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Publication Type J
Authors Meng, L; Zhang, L; Guo, Q; Li, SS; Mao, PC; Tian, XX
Author Full Name Meng, Lin; Zhang, Lin; Guo, Qiang; Li, Shan-Shan; Mao, Pei-Chun; Tian, Xiao-Xia
Title Cloning and Transformation of EeHKT1;4 Gene from Elytrigia elongata
Source PROTEIN AND PEPTIDE LETTERS
Language English
Document Type Article
Author Keywords Clone; EeHKT1;4 gene; Elytrigia elongata (Host) Nevski; plant expression vector; transformation; transgenic tobacco
Keywords Plus HKT TRANSPORTERS; SALT TOLERANCE; SODIUM EXCLUSION; CATION TRANSPORTER; KEY DETERMINANTS; SALINE SOILS; ORYZA-SATIVA; GRAIN-YIELD; DURUM-WHEAT; NA+ UPTAKE
Abstract The EeHKT1;4 gene was firstly cloned from Elytrigia elongata by RT-PCR technique with 1977 bp full-length cDNA encoding 1722 bp open reading frame (ORF) and 573 amino acids. The PCR fragment of EeHKT1;4 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-35S-EeHKT1;4-Nos. The vector was further transformed into the agrobacterium EHA105, and then EeHKT1;4 gene was transferred into tobacco by the Agrobaterium-mediated genetic transformation method. The results showed that the target gene was inserted into the genomes of tobacco and expressed. Therefore, the transgenic tobacco (T-0) plants overexpressing EeHKT1;4 gene were successfully obtained in this study. And EeHKT1;4 reduces Na+ concentration in the leaves of T-0 plants, thereby plays a central role in protecting plant leaves from salinity stress.
Author Address [Meng, Lin; Zhang, Lin; Guo, Qiang; Li, Shan-Shan; Mao, Pei-Chun; Tian, Xiao-Xia] Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China
Reprint Address Meng, L (reprint author), Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China.
E-mail Address menglin9599@sina.com
Funding Agency and Grant Number National Natural Science Foundation of China [31272489]; Scientific Innovation Ability Construction Project of Beijing Academy of Agriculture and Forestry Sciences (BAAFS) [KJCX20140103]
Funding Text This work was supported by National Natural Science Foundation of China (Grant No. 31272489) and Scientific Innovation Ability Construction Project of Beijing Academy of Agriculture and Forestry Sciences (BAAFS) (Grant No. KJCX20140103).
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Publisher BENTHAM SCIENCE PUBL LTD
Publisher City SHARJAH
Publisher Address EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES
ISSN 0929-8665
29-Character Source Abbreviation PROTEIN PEPTIDE LETT
ISO Source Abbreviation Protein Pept. Lett.
Year Published 2016
Volume 23
Issue 5
Beginning Page 488
Ending Page 494
Digital Object Identifier (DOI) 10.2174/0929866523666160314152116
Subject Category 7
Document Delivery Number Biochemistry & Molecular Biology
Unique Article Identifier Biochemistry & Molecular Biology
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