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Publication Type J
Authors Ben Saad, R., A. Ben Hsouna, W. Saibi, K. Ben Hamed, F. Brini and T. Ghneim-Herrera
Title A stress-associated protein, LmSAP, from the halophyte Lobularia maritima provides tolerance to heavy metals in tobacco through increased ROS scavenging and metal detoxification processes
Source Journal of Plant Physiology
Language English
Author Keywords Lobularia maritima LmSAP gene Heavy metal tolerance Oxidative stress Metal accumulation regulates multiple genes induced oxidative stress a20/an1 zinc-finger transcription factor aluminum tolerance cadmium tolerance metallothionein gene confers tolerance arabidopsis-thaliana transgenic tobacco Plant Sciences
Abstract Agricultural soil pollution by heavy metals is a severe global ecological problem. We recently showed that overexpression of LmSAP, a member of the stress-associated protein (SAP) gene family isolated from Lobularia maritima, in transgenic tobacco led to enhanced tolerance to abiotic stress. In this study, we characterised the response of LmSAP transgenic tobacco plants to metal stresses (cadmium (Cd), copper (Cu), manganese (Mn), and zinc (Zn)). In L. maritima, LmSAP expression increased after 12 h of treatment with these metals, suggesting its involvement in the plant response to heavy metal stress. LmSAP transgenic tobacco plants subjected to these stress conditions were healthy, experienced higher seedling survival rates, and had longer roots than non-transgenic plants (NT). However, they exhibited higher tolerance towards cadmium and manganese than towards copper and zinc. LmSAP-overexpressing tobacco seedlings accumulated more cadmium, copper, and manganese compared with NT plants, but displayed markedly decreased hydrogen peroxide (H2O2) and lipid peroxidation levels after metal treatment. Activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were significantly higher in transgenic plants than in NT plants after exposure to metal stress. LmSAP overexpression also enhanced the transcription of several genes encoding metallothioneins (Metl, Met2, Met3, Met4, and Met5), a copper transport protein CCH, a Cys and His-rich domain-containing protein RAR1 (Rar1), and a ubiquitin-like protein 5 (PUB1), which are involved in metal tolerance in tobacco. Our findings indicate that LmSAP overexpression in tobacco enhanced tolerance to heavy metal stress by protecting the plant cells against oxidative stress, scavenging reactive oxygen species (ROS), and decreasing the intracellular concentration of free heavy metals through its effect on metal-binding proteins in the cytosol.
Author Address [Ben Saad, Rania; Ben Hsouna, Anis; Saibi, Walid; Brini, Faical] Univ Sfax, Ctr Biotechnol Sfax, Biotechnol & Plant Improvement Lab, BP 1177, Sfax 3018, Tunisia. [Ben Hsouna, Anis] Fac Sci Gafsa, Dept Life Sci, Zarroug 2112, Gafsa, Tunisia. [Ben Hamed, Karim] Ctr Biotechnol Borj Cedria, Lab Plantes Extremophiles, BP 901, Hammam Lif 2050, Tunisia. [Ghneim-Herrera, Thaura] Univ Icesi, Dept Ciencias Biol, Cali, Colombia. Ghneim-Herrera, T (reprint author), Univ Icesi, Dept Ciencias Biol, Cali, Colombia. tghneim@icesi.edu.co
ISSN 0176-1617
ISBN 0176-1617
29-Character Source Abbreviation J. Plant Physiol.
Publication Date Dec
Year Published 2018
Volume 231
Beginning Page 234-243
Digital Object Identifier (DOI) 10.1016/j.jplph.2018.09.019
Unique Article Identifier WOS:000451619200023
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