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Publication Type J
Authors Gao, H. J., X. P. Lue, L. Zhang, Y. Qiao, Q. Zhao, Y. P. Wang, M. F. Li and J. L. Zhang
Title Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress
Source International Journal of Molecular Sciences
Author Keywords Haloxylon ammodendron osmotic stress molecular responses regulation pathways physiological analysis osmoprotectants enhances drought resistance abiotic stress transgenic tobacco salt tolerance abscisic-acid boea-hygrometrica improves drought gene-expression reactive oxygen desert poplar
Abstract Haloxylon ammodendron, a perennial xero-halophyte, is an essential species for investigating the effects of drought on desert tree. To gain a comprehensive knowledge on the responses of H. ammodendron to drought stress, we specially performed the molecular and physiological analysis of H. ammodendron in response to -0.75 MPa osmotic stress for six and 24 h in lab condition via RNA-seq and digital gene expression (DGE). In total, 87,109 unigenes with a mean length of 680 bp and 13,486 potential simple sequence repeats (SSRs) were generated, and 3353 differentially expressed genes (DEGs) in shoots and 4564 in roots were identified under stress. These DEGs were mainly related to ion transporters, signal transduction, ROS-scavenging, photosynthesis, cell wall organization, membrane stabilization and hormones. Moreover, the physiological changes of inorganic ions and organic solute content, peroxidase (POD) activity and osmotic potential were in accordance with dynamic transcript profiles of the relevant genes. In this study, a detailed investigation of the pathways and candidate genes identified promote the research on the molecular mechanisms of abiotic stress tolerance in the xero-halophytic species. Our data provides valuable genetic resources for future improvement of forage and crop species for better adaptation to abiotic stresses.
Author Address [Gao, Hui-Juan; Lue, Xin-pei; Zhang, Ling; Zhao, Qi; Wang, Yong-Ping; Zhang, Jin-Lin] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China. [Qiao, Yan] Longdong Univ, Coll Agr & Forestry, Qingyang 745000, Peoples R China. [Li, Meng-Fei; Zhang, Jin-Lin] Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China. Zhang, JL (reprint author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China.; Zhang, JL (reprint author), Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China. gaohj15@lzu.edu.cn; lvxp17@lzu.edu.cn; lzhang12@lzu.edu.cn; yanqiao@ldxy.edu.cn; zhaoq@lzu.edu.cn; wangyp16@lzu.edu.cn; lmf@gsau.edu.cn; jlzhang@lzu.edu.cn
ISSN 1422-0067
ISBN 1422-0067
29-Character Source Abbreviation Int. J. Mol. Sci.
Publication Date Jan
Year Published 2018
Volume 19
Issue 1
Digital Object Identifier (DOI) 10.3390/ijms19010084
Unique Article Identifier WOS:000424407200082
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