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Publication Type J
Authors de Mello, Joao Carlos Palazzo
Author Full Name Blainski, Andressa; Lopes, Gisely Cristiny; de Mello, Joao Carlos Palazzo
Title Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium Brasiliense L.
Source MOLECULES
Language English
Document Type Article
Author Keywords Folin-Ciocalteu
Keywords Plus ANTIOXIDANT ACTIVITY; TANNINS; PROANTHOCYANIDINS; POLYPHENOLS; VALIDATION; LEAVES
Abstract Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Agency, ANVISA) has canceled the registration of these products, and their use was discontinued because of a lack of studies to characterize the plant raw material and ensure the effectiveness and safety of its use. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from L. brasiliense roots, by the UV/Vis spectrophotometric method. L. brasiliense roots were extracted in acetone: water (7: 3, v/v-10% w/v). The crude extract was used to develop a method for TP assay. The method was validated according to national and international guidelines. The optimum conditions for analysis time, wavelength, and standard substance were 30 min, 760 nm, and pyrogallol, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.
Author Address [Blainski, Andressa; Lopes, Gisely Cristiny; de Mello, Joao Carlos Palazzo] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, BR-87020900 Maringa, Parana, Brazil
Reprint Address de Mello, JCP (reprint author), Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, Av Colombo 5790, BR-87020900 Maringa, Parana, Brazil.
E-mail Address andressa_blainski@yahoo.com.br; giselycl@yahoo.com.br; mello@uem.br
ResearcherID Number de Mello, Joao Carlos P/B-9418-2013
ORCID Number Mello, Joao Carlos Palazzo de/0000-0002-7532-3395
Funding Agency and Grant Number CAPES; FINEP; CNPq; INCT_if; Fundacao Araucaria
Funding Text The authors are grateful for financial support from CAPES, FINEP, CNPq, INCT_if, and Fundacao Araucaria. The technical assistance provided by A. Arantes and C. R. Novello is gratefully acknowledged. Thanks are due to Janet W. Reid, JWR Associates, Trumansburg, New York, for English revision.
Cited References [Anonymous], 2010, FARMACOPEIA BRASILEI; Agencia Nacional de Vigilancia Sanitaria, 2003, 899 RE MIN SAUD AG N; Bruce P, 1998, MIKROCHIM ACTA, V128, P93, DOI 10.1007/BF01242196 ; Bueno FG, 2012, QUIM NOVA, V35, P822, DOI 10.1590/S0100-40422012000400031; Cardoso M. L. C., 1990, THESIS FEDERAL U RIO; Cicco N, 2009, MICROCHEM J, V91, P107, DOI 10.1016/j.microc.2008.08.011; Council of Europe, 2007, EUROPEAN PHARMACOPOE, pA286; Dias da Silva R.A, 1920, B ASS BRAS PHARM, V1, P4; Pelozo MID, 2008, BRAZ ARCH BIOL TECHN, V51, P447, DOI 10.1590/S1516-89132008000300002; Fenner R., 2006, REV BRAS CIENC FARM, V42, P269; Ferreira D, 2005, PHYTOCHEMISTRY, V66, P2216, DOI [10.1016/j.phytochem.2005.01.014, 10.1016/j.phytochem.2005.08.010]; Folin O, 1927, J BIOL CHEM, V73, P627; Glasl H., 1983, DTSCH APOTH ZTG, V123, P1979; Gottlieb OR, 2000, MEM I OSWALDO CRUZ, V95, P115, DOI 10.1590/S0074-02762000000100019; Gulcin I, 2004, FOOD CHEM, V87, P393, DOI 10.1016/j.foodchem.2003.12.008; International Conference on Harmonization, 2005, Q2B R1 GUID VAL AN P; Janhs R. T., 1976, TRIB PHARM, V44, P105; Lopes GC, 2009, CHROMATOGRAPHIA, V69, P175, DOI 10.1365/s10337-009-0990-6; Lopes Gisely Cristiny, 2010, Rev. bras. farmacogn., V20, P781, DOI 10.1590/S0102-695X2010000500022; Moller C, 2009, PHYTOCHEM ANALYSIS, V20, P231, DOI 10.1002/pca.1119; Moura T. F. A. L., 1984, THESIS FEDERAL U RIO; Murray AR, 2004, Z NATURFORSCH C, V59, P477; Okuda T, 2011, MOLECULES, V16, P2191, DOI 10.3390/molecules16032191; Ossipova S, 2001, PHYTOCHEM ANALYSIS, V12, P128, DOI 10.1002/pca.568; Pieroni LG, 2011, MOLECULES, V16, P9439, DOI 10.3390/molecules16119439; Ribani M, 2004, QUIM NOVA, V27, P771, DOI 10.1590/S0100-40422004000500017; Schofield P, 2001, ANIM FEED SCI TECH, V91, P21, DOI 10.1016/S0377-8401(01)00228-0; Singleton VL, 1999, METHOD ENZYMOL, V299, P152; Verza Simone Gasparin, 2007, Quím. Nova, V30, P815, DOI 10.1590/S0100-40422007000400011; Vogel A. I., 2002, QUIMICA ANAL QUANTIT, P462
Cited Reference Count 30
Times Cited 83
Total Times Cited Count (WoS, BCI, and CSCD) 87
Publisher MDPI AG
Publisher City BASEL
Publisher Address POSTFACH, CH-4005 BASEL, SWITZERLAND
29-Character Source Abbreviation MOLECULES
ISO Source Abbreviation Molecules
Publication Date JUN
Year Published 2013
Volume 18
Issue 6
Beginning Page 6852
Ending Page 6864
Digital Object Identifier (DOI) 10.3390/molecules18066852
Page Count 13
Subject Category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
Unique Article Identifier WOS:000320770800040
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