eHALOPH - Halophytes Database

Publication Type	J
Authors	Zeng, HC; Deng, LH; Zhang, CF
Author Full Name	Zeng, Hui-Cai; Deng, Liu-Hong; Zhang, Chun-Fa
Title	Cloning of salt tolerance-related cDNAs from the mangrove plant Sesuvium portulacastrum L.
Source	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Language	English
Document Type	Article
Author Keywords	mangrove; salt tolerance-related cDNAs; Sesuvium portulacastrum; suppressive subtractive hybridization
Keywords Plus	WATER-DEFICIT; RESPONSES; STRESS; EXPRESSION; RICE
Abstract	In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant (Sesuvium portulacastrum L.), a subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the poly(A)(+)RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly(A)(+)RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant (LEA) protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.
Author Address	Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China; CATAS, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China
Reprint Address	Zhang, CF (reprint author), Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China.
E-mail Address	zhang_cs@hotmail.com
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Cited Reference Count	11
Times Cited	10
Total Times Cited Count (WoS, BCI, and CSCD)	14
Publisher	BLACKWELL PUBLISHING
Publisher City	OXFORD
Publisher Address	9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
ISSN	1672-9072
29-Character Source Abbreviation	J INTEGR PLANT BIOL
ISO Source Abbreviation	J. Integr. Plant Biol.
Publication Date	AUG
Year Published	2006
Volume	48
Issue	8
Beginning Page	952
Ending Page	957
Digital Object Identifier (DOI)	10.1111/j.1744-7909.2006.00287.x
Page Count	6
Web of Science Category	Biochemistry & Molecular Biology; Plant Sciences
Subject Category	Biochemistry & Molecular Biology; Plant Sciences
Document Delivery Number	070GW
Unique Article Identifier	WOS:000239510200010
Plants associated with this reference	Sesuvium portulacastrum