/14772000.2015.1046408
Page Count 13
Web of Science Category Biodiversity Conservation; Biology
Subject Category Biodiversity & Conservation; Life Sciences & Biomedicine - Other Topics
Document Delivery Number CP4QK
Unique Article Identifier WOS:000359867200007
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