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Publication Type J
Authors Misic, D; Dragicevic, M; Siler, B; Zivkovic, JN; Maksimovic, V; Momcilovic, I; Nikolic, M
Author Full Name Misic, Danijela; Dragicevic, Milan; Siler, Branislav; Zivkovic, Jasmina Nestorovic; Maksimovic, Vuk; Momcilovic, Ivana; Nikolic, Miroslav
Title Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
Source JOURNAL OF PLANT PHYSIOLOGY
Language English
Document Type Article
Author Keywords Acid invertase; Root culture; Salt stress; Soluble sugars; Sugar uptake
Keywords Plus CELL-WALL INVERTASE; ORGAN-SPECIFIC EXPRESSION; BETA-VULGARIS L.; ARABIDOPSIS-THALIANA; SUSPENSION CULTURES; SUCROSE UTILIZATION; VACUOLAR INVERTASE; PRIMARY METABOLISM; GROWTH; INHIBITOR
Abstract A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
Author Address [Misic, Danijela; Dragicevic, Milan; Siler, Branislav; Zivkovic, Jasmina Nestorovic; Momcilovic, Ivana] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade 11060, Serbia; [Maksimovic, Vuk; Nikolic, Miroslav] Univ Belgrade, Inst Multidisciplinary Res, Belgrade 11000, Serbia
Reprint Address Misic, D (reprint author), Univ Belgrade, Inst Biol Res Sinisa Stankovic, Bulevar Despota Stefano 142, Belgrade 11060, Serbia.
E-mail Address dmisic@ibiss.bg.ac.rs
ResearcherID Number Siler, Branislav/F-8132-2011; Nikolic, Miroslav/H-8657-2014
ORCID Number Siler, Branislav/0000-0001-5831-6612; Nikolic, Miroslav/0000-0001-9643-138X; Misic, Danijela/0000-0002-5419-0773; Maksimovic, Vuk/0000-0002-9883-186X; Momcilovic, Ivana/0000-0002-8365-8224; Dragicevic, Milan/0000-0002-9422-2952
Funding Agency and Grant Number Serbian Ministry of Education and Science [173024, 173028, 173040]
Funding Text We dedicate this work to our dear colleague, Professor Dr. Dragoljub Grubgie. This work is financially supported by the Serbian Ministry of Education and Science (Grants 173024, 173028 and 173040). Authors would like to thank Dr. Alison H. Kingston Smith (Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK) for providing antibodies used for immunodetection of invertase.
Cited Reference Count 44
Times Cited 9
Total Times Cited Count (WoS, BCI, and CSCD) 12
Publisher ELSEVIER GMBH, URBAN & FISCHER VERLAG
Publisher City JENA
Publisher Address OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
ISSN 0176-1617
29-Character Source Abbreviation J PLANT PHYSIOL
ISO Source Abbreviation J. Plant Physiol.
Publication Date SEP 1
Year Published 2012
Volume 169
Issue 13
Beginning Page 1281
Ending Page 1289
Digital Object Identifier (DOI) 10.1016/j.jplph.2012.04.018
Page Count 9
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 012OP
Unique Article Identifier WOS:000309246700007
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