Publication Type |
J |
Authors |
Zeng, HC; Deng, LH; Zhang, CF |
Author Full Name |
Zeng, Hui-Cai; Deng, Liu-Hong; Zhang, Chun-Fa |
Title |
Cloning of salt tolerance-related cDNAs from the mangrove plant Sesuvium portulacastrum L. |
Source |
JOURNAL OF INTEGRATIVE PLANT BIOLOGY |
Language |
English |
Document Type |
Article |
Author Keywords |
mangrove; salt tolerance-related cDNAs; Sesuvium portulacastrum; suppressive subtractive hybridization |
Keywords Plus |
WATER-DEFICIT; RESPONSES; STRESS; EXPRESSION; RICE |
Abstract |
In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant (Sesuvium portulacastrum L.), a subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the poly(A)(+)RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly(A)(+)RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant (LEA) protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants. |
Author Address |
Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China; CATAS, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China |
Reprint Address |
Zhang, CF (reprint author), Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China. |
E-mail Address |
zhang_cs@hotmail.com |
Cited References |
BOYER JS, 1982, SCIENCE, V218, P443, DOI 10.1126/science.218.4571.443; Bray EA, 1997, TRENDS PLANT SCI, V2, P48, DOI 10.1016/S1360-1385(97)82562-9; Close TJ, 1996, PHYSIOL PLANTARUM, V97, P795, DOI 10.1111/j.1399-3054.1996.tb00546.x; Hasegawa PM, 2000, ANNU REV PLANT PHYS, V51, P463, DOI 10.1146/annurev.arplant.51.1.463; Ingram J, 1996, ANNU REV PLANT PHYS, V47, P377, DOI 10.1146/annurev.arplant.47.1.377; MOONS A, 1995, PLANT PHYSIOL, V107, P177, DOI 10.1104/pp.107.1.177; TOMLINSON PB, 1986, BOTANY MANGROVES; Xu DP, 1996, PLANT PHYSIOL, V110, P249; Yamada A, 2002, PLANT CELL PHYSIOL, V43, P903, DOI 10.1093/pcp/pcf108; Yamada A, 2002, PLANT CELL PHYSIOL, V43, P1043, DOI 10.1093/pcp/pcf120; Zhu JK, 2002, ANNU REV PLANT BIOL, V53, P247, DOI 10.1146/annurev.arplant.53.091401.143329 |
Cited Reference Count |
11 |
Times Cited |
10 |
Total Times Cited Count (WoS, BCI, and CSCD) |
14 |
Publisher |
BLACKWELL PUBLISHING |
Publisher City |
OXFORD |
Publisher Address |
9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND |
ISSN |
1672-9072 |
29-Character Source Abbreviation |
J INTEGR PLANT BIOL |
ISO Source Abbreviation |
J. Integr. Plant Biol. |
Publication Date |
AUG |
Year Published |
2006 |
Volume |
48 |
Issue |
8 |
Beginning Page |
952 |
Ending Page |
957 |
Digital Object Identifier (DOI) |
10.1111/j.1744-7909.2006.00287.x |
Page Count |
6 |
Web of Science Category |
Biochemistry & Molecular Biology; Plant Sciences |
Subject Category |
Biochemistry & Molecular Biology; Plant Sciences |
Document Delivery Number |
070GW |
Unique Article Identifier |
WOS:000239510200010
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Plants associated with this reference |
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