Loading content, please wait..
Version 3.24
Publication Type J
Authors Piro, A; Marin-Guirao, L; Serra, IA; Spadafora, A; Sandoval-Gil, JM; Bemardeau-Esteller, J; Femandez, JMR; Mazzuca, S
Author Full Name Piro, Amalia; Marin-Guirao, Lazaro; Serra, Ilia A.; Spadafora, Antonia; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.; Mazzuca, Silvia
Title The modulation of leaf metabolism plays a role in salt tolerance of Cymodocea nodosa exposed to hypersaline stress in mesocosms
Language English
Document Type Article
Author Keywords seagrasses; leaf proteomics; hypersaline; mesocosm
Abstract Applying proteomics, we tested the physiological responses of the euryhaline seagrass Cymodocea nodosa to deliberate manipulation of salinity in a mesocosm system. Plants were subjected to a chronic hypersaline condition (43 psu) to compare protein expression and plant photochemistry responses after 15 and 30 days of exposure with those of plants cultured under normal/ambient saline conditions (37 psu). Results showed a general decline in the expression level of leaf proteins in hypersaline stressed plants, with more intense reductions after long-lasting exposure. Specifically, the carbon-fixing enzyme RuBisCo displayed a lower accumulation level in stressed plants relative to controls. In contrast, the key enzymes involved in the regulation of glycolysis, cytosolic glyceraldehyde-3-phosphate dehydrogenase, enolase 2 and triose-phosphate isomerase, showed significantly higher accumulation levels. These responses suggested a shift in carbon metabolism in stressed plants. Hypersaline stress also induced a significant alteration of the photosynthetic physiology of C. nodosa by means of a downregulation in structural proteins and enzymes of both PSII and PSI. However we found an over-expression of the cytochrome b559 alpha subunit of the PSII initial complex, which is a receptor for the PSII core proteins involved in biogenesis or repair processes and therefore potentially involved in the absence of effects at the photochemical level of stressed plants. As expected hypersalinity also affects vacuolar metabolism by increasing the leaf cell turgor pressure and enhancing the up-take of Na+ by overaccumulating the tonoplast specific intrinsic protein pyrophosphate-energized inorganic pyrophosphatase (H(+)-PPase) coupled to the Na+/H+-antiporter. The modulation of carbon metabolism and the enhancement of vacuole capacity in Na+ sequestration and osmolarity changes are discussed in relation to salt tolerance of C. nodosa.
Author Address [Piro, Amalia; Serra, Ilia A.; Spadafora, Antonia; Mazzuca, Silvia] Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, I-87036 Arcavacata Di Rende, Italy; [Marin-Guirao, Lazaro; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.] Spanish Inst Oceanog, Oceanog Ctr Murcia, Murcia, Spain
Reprint Address Mazzuca, S (reprint author), Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, Ponte Bucci 12C, I-87036 Arcavacata Di Rende, Italy.
E-mail Address silvia.mazzuca@unical.it
ResearcherID Number MarAn-Guirao, LAzaro/A-3481-2013
ORCID Number MarAn-Guirao, LAzaro/0000-0001-6240-8018; Mazzuca, Silvia/0000-0003-4648-2271; PIRO, Amalia/0000-0002-6296-621X
Funding Agency and Grant Number COST Action
Funding Text The authors are grateful to the COST Action E50906
Cited References ADAMS JB, 1994, AQUAT BOT, V47, P341, DOI 10.1016/0304-3770(94)90063-9; ADIR N, 1990, J BIOL CHEM, V265, P12563; Marin-Guirao L., 2011, ESTUAR COAST SHELF S, V92, P286, DOI [10.1016/j.ecss.2011.01.003, DOI 10.1016/J.ECSS.2011.01.003]; Barbourina O., 2000, ANN BOT, V85, P759, DOI 10.1006/anbo.2000.1136; BEER S, 1980, J EXP BOT, V31, P1019, DOI 10.1093/jxb/31.4.1019; BEHBOUDIAN MH, 1986, SCI HORTIC-AMSTERDAM, V28, P105, DOI 10.1016/0304-4238(86)90130-5; BISSON MA, 1995, NATURWISSENSCHAFTEN, V82, P461, DOI 10.1007/BF01131597; Boudouresque F. C., 2009, BOT MAR, V52, P395, DOI 10.1515/BOT.2009.057; Coors A, 2006, ECOTOXICOLOGY, V15, P583, DOI 10.1007/s10646-006-0095-z; Dattolo E, 2014, MAR ENVIRON RES, V101, P225, DOI 10.1016/j.marenvres.2014.07.010; Dattolo E, 2013, FRONT PLANT SCI, V4, DOI 10.3389/fpls.2013.00195; Debez A, 2006, ENVIRON EXP BOT, V57, P285, DOI 10.1016/j.envexpbot.2005.06.009; Drapier D, 2007, EMBO J, V26, P3581, DOI 10.1038/sj.emboj.7601802; DREW EA, 1978, J EXP MAR BIOL ECOL, V31, P173, DOI 10.1016/0022-0981(78)90128-4; Elias JE, 2007, NAT METHODS, V4, P207, DOI 10.1038/nmeth1019; Epimashko S, 2006, PLANTA, V224, P944, DOI 10.1007/s00425-006-0265-5; Fenyo D, 2010, METHODS MOL BIOL, V673, P189, DOI 10.1007/978-1-60761-842-3_11; Fukuda A, 2004, J EXP BOT, V55, P585, DOI 10.1093/jxb/erh070; Garrote-Moreno A, 2014, MAR POLLUT BULL, V81, P61, DOI 10.1016/j.marpolbul.2014.02.019; Kahn AE, 2006, J EXP MAR BIOL ECOL, V335, P1, DOI 10.1016/j.jembe.2006.02.011; Kirst C.O., 1989, ANNU REV PLANT PHYS, V40, P21; Koch MS, 2007, AQUAT BOT, V86, P14, DOI 10.1016/j.aquabot.2006.08.003; KUO J, 2000, BIOL MARINA MEDITERR, V7, P3; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Marin-Guirao L, 2013, AQUAT BOT, V109, P14, DOI 10.1016/j.aquabot.2013.03.006; Mazzuca S., 2009, AQUAT BOT, V91, P117, DOI [10.1016/j.aquabot.2009.03.007, DOI 10.1016/J.AQUABOT.2009.03.007]; Mazzuca S, 2013, FRONT PLANT SCI, V4, DOI 10.3389/fpls.2013.00038; MONTAGUE CL, 1993, ESTUARIES, V16, P703, DOI 10.2307/1352429; Morais F, 1998, J BIOL CHEM, V273, P29315, DOI 10.1074/jbc.273.45.29315; Muller B, 1999, PLANT CELL, V11, P2365, DOI 10.1105/tpc.11.12.2365; Muramatsu Y, 2002, PLANT CELL PHYSIOL, V43, P1137, DOI 10.1093/pcp/pcf139; Portillo E, 2014, MAR POLLUT BULL, V80, P222, DOI 10.1016/j.marpolbul.2013.12.048; Procaccini G., 2003, WORLD ATLAS SEAGRASS, P48; Rott M, 2011, PLANT CELL, V23, P304, DOI 10.1105/tpc.110.079111; Ruiz JM, 2009, BOT MAR, V52, P459, DOI 10.1515/BOT.2009.051; Sanchez-Lizaso JL, 2008, DESALINATION, V221, P602, DOI 10.1016/j.desal.2007.01.119; Sandoval-Gil JM, 2012, ESTUAR COAST SHELF S, V115, P260, DOI 10.1016/j.ecss.2012.09.008; Sandoval-Gil JM, 2014, MAR ENVIRON RES, V95, P39, DOI 10.1016/j.marenvres.2013.12.011; Schreiber U., 2004, CHLOROPHYLL FLUORESC, P279, DOI DOI 10.1007/978-1-4020-3218-9_11; Serra IA, 2013, AQUAT BOT, V104, P213, DOI 10.1016/j.aquabot.2011.05.008; Serra I. A., 2011, SEAGRASS ECOLOGY USE, P71; Serra IA, 2012, MAR BIOL, V159, P1269, DOI 10.1007/s00227-012-1907-8; Spadafora A, 2008, PLANT BIOSYST, V142, P213, DOI 10.1080/11263500802150316; Srivastava AK, 2009, ANN BOT-LONDON, V103, P403, DOI 10.1093/aob/mcn229; Taji T, 2004, PLANT PHYSIOL, V135, P1697, DOI 10.1104/pp.104.039909; Touchette BW, 2007, J EXP MAR BIOL ECOL, V350, P194, DOI 10.1016/j.jembe.2007.05.037; Tyerman S.D., 1989, BIOL SEAGRASSES TREA, P723; vanWijk KJ, 1997, BIOCHEMISTRY-US, V36, P6178, DOI 10.1021/bi962921l; Wang LX, 2015, PLANT SCI, V231, P159, DOI 10.1016/j.plantsci.2014.11.013; Wang W, 2006, ELECTROPHORESIS, V27, P2782, DOI 10.1002/elps.200500722; Wilm M, 1996, NATURE, V379, P466, DOI 10.1038/379466a0; Wissler L, 2009, DATABASE-OXFORD, DOI 10.1093/database/bap009; Zhang LX, 2002, FEBS LETT, V512, P13, DOI 10.1016/S0014-5793(02)02218-4; Zhang Y, 2010, ANAL CHEM, V82, P2272, DOI 10.1021/ac9023999; ZIMMERMANN U, 1978, ANNU REV PLANT PHYS, V29, P121, DOI 10.1146/annurev.pp.29.060178.001005; Zybailov B, 2006, J PROTEOME RES, V5, P2339, DOI 10.1021/pr060161n
Cited Reference Count 56
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 7
Publisher City LAUSANNE
Publisher Address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date JUN 26
Year Published 2015
Volume 6
Article Number 464
Digital Object Identifier (DOI) 10.3389/fpls.2015.00464
Page Count 12
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number CN4RD
Unique Article Identifier WOS:000358417000001
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed