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Publication Type J
Authors Trotta, A., S. Barera, F. Marsano, D. Osella, D. Musso, C. Pagliano, F. Andreucci and R. Barbato
Title Isolation and characterization of a photosystem II preparation from thylakoid membranes of the extreme halophyte Salicomia veneta Pignatti et Lausi
Source Plant Physiology and Biochemistry
Language English
Author Keywords Photosystem II Halophyte Salicornia veneta oxygen-evolving complex synechocystis sp pcc-6803 higher-plants psbp protein photoinhibition arabidopsis assembly/stability photoautotrophy polypeptide spinach Plant Sciences
Abstract Salicomia veneta (Pignatti et Lausi) is an extreme halophyte living in salt marsh where NaCl concentration may be as high as 1 M. Here we report on the isolation and characterization of a PSII preparation obtained by Triton X-100 solubilisation of the thylakoid membrane. By a combination of gel electrophoresis, immunoblotting and mass spectrometry, the depletion of a number of PSII proteins such as PsbQ, PsbM and PsbT was highlighted. Moreover, the requirement of Cl(- )and Ca2+ for optimal oxygen evolution was determined, showing that in absence of PsbQ a higher level of these ions are required. At high Cl(- )concentrations, oxygen evolution was inhibited in the same way in Salicomia veneta and spinach. Reconstitution of Salicomia veneta PSII preparation with partially purified spinach PsbP and PsbQ restored oxygen evolution activity at low Cl- and Ca2+ concentrations. Adaptation to high salt makes several PSII proteins dispensable.
Author Address [Trotta, Andrea; Barera, Simone; Marsano, Francesco; Osella, Domenico; Musso, Davide; Andreucci, Flora; Barbato, Roberto] Univ Piemonte Orientale, Dipartimento Sci & Innovaz Tecnol, Viale Michel 11, I-15121 Alessandria, Italy. [Trotta, Andrea] Univ Turku, Dept Biochem, Mol Plant Biol, FI-20014 Turku, Finland. [Pagliano, Cristina] Politecn Torino, Dipartimento Sci Applicata & Tecnol, Viale Duca Abruzzi 24, I-10129 Turin, Italy. Barbato, R (reprint author), Univ Piemonte Orientale, Dipartimento Sci & Innovaz Tecnol, Viale Michel 11, I-15121 Alessandria, Italy. roberto.barbato@uniupo.it
ISSN 0981-9428
ISBN 0981-9428
29-Character Source Abbreviation Plant Physiol. Biochem.
Publication Date Nov
Year Published 2018
Volume 132
Beginning Page 356-362
Digital Object Identifier (DOI) 10.1016/j.plaphy.2018.09.023
Unique Article Identifier WOS:000450377400037
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