Publication Type | J |
Authors | Piro, A; Marin-Guirao, L; Serra, IA; Spadafora, A; Sandoval-Gil, JM; Bemardeau-Esteller, J; Femandez, JMR; Mazzuca, S |
Author Full Name | Piro, Amalia; Marin-Guirao, Lazaro; Serra, Ilia A.; Spadafora, Antonia; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.; Mazzuca, Silvia |
Title | The modulation of leaf metabolism plays a role in salt tolerance of Cymodocea nodosa exposed to hypersaline stress in mesocosms |
Source | FRONTIERS IN PLANT SCIENCE |
Language | English |
Document Type | Article |
Author Keywords | seagrasses; leaf proteomics; hypersaline; mesocosm |
Keywords Plus | SEAGRASS POSIDONIA-OCEANICA; REACTION-CENTER-II; PHOTOSYSTEM-II; D1 PROTEIN; SALINITY FLUCTUATION; PROTEOMIC ANALYSIS; MASS-SPECTROMETRY; ATPASE ACTIVITY; LIGHT; PLANT |
Abstract | Applying proteomics, we tested the physiological responses of the euryhaline seagrass Cymodocea nodosa to deliberate manipulation of salinity in a mesocosm system. Plants were subjected to a chronic hypersaline condition (43 psu) to compare protein expression and plant photochemistry responses after 15 and 30 days of exposure with those of plants cultured under normal/ambient saline conditions (37 psu). Results showed a general decline in the expression level of leaf proteins in hypersaline stressed plants, with more intense reductions after long-lasting exposure. Specifically, the carbon-fixing enzyme RuBisCo displayed a lower accumulation level in stressed plants relative to controls. In contrast, the key enzymes involved in the regulation of glycolysis, cytosolic glyceraldehyde-3-phosphate dehydrogenase, enolase 2 and triose-phosphate isomerase, showed significantly higher accumulation levels. These responses suggested a shift in carbon metabolism in stressed plants. Hypersaline stress also induced a significant alteration of the photosynthetic physiology of C. nodosa by means of a downregulation in structural proteins and enzymes of both PSII and PSI. However we found an over-expression of the cytochrome b559 alpha subunit of the PSII initial complex, which is a receptor for the PSII core proteins involved in biogenesis or repair processes and therefore potentially involved in the absence of effects at the photochemical level of stressed plants. As expected hypersalinity also affects vacuolar metabolism by increasing the leaf cell turgor pressure and enhancing the up-take of Na+ by overaccumulating the tonoplast specific intrinsic protein pyrophosphate-energized inorganic pyrophosphatase (H(+)-PPase) coupled to the Na+/H+-antiporter. The modulation of carbon metabolism and the enhancement of vacuole capacity in Na+ sequestration and osmolarity changes are discussed in relation to salt tolerance of C. nodosa. |
Author Address | [Piro, Amalia; Serra, Ilia A.; Spadafora, Antonia; Mazzuca, Silvia] Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, I-87036 Arcavacata Di Rende, Italy; [Marin-Guirao, Lazaro; Sandoval-Gil, Jose M.; Bemardeau-Esteller, Jaime; Femandez, Juan M. R.] Spanish Inst Oceanog, Oceanog Ctr Murcia, Murcia, Spain |
Reprint Address | Mazzuca, S (reprint author), Univ Calabria, Dipartimento Chim & Tecnol Chim, Lab Biol & Prote Vegetale, Ponte Bucci 12C, I-87036 Arcavacata Di Rende, Italy. |
E-mail Address | silvia.mazzuca@unical.it |
ResearcherID Number | MarAn-Guirao, LAzaro/A-3481-2013 |
ORCID Number | MarAn-Guirao, LAzaro/0000-0001-6240-8018; Mazzuca, Silvia/0000-0003-4648-2271; PIRO, Amalia/0000-0002-6296-621X |
Funding Agency and Grant Number | COST Action |
Funding Text | The authors are grateful to the COST Action E50906 |
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Cited Reference Count | 56 |
Times Cited | 7 |
Total Times Cited Count (WoS, BCI, and CSCD) | 7 |
Publisher | FRONTIERS RESEARCH FOUNDATION |
Publisher City | LAUSANNE |
Publisher Address | PO BOX 110, LAUSANNE, 1015, SWITZERLAND |
ISSN | 1664-462X |
29-Character Source Abbreviation | FRONT PLANT SCI |
ISO Source Abbreviation | Front. Plant Sci. |
Publication Date | JUN 26 |
Year Published | 2015 |
Volume | 6 |
Article Number | 464 |
Digital Object Identifier (DOI) | 10.3389/fpls.2015.00464 |
Page Count | 12 |
Web of Science Category | Plant Sciences |
Subject Category | Plant Sciences |
Document Delivery Number | CN4RD |
Unique Article Identifier | WOS:000358417000001 |
Plants associated with this reference |