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Publication Type J
Authors Mukhtar, S., S. Mehnaz, M. S. Mirza, B. S. Mirza and K. A. Malik
Title Diversity of Bacillus-like bacterial community in the rhizospheric and non-rhizospheric soil of halophytes (Salsola stocksii and Atriplex amnicola), and characterization of osmoregulatory genes in halophilic Bacilli
Source Canadian Journal of Microbiology
Author Keywords halophilic Bacilli pyrosequencing osmoregulatory genes Salsola stocksii Atriplex amnicola neighbor-joining method organic-matter microbiome sediments salinity enzymes osmoadaptation identification taxonomy betaine
Abstract Salinity is one of the major abiotic stresses; a total of 3% of the world's land mass is affected by salinity. Approximately 6.3 million hectares of land in Pakistan is affected by salinity to varying degrees, and most of the areas are arid to semiarid with low annual precipitation. The aim of the present study is to identify and characterize Bacillus and Bacillus-derived bacterial genera from the rhizospheric and non-rhizospheric soil samples from the Khewra Salt Mine, Pakistan, by using culture-independent and -dependent methods. Seven Bacillus-like bacterial genera, Bacillus, Halobacillus, Virgibacillus, Brevibacillus, Paenibacillus, Tumebacillus, and Lysinibacillus, were detected by using pyrosequencing analysis, whereas only four genera, Bacillus, Halobacillus, Oceanobacillus, and Virgibacillus, were identified by culture-dependent methods. Most of the Bacillus-like isolates identified in this study were moderately halophilic, alkaliphilic, and mesophilic bacteria and were considered a good source of hydrolytic enzymes because of their ability to degrade proteins, carbohydrates, and lipids. Eight Bacillus-like strains from the genera Bacillus, Halobacillus, Oceanobacillus, and Virgibacillus showed positive results for the presence of ectABC gene cluster (ectoine), six strains could synthesize betaine from choline, and six strains tested positive for the synthesis of proline from either glutamate or ornithine by using proline dehydrogenase enzyme.
Author Address [Mukhtar, Salma; Mehnaz, Samina; Malik, Kauser Abdulla] Forman Christian Coll, Dept Biol Sci, Ferozepur Rd, Lahore 54600, Pakistan. [Mukhtar, Salma] Univ Calif Los Angeles, Mol Cell & Dev Biol, 621 Charles Young Dr South, Los Angeles, CA 90095 USA. [Mirza, Muhammad Sajjad] NIBGE, Environm Biotechnol Div, Jhang Rd, Faisalabad, Pakistan. [Mirza, Babur Saeed] Missouri State Univ, 901 S Natl Ave, Springfield, MO 65897 USA. Malik, KA (reprint author), Forman Christian Coll, Dept Biol Sci, Ferozepur Rd, Lahore 54600, Pakistan. kausermalik@fccollege.edu.pk
ISSN 0008-4166
ISBN 0008-4166
29-Character Source Abbreviation Can. J. Microbiol.
Publication Date Aug
Year Published 2018
Volume 64
Issue 8
Beginning Page 567-579
Digital Object Identifier (DOI) 10.1139/cjm-2017-0544
Unique Article Identifier WOS:000440155000006
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