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Publication Type J
Authors Mello, Joao C. P.
Author Full Name Blainski, Andressa; Antonelli-Ushirobira, Tania M.; Godoy, Guilherme; Leite-Mello, Eneri V. S.; Mello, Joao C. P.
Title Pharmacognostic evaluation, and development and validation of a HPLC-DAD technique for gallocatechin and epigallocatechin in rhizomes from Limonium brasiliense
Source REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
Language English
Document Type Article
Author Keywords HPLC-DAD
Keywords Plus LIQUID-CHROMATOGRAPHY; TANNINS; LEAVES; RATS
Abstract Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, is a plant from the southern coast of Brazilian that is used for the treatment of premenstrual syndrome, menstrual disorders and genito-urinary infections. The aim of the present study was to determine the quality control parameters for rhizomes collected during different periods by pharmacopoeial and non-pharmacopoeial methods, and to develop and validate a HPLC-DAD method for quantitative control of marker substances. The measured parameters were: granulometric analysis (d(50)= 0.21-0.48 mm), loss on drying (11.1-12.4%), total ash (4.9-5.7%), dry residue by extraction with acetone: water (7: 3, v/v) (30.6-39.5%), total polyphenol content (8.5-15.8%), and chromatographic fingerprint by HPLC and TLC. Besides, the acetone: water (7: 3, v/v) extraction solvent in combination with a turbo-extractor, yielded the crude extract with a significant increase in tannins( F-4,F-20= 37.0, p < 0.001). The antioxidant potential of the crude acetone: water (7: 3, v/v) extract, as well as the ethyl acetate and water fractions obtained after the partition process was evaluated by DPPH and the results were, respectively: IC50 6.87, 5.91, and 6.92 mu g/ml. The validation parameters for the HPLC-DAD method showed adequate specificity, precision and accuracy. The gallo-and epigallocatechin contents were, respectively, 0.8-2.7% and 1.2-2.2%. These data contribute to analysis of the pharmacognostic quality control of the commonly used part from this species. (C)2017 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
Author Address [Blainski, Andressa; Antonelli-Ushirobira, Tania M.; Mello, Joao C. P.] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, Maringa, PR, Brazil; [Godoy, Guilherme] Univ Estadual Maringa, Fac Farm, Maringa, PR, Brazil; [Leite-Mello, Eneri V. S.] Univ Estadual Maringa, Dept Ciencias Morfol, Maringa, PR, Brazil
Reprint Address Mello, JCP (reprint author), Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, Maringa, PR, Brazil.
E-mail Address mello@uem.br
ResearcherID Number de Mello, Joao Carlos P/B-9418-2013
ORCID Number Mello, Joao Carlos Palazzo de/0000-0002-7532-3395
Funding Agency and Grant Number CNPq; CAPES; FINEP; Fundacao Araucaria; Instituto Nacional de Ciencia e Tecnologia para Inovacao Farmaceutica (INCT_if)
Funding Text authors thank A. Arantes for technical support during the experiments, Ache Laboratorios Farmaceuticos S.A. for providing the UV and HPLC instrumentation and Finzelberg GmbH & Co. KG for TLC instrumentation for analytical experiments. This study received support from CNPq, CAPES, FINEP, Fundacao Araucaria, and the Instituto Nacional de Ciencia e Tecnologia para Inovacao Farmaceutica (INCT_if).
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Cited Reference Count 36
Publisher SOC BRASILEIRA FARMACOGNOSIA
Publisher City CURITIBA-PR
Publisher Address C/O PROF CID AIMBIRE DE MORAES SANTOS, UFPR-LAB FARM, RUA PREF LOTHARIO MEISSNER, 632-JD BOTANICO, CURITIBA-PR, 80210-170, BRAZIL
ISSN 0102-695X
29-Character Source Abbreviation REV BRAS FARMACOGN
ISO Source Abbreviation Rev. Bras. Farmacogn.-Braz. J. Pharmacogn.
Publication Date MAR-APR
Year Published 2017
Volume 27
Issue 2
Beginning Page 162
Ending Page 169
Digital Object Identifier (DOI) 10.1016/j.bjp.2016.08.009
Page Count 8
Subject Category Chemistry, Medicinal; Pharmacology & Pharmacy
Unique Article Identifier WOS:000397844800005
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