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Publication Type J
Authors WAKE, CM; SCHAEFER, PR; JOST, LK; EVENSON, DP
Author Full Name WAKE, CM; SCHAEFER, PR; JOST, LK; EVENSON, DP
Title ANALYSIS OF INTRASPECIFIC NUCLEAR-DNA CONTENT VARIATION IN GLEDITSIA-TRIACANTHOS BY FLOW-CYTOMETRY
Source CANADIAN JOURNAL OF FOREST RESEARCH
Language English
Document Type Article
Keywords Plus HIGHER-PLANTS; CELL-CYCLE; TISSUES; FLUOROCHROMES
Abstract Honeylocust trees (Gleditsia triacanthos L.) are distributed across a wide range of climatic and edaphic conditions, demonstrating considerable variation in cold, drought, and salinity tolerances. In many angiosperm species, an evolutionary change in nuclear DNA content accompanies colonization into more stressful environments. Fresh leaf nuclei were isolated from 22 honeylocust sources from across the species' native range, mixed with trout red blood cells, stained with propidium iodide, and their DNA content was measured by flow cytometry. According to DNA stainability of the trout red blood cells, leaf nuclei had a DNA content of 1.72 +/- 0.02 pg (mean +/- SD). Variation in DNA content among the sources was not significant (p < 0.05), suggesting that adaptive evolutionary changes in honeylocust are not evident in increasing or decreasing amounts of leaf nuclear DNA content.
Author Address S DAKOTA STATE UNIV, DEPT BIOL MICROBIOL, BROOKINGS, SD 57007 USA; S DAKOTA STATE UNIV, OLSON BIOCHEM LABS, BROOKINGS, SD 57007 USA
Reprint Address WAKE, CM (reprint author), S DAKOTA STATE UNIV, DEPT HORT FORESTRY LANDSCAPE & PK, BOX 2140A, BSL 201, BROOKINGS, SD 57007 USA.
Cited Reference Count 45
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
Publisher City OTTAWA
Publisher Address 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA
ISSN 0045-5067
29-Character Source Abbreviation CAN J FOREST RES
ISO Source Abbreviation Can. J. For. Res.
Publication Date MAR
Year Published 1995
Volume 25
Issue 3
Beginning Page 440
Ending Page 445
Digital Object Identifier (DOI) 10.1139/x95-049
Page Count 6
Web of Science Category Forestry
Subject Category Forestry
Document Delivery Number QY716
Unique Article Identifier WOS:A1995QY71600009
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