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Publication Type J
Authors Li, X. W., F. W. Wang, D. Q. Sun, N. Wang, Y. Y. Dong, W. C. Liu, X. M. Liu, N. Yao, H. Chen, M. H. Chi, S. H. Ji, S. J. Wang and H. Y. Li
Title Cloning and characterization of SucNHX1, a novel vacular Na+/H+ antiporter from the halophyte Suaeda corniculata that enhances the saline-alkali tolerance in Arabidopsis by its overexpression
Source Plant Cell Tissue and Organ Culture
Author Keywords Functional complementation Saline-alkali stress tolerance Suaeda corniculata Transgenic Arabidopsis Vacuolar Na+/H+ antiporter improves salt tolerance saccharomyces-cerevisiae mediated transformation bacillus-subtilis ph homeostasis gene plants thaliana sodium exchangers
Abstract Vacuolar Na+/H+ antiporters play central roles in plant saline (NaCl) stress response. However, the functional characterizations of antiporters involved in plant saline-alkali (Na2CO3/NaHCO3) tolerance are limited. In this study, SucNHX1, a Na+/H+ antiporter gene was isolated from Suaeda corniculata, a halophyte native to the saline-alkali soil, and further studied in transgenic Arabidopsis thaliana. The SucNHX1 gene contains 2123 nucleotides with an open reading frame of 1665 nucleotides (GenBank accession number: DQ512716), encoding a polypeptide of 554 amino acids with a predicted molecular mass of 61.2 kDa. A northern blot analysis indicated that the enhanced mRNA accumulation of SucNHX1 was induced by sodium carbonate (Na2CO3) rather than by NaCl, polyethylene glycol or abscisic acid in S. corniculata seedlings. SucNHX1 was localized in membrane, nucleus and cytoplasm when it was transiently expressed in tobacco mesophyll cells. The expression of SucNHX1 in salt sensitive yeast mutant Delta ena Delta nhx1 which lost of yeast vacuolar Na+/H+ antiporter ScNHX1 showed functional complementation. The transgenic Arabidopsis overexpressing SucNHX1 had higher Na2CO3 and NaCl tolerance levels than the wild-type plants. The expression patern of SucNHX1 under Na2CO3 and high NaCl stress in transgenic plants showed that SucNHX1 higher expressed under stress condition than normal control. The analysis of ion contents indicated that under Na2CO3 and NaCl conditions, there were more Na+ in the roots and higher K+/Na+ ratio in the leaves in the transgenic plants than in the wild-type plants. Thus, SucNHX1 may play a significant role in plant saline-alkali tolerance.
Author Address [Li, Xiaowei; Wang, Fawei; Sun, Daqian; Wang, Nan; Dong, Yuanyuan; Liu, Weican; Liu, Xiuming; Yao, Na; Chen, Huan; Chi, Menghan; Ji, Shuhan; Wang, Shijin; Li, Haiyan] Jilin Agr Univ, Chinese Minist Educ Bioreanactor & Pharmaceut Dev, Engn Res Ctr, Coll Life Sci, Changchun 130118, Jilin, Peoples R China. Li, HY (reprint author), Jilin Agr Univ, Chinese Minist Educ Bioreanactor & Pharmaceut Dev, Engn Res Ctr, Coll Life Sci, Changchun 130118, Jilin, Peoples R China. hyli99@163.com
ISSN 0167-6857
ISBN 0167-6857
29-Character Source Abbreviation Plant Cell Tissue Organ Cult.
Publication Date Sep
Year Published 2018
Volume 134
Issue 3
Beginning Page 395-407
Digital Object Identifier (DOI) 10.1007/s11240-018-1430-9
Unique Article Identifier WOS:000441559700005
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