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Publication Type J
Authors Yao, L. R., J. C. Wang, B. C. Li, Y. X. Meng, X. L. Ma, E. J. Si, P. R. Ren, K. Yang, X. W. Shang and H. J. Wang
Title Transcriptome sequencing and comparative analysis of differentially-expressed isoforms in the roots of Halogeton glomeratus under salt stress
Source Gene
Author Keywords Halogeton glomeratus Roots Transcriptome sequencing Isoforms Salt tolerance Transporter halophyte suaeda maritima na+/h+ antiporter gene arabidopsis-thaliana salinity tolerance thellungiella-halophila puccinellia tenuiflora k+ homeostasis rna-seq transport plants
Abstract Although Halogeton glomeratus (H. glomeratus) has been confirmed to have a unique mechanism to regulate Na+ efflux from the cytoplasm and compartmentalize Na+ into leaf vacuoles, little is known about the salt tolerance mechanisms of roots under salinity stress. In the present study, transcripts were sequenced using the BGISEQ-500 sequencing platform (BGI, Wuhan, China). After quality control, approximately 24.08 million clean reads were obtained and the average mapping ratio to the reference gene was 70.00%. When comparing salt-treated samples with the control, a total of 550, 590, 1411 and 2063 DEIs were identified at 2, 6, 24 and 72 h, respectively. Numerous differentially-expressed isoforms that play important roles in response and adaptation to salt condition are related to metabolic processes, cellular processes, single-organism processes, localization, biological regulation, responses to stimulus, binding, catalytic activity and transporter activity. Fifty-eight salt-induced isoforms were common to different stages of salt stress; most of these DEIs were related to signal transduction and transporters, which maybe the core isoforms regulating Na+ uptake and transport in the roots of H. glomeratus. The expression patterns of 18 DEIs that were detected by quantitative real-time polymerase chain reaction were consistent with their respective changes in transcript abundance as identified by RNA-Seq technology. The present study thoroughly explored potential isoforms involved in salt tolerance on H. glomeratus roots at five time points. Our results may serve as an important resource for the H. glomeratus research community, improving our understanding of salt tolerance in halophyte survival under high salinity stress.
Author Address [Yao, Lirong; Wang, Juncheng; Li, Baochun; Meng, Yaxiong; Ma, Xiaole; Si, Erjing; Ren, Panrong; Yang, Ke; Wang, Huajun] Gansu Key Lab Crop Improvement & Germplasm Enhanc, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou, Gansu, Peoples R China. [Yao, Lirong; Wang, Juncheng; Meng, Yaxiong; Ma, Xiaole; Si, Erjing; Ren, Panrong; Yang, Ke; Shang, Xunwu; Wang, Huajun] Gansu Agr Univ, Coll Agron, Lanzhou, Gansu, Peoples R China. [Li, Baochun] Gansu Agr Univ, Coll Life Sci & Technol, Dept Bot, Lanzhou, Gansu, Peoples R China. Wang, HJ (reprint author), Gansu Agr Univ, Coll Agron, Lanzhou, Gansu, Peoples R China. whuajun@yahoo.com
ISSN 0378-1119
ISBN 0378-1119
29-Character Source Abbreviation Gene
Publication Date Mar
Year Published 2018
Volume 646
Beginning Page 159-168
Digital Object Identifier (DOI) 10.1016/j.gene.2017.12.058
Unique Article Identifier WOS:000425080000020
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