Loading content, please wait..
loading..
Logo
Version 3.20
or
Publication Type J
Authors Zeng, HC; Deng, LH; Zhang, CF
Author Full Name Zeng, Hui-Cai; Deng, Liu-Hong; Zhang, Chun-Fa
Title Cloning of salt tolerance-related cDNAs from the mangrove plant Sesuvium portulacastrum L.
Source JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Language English
Document Type Article
Author Keywords mangrove; salt tolerance-related cDNAs; Sesuvium portulacastrum; suppressive subtractive hybridization
Keywords Plus WATER-DEFICIT; RESPONSES; STRESS; EXPRESSION; RICE
Abstract In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant (Sesuvium portulacastrum L.), a subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the poly(A)(+)RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly(A)(+)RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant (LEA) protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.
Author Address Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China; CATAS, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China
Reprint Address Zhang, CF (reprint author), Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China.
E-mail Address zhang_cs@hotmail.com
Cited References BOYER JS, 1982, SCIENCE, V218, P443, DOI 10.1126/science.218.4571.443; Bray EA, 1997, TRENDS PLANT SCI, V2, P48, DOI 10.1016/S1360-1385(97)82562-9; Close TJ, 1996, PHYSIOL PLANTARUM, V97, P795, DOI 10.1111/j.1399-3054.1996.tb00546.x; Hasegawa PM, 2000, ANNU REV PLANT PHYS, V51, P463, DOI 10.1146/annurev.arplant.51.1.463; Ingram J, 1996, ANNU REV PLANT PHYS, V47, P377, DOI 10.1146/annurev.arplant.47.1.377; MOONS A, 1995, PLANT PHYSIOL, V107, P177, DOI 10.1104/pp.107.1.177; TOMLINSON PB, 1986, BOTANY MANGROVES; Xu DP, 1996, PLANT PHYSIOL, V110, P249; Yamada A, 2002, PLANT CELL PHYSIOL, V43, P903, DOI 10.1093/pcp/pcf108; Yamada A, 2002, PLANT CELL PHYSIOL, V43, P1043, DOI 10.1093/pcp/pcf120; Zhu JK, 2002, ANNU REV PLANT BIOL, V53, P247, DOI 10.1146/annurev.arplant.53.091401.143329
Cited Reference Count 11
Times Cited 10
Total Times Cited Count (WoS, BCI, and CSCD) 14
Publisher BLACKWELL PUBLISHING
Publisher City OXFORD
Publisher Address 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
ISSN 1672-9072
29-Character Source Abbreviation J INTEGR PLANT BIOL
ISO Source Abbreviation J. Integr. Plant Biol.
Publication Date AUG
Year Published 2006
Volume 48
Issue 8
Beginning Page 952
Ending Page 957
Digital Object Identifier (DOI) 10.1111/j.1744-7909.2006.00287.x
Page Count 6
Web of Science Category Biochemistry & Molecular Biology; Plant Sciences
Subject Category Biochemistry & Molecular Biology; Plant Sciences
Document Delivery Number 070GW
Unique Article Identifier WOS:000239510200010
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed