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| Publication Type | J |
| Authors | Shi, HT; Jiang, C; Ye, TT; Tan, DX; Reiter, RJ; Zhang, H; Liu, RY; Chan, ZL |
| Author Full Name | Shi, Haitao; Jiang, Chuan; Ye, Tiantian; Tan, Dun-Xian; Reiter, Russel J.; Zhang, Heng; Liu, Renyi; Chan, Zhulong |
| Title | Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by exogenous melatonin |
| Source | JOURNAL OF EXPERIMENTAL BOTANY |
| Language | English |
| Document Type | Article |
| Author Keywords | Abiotic stress; antioxidant; bermudagrass; melatonin; metabolites; reactive oxygen species; transcriptomic |
| Keywords Plus | SEROTONIN N-ACETYLTRANSFERASE; CHROMATOGRAPHY-MASS SPECTROMETRY; LATERAL ROOT-FORMATION; GENE-EXPRESSION DATA; CUCUMIS-SATIVUS L.; MICRO-TOM TOMATO; ARABIDOPSIS-THALIANA; OXIDATIVE STRESS; MALUS-HUPEHENSIS; SEEDLING GROWTH |
| Abstract | Melatonin (N-acetyl-5-methoxytryptamine), a well-known animal hormone, is also involved in plant development and abiotic stress responses. In this study, it is shown that exogenous application of melatonin conferred improved salt, drought, and cold stress resistances in bermudagrass. Moreover, exogenous melatonin treatment alleviated reactive oxygen species (ROS) burst and cell damage induced by abiotic stress; this involved activation of several antioxidants. Additionally, melatonin-pre-treated plants exhibited higher concentrations of 54 metabolites, including amino acids, organic acids, sugars, and sugar alcohols, than non-treated plants under abiotic stress conditions. Genome-wide transcriptomic profiling identified 3933 transcripts (2361 up-regulated and 1572 down-regulated) that were differentially expressed in melatonin-treated plants versus controls. Pathway and gene ontology (GO) term enrichment analyses revealed that genes involved in nitrogen metabolism, major carbohydrate metabolism, tricarboxylic acid (TCA)/org transformation, transport, hormone metabolism, metal handling, redox, and secondary metabolism were over-represented after melatonin pre-treatment. Taken together, this study provides the first evidence of the protective roles of exogenous melatonin in the bermudagrass response to abiotic stresses, partially via activation of antioxidants and modulation of metabolic homeostasis. Notably, metabolic and transcriptomic analyses showed that the underlying mechanisms of melatonin could involve major reorientation of photorespiratory and carbohydrate and nitrogen metabolism. |
| Author Address | [Shi, Haitao; Ye, Tiantian; Chan, Zhulong] Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China; [Jiang, Chuan; Zhang, Heng; Liu, Renyi] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China; [Jiang, Chuan; Ye, Tiantian] Univ Chinese Acad Sci, Beijing 100039, Peoples R China; [Tan, Dun-Xian; Reiter, Russel J.] Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA |
| Reprint Address | Zhang, H (reprint author), Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China. |
| E-mail Address | hengzhang@gmail.com; ryliu@sibs.ac.cn; zhulongch@wbgcas.cn |
| ORCID Number | Zhang, Heng/0000-0002-1541-3890; Chan, Zhulong/0000-0002-1933-6837 |
| Funding Agency and Grant Number | Knowledge Innovative Key Program of the Chinese Academy of Sciences [55Y455446O0544]; Youth Innovation Promotion Association of the Chinese Academy of Sciences [29Y429371O0437]; Chinese Academy of Sciences; 'Hundred Talents Program' of the Chinese Academy of Sciences [54Y154761O01076, 29Y329631O0263] |
| Funding Text | We thank Professor Pingfang Yang, Dr Yanqiong Jiang, and Dr Longxin Hu for their help with this study. This research was supported by the Knowledge Innovative Key Program of the Chinese Academy of Sciences (No. 55Y455446O0544), Youth Innovation Promotion Association of the Chinese Academy of Sciences (No. 29Y429371O0437) to HS, by funding from the Chinese Academy of Sciences to RL, and by 'the Hundred Talents Program' of the Chinese Academy of Sciences (Nos 54Y154761O01076 and 29Y329631O0263) to ZC. HS designed and performed the experiments, analysed the data, and wrote and revised the manuscript; CJ analysed the transcriptomic data; TY performed the metabolic experiment; HZ performed the transcriptomic experiment; RL analysed the transcriptomic data and revised the manuscript; DT and RR revised the manuscript; ZC designed the experiments and revised the manuscript; and all authors approved the manuscript. |
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| Cited Reference Count | 90 |
| Times Cited | 67 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 67 |
| Publisher | OXFORD UNIV PRESS |
| Publisher City | OXFORD |
| Publisher Address | GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND |
| ISSN | 0022-0957 |
| 29-Character Source Abbreviation | J EXP BOT |
| ISO Source Abbreviation | J. Exp. Bot. |
| Publication Date | FEB |
| Year Published | 2015 |
| Volume | 66 |
| Issue | 3 |
| Beginning Page | 681 |
| Ending Page | 694 |
| Digital Object Identifier (DOI) | 10.1093/jxb/eru373 |
| Page Count | 14 |
| Web of Science Category | Plant Sciences |
| Subject Category | Plant Sciences |
| Document Delivery Number | CE2RD |
| Unique Article Identifier | WOS:000351662300006 |
| Plants associated with this reference |