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Publication Type J
Authors Ben Mansour, R; Ksouri, WM; Cluzet, S; Krisa, S; Richard, T; Ksouri, R
Author Full Name Ben Mansour, Rim; Ksouri, Wided Megdiche; Cluzet, Stephanie; Krisa, Stephanie; Richard, Tristan; Ksouri, Riadh
Title Assessment of Antioxidant Activity and Neuroprotective Capacity on PC12 Cell Line of Frankenia thymifolia and Related Phenolic LC-MS/MS Identification
Source EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
Language English
Document Type Article
Keywords Plus ALZHEIMERS-DISEASE; LIPID-PEROXIDATION; MASS-SPECTROMETRY; OXIDATIVE STRESS; EXTRACTS; NEURONS; HPLC; L.; NEUROTOXICITY; STRAWBERRIES
Abstract This work aimed to investigate the richness of a Tunisian xerohalophyte Frankenia thymifolia aerial and root parts on phenolics and to evaluate the antioxidant and neuroprotective properties of this medicinal species. After fractionation using increasing and different solvent polarities, results displayed five fractions, where ethyl acetate (EtOAc) shoot and root fractions possess considerable total phenolic contents (221 and 308 mg of GAE/g of E, resp.) related to their important antioxidant activities such as ORAC (918 and 713 mg of TE/g of E), DPPH(282 and 821 mg of TE/g), and ABTS (778 and 1320 mg of TE/g) tests. Then, the identification of the main compounds by HPLC-DAD-ESI-MS and neuroprotective property of the most active fraction EtOAc were assessed. A total of 14 molecules were identified, which have been described for the first time in F. thymifolia. The major compounds identified were pinoresinol and kaempferol glycoside in aerial parts and gallic acid and ellagitannin in roots. Neuroprotective capacity against beta-amyloid (A beta) peptide induced toxicity in PC12 cells of EtOAc fraction showed a significant protective activity at lower concentration (25 and 50 mu M). The relevant antioxidant and neuroprotective activities of F. thymifolia EtOAc fraction corroborated their chemical compositions.
Author Address [Ben Mansour, Rim; Ksouri, Wided Megdiche; Ksouri, Riadh] Technopk Borj Cedria CBBC, Ctr Biotechnol, Lab Aromat & Med Plants, BP 901, Hammam Lif 2050, Tunisia; [Cluzet, Stephanie; Krisa, Stephanie; Richard, Tristan] Univ Bordeaux, ISVV, GESVAB EA3675, F-33882 Villenave Dornon, France
Reprint Address Ksouri, R (reprint author), Technopk Borj Cedria CBBC, Ctr Biotechnol, Lab Aromat & Med Plants, BP 901, Hammam Lif 2050, Tunisia.
E-mail Address ksouri.riadh@gmail.com
ORCID Number Megdiche-Ksouri, Wided/0000-0001-5497-0131; Richard, Tristan/0000-0002-5308-8697
Funding Agency and Grant Number Tunisian Ministry of Higher Education and Scientific Research [LR15CBBC06]
Funding Text This work was supported by the Tunisian Ministry of Higher Education and Scientific Research (LR15CBBC06).
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Cited Reference Count 46
Publisher HINDAWI LTD
Publisher City LONDON
Publisher Address ADAM HOUSE, 3RD FLR, 1 FITZROY SQ, LONDON, W1T 5HF, ENGLAND
ISSN 1741-427X
29-Character Source Abbreviation EVID-BASED COMPL ALT
ISO Source Abbreviation Evid.-based Complement Altern. Med.
Year Published 2016
Article Number 2843463
Digital Object Identifier (DOI) 10.1155/2016/2843463
Page Count 8
Web of Science Category Integrative & Complementary Medicine
Subject Category Integrative & Complementary Medicine
Document Delivery Number EA8PX
Unique Article Identifier WOS:000386900200001
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