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Publication Type J
Authors Piro, A; Marin-Guirao, L; Serra, IA; Spadafora, A; Sandoval-Gil, JM; Bemardeau-Esteller, J; Femandez, JMR; Mazzuca, S
Author Full Name Piro, Amalia; Marin-Guirao, Lazaro; Serra, Ilia A.; Spadafora, Antonia; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.; Mazzuca, Silvia
Title The modulation of leaf metabolism plays a role in salt tolerance of Cymodocea nodosa exposed to hypersaline stress in mesocosms
Source FRONTIERS IN PLANT SCIENCE
Language English
Document Type Article
Author Keywords seagrasses; leaf proteomics; hypersaline; mesocosm
Keywords Plus SEAGRASS POSIDONIA-OCEANICA; REACTION-CENTER-II; PHOTOSYSTEM-II; D1 PROTEIN; SALINITY FLUCTUATION; PROTEOMIC ANALYSIS; MASS-SPECTROMETRY; ATPASE ACTIVITY; LIGHT; PLANT
Abstract Applying proteomics, we tested the physiological responses of the euryhaline seagrass Cymodocea nodosa to deliberate manipulation of salinity in a mesocosm system. Plants were subjected to a chronic hypersaline condition (43 psu) to compare protein expression and plant photochemistry responses after 15 and 30 days of exposure with those of plants cultured under normal/ambient saline conditions (37 psu). Results showed a general decline in the expression level of leaf proteins in hypersaline stressed plants, with more intense reductions after long-lasting exposure. Specifically, the carbon-fixing enzyme RuBisCo displayed a lower accumulation level in stressed plants relative to controls. In contrast, the key enzymes involved in the regulation of glycolysis, cytosolic glyceraldehyde-3-phosphate dehydrogenase, enolase 2 and triose-phosphate isomerase, showed significantly higher accumulation levels. These responses suggested a shift in carbon metabolism in stressed plants. Hypersaline stress also induced a significant alteration of the photosynthetic physiology of C. nodosa by means of a downregulation in structural proteins and enzymes of both PSII and PSI. However we found an over-expression of the cytochrome b559 alpha subunit of the PSII initial complex, which is a receptor for the PSII core proteins involved in biogenesis or repair processes and therefore potentially involved in the absence of effects at the photochemical level of stressed plants. As expected hypersalinity also affects vacuolar metabolism by increasing the leaf cell turgor pressure and enhancing the up-take of Na+ by overaccumulating the tonoplast specific intrinsic protein pyrophosphate-energized inorganic pyrophosphatase (H(+)-PPase) coupled to the Na+/H+-antiporter. The modulation of carbon metabolism and the enhancement of vacuole capacity in Na+ sequestration and osmolarity changes are discussed in relation to salt tolerance of C. nodosa.
Author Address [Piro, Amalia; Serra, Ilia A.; Spadafora, Antonia; Mazzuca, Silvia] Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, I-87036 Arcavacata Di Rende, Italy; [Marin-Guirao, Lazaro; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.] Spanish Inst Oceanog, Oceanog Ctr Murcia, Murcia, Spain
Reprint Address Mazzuca, S (reprint author), Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, Ponte Bucci 12C, I-87036 Arcavacata Di Rende, Italy.
E-mail Address silvia.mazzuca@unical.it
ResearcherID Number MarAn-Guirao, LAzaro/A-3481-2013
ORCID Number MarAn-Guirao, LAzaro/0000-0001-6240-8018; Mazzuca, Silvia/0000-0003-4648-2271; PIRO, Amalia/0000-0002-6296-621X
Funding Agency and Grant Number COST Action
Funding Text The authors are grateful to the COST Action E50906
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Cited Reference Count 56
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 7
Publisher FRONTIERS RESEARCH FOUNDATION
Publisher City LAUSANNE
Publisher Address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date JUN 26
Year Published 2015
Volume 6
Article Number 464
Digital Object Identifier (DOI) 10.3389/fpls.2015.00464
Page Count 12
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number CN4RD
Unique Article Identifier WOS:000358417000001
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