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| Publication Type | J |
| Authors | Torzilli, AP; Sikaroodi, M; Chalkley, D; Gillevet, PM |
| Author Full Name | Torzilli, Albert P.; Sikaroodi, Masoumeh; Chalkley, David; Gillevet, Patrick M. |
| Title | A comparison of fungal communities from four salt marsh plants using automated ribosomal intergenic spacer analysis (ARISA) |
| Source | MYCOLOGIA |
| Language | English |
| Document Type | Article |
| Author Keywords | automated ribosomal intergenic spacer analysis; Distichlis; fungal community fingerprinting; Juncus; salt marsh fungi; Sarcocornia; Spartina |
| Keywords Plus | SPARTINA-ALTERNIFLORA; RHODE-ISLAND; COPY NUMBER; RNA GENES; BIAS; PCR; DYNAMICS; HETEROGENEITY; AMPLIFICATION; DEGRADATION |
| Abstract | Fungal decomposers are important contributors to the detritus-based food webs of salt marsh ecosystems. Knowing the composition of salt marsh fungal communities is essential in understanding how detritus processing is affected by changes in community dynamics. Automated ribosomal intergenic spacer analysis (ARISA) was used to examine the composition of fungal communities associated with four temperate salt marsh plants, Spartina alterniflora (short and tall forms), Juncus roemerianus, Distichlis spicala and Sarcocornia per-ennis. Plant tissues were homogenized and subjected to a particle-filtration protocol that yielded 106 mu m particulate fractions, which were used as a Source of fungal isolates and fungal DNA. Genera identified from sporulating cultures demonstrated that the 106 mu m particles from each host plant were reliable sources of fungal DNA for ARISA. Analysis of ARISA data by principal component analysis (PCA), principal coordinate analysis (PCO) and species diversity comparisons indicated that the fungal communities from the two grasses, S. alterniflora and D. spicala were more similar to each other than they were to the distinct communities associated with J. roemerianus and S. perennis. Principal component analysis also showed no consistent, seasonal pattern in the composition of these fungal communities. Comparisons of ARISA fingerprints from the different fungal communities and those from pure Cultures of selected Spartina ascomycetes supported the host/substrate specificity observed for the fungal communities. |
| Author Address | George Mason Univ, Dept Environm Sci & Policy, Fairfax, VA 22030 USA; George Mason Univ, Dept Environm Sci & Policy, Manassas, VA 20110 USA; Amer Type Culture Collect, Manassas, VA 20110 USA |
| Reprint Address | Torzilli, AP (reprint author), George Mason Univ, Dept Environm Sci & Policy, Fairfax, VA 22030 USA. |
| E-mail Address | atorzill@gmu.edu |
| Cited Reference Count | 28 |
| Times Cited | 29 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 32 |
| Publisher | ALLEN PRESS INC |
| Publisher City | LAWRENCE |
| Publisher Address | 810 E 10TH ST, LAWRENCE, KS 66044 USA |
| ISSN | 0027-5514 |
| 29-Character Source Abbreviation | MYCOLOGIA |
| ISO Source Abbreviation | Mycologia |
| Publication Date | SEP-OCT |
| Year Published | 2006 |
| Volume | 98 |
| Issue | 5 |
| Beginning Page | 690 |
| Ending Page | 698 |
| Digital Object Identifier (DOI) | 10.3852/mycologia.98.5.690 |
| Page Count | 9 |
| Web of Science Category | Mycology |
| Subject Category | Mycology |
| Document Delivery Number | 125JP |
| Unique Article Identifier | WOS:000243438500004 |
| Plants associated with this reference |