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Publication Type J
Authors Wang, J. C., B. C. Li, L. R. Yao, Y. X. Meng, X. L. Ma, Y. Lai, E. J. Si, P. R. Ren, K. Yang, X. W. Shang and H. J. Wang
Title Comparative transcriptome analysis of genes involved in Na+ transport in the leaves of halophyte Halogeton glomeratus
Source Gene
Language English
Author Keywords Halophyte H. glomeratus Salt stress Na+ compartmentalization Transcriptome salt-stress-response salinity tolerance arabidopsis-thaliana antiporters homeostasis mechanisms growth plants crops Genetics & Heredity
Abstract Compartmentalization of Na+ into vacuoles is considered to be the most critical aspect of salt tolerance in H. glomeratus, an annual, succulent halophyte. Previous analysis of transcriptome involved in the H. glomeratus salt stress response relied on next-generation sequencing technologies that limit the capture of accurately spliced, full-length isoforms. To gain deeper insights into its salt stress response, we used the H. glomeratus Iso-Seq transcriptome database as a reference, and subsequent next-generation sequencing was subjected to various NaCl concentrations of leaves from plants revealed 115 upregulated and 87 downregulated differentially expressed isoforms (core DEIs). The majority of the core DEIs were involved in carbohydrate metabolism and energy production and conversion. In contrast, levels of known isoforms encoding Na+ transporters did not change significantly under salt stress. However, 16 core DEIs of unknown function were predicted to possess transmembrane domains, suggesting that these candidate isoforms could be involved in Na+ transport in H. glomeratus. These results suggest a potential means for identification of novel Na+ transporters, in addition to providing a foundation for further investigation of Na+ transport networks in halophytes.
Author Address [Wang, Juncheng; Li, Baochun; Yao, Lirong; Meng, Yaxiong; Ma, Xiaole; Si, Erjing; Ren, Panrong; Yang, Ke; Wang, Huajun] Gansu Key Lab Crop Improvement & Germplasm Enhanc, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou, Gansu, Peoples R China. [Li, Baochun] Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou, Gansu, Peoples R China. [Wang, Juncheng; Yao, Lirong; Meng, Yaxiong; Ma, Xiaole; Si, Erjing; Ren, Panrong; Yang, Ke; Shang, Xunwu; Wang, Huajun] Gansu Agr Univ, Coll Agron, Lanzhou, Gansu, Peoples R China. [Lai, Yong] Qinghai Univ, Coll Agr & Anim Husb, Xining, Qinghai, Peoples R China. Wang, HJ (reprint author), Gansu Agr Univ, Coll Agron, Lanzhou, Gansu, Peoples R China. huajunwang@sina.com
ISSN 0378-1119
ISBN 0378-1119
29-Character Source Abbreviation Gene
Publication Date Dec
Year Published 2018
Volume 678
Beginning Page 407-416
Digital Object Identifier (DOI) 10.1016/j.gene.2018.08.025
Unique Article Identifier WOS:000447483000048
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