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Publication Type J
Authors Gashgari, R; Gherbawy, Y; Ameen, F; Alsharari, S
Author Full Name Gashgari, Rukaia; Gherbawy, Youssuf; Ameen, Fuad; Alsharari, Salam
Title Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic Fungi From Medicinal Plants in Saudi Arabia
Source JUNDISHAPUR JOURNAL OF MICROBIOLOGY
Language English
Document Type Article
Author Keywords Endophytic Fungi; Medicinal Plants; Antimicrobial Activity
Keywords Plus ANTIFUNGAL ACTIVITIES; AQUILARIA-SINENSIS; SALT-MARSH; ANTITUMOR; LEAVES; BIODIVERSITY; DIVERSITY; CANADA
Abstract Background: Endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. Objectives: The current study aimed to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from medicinal plants in Saudi Arabia. Materials and Methods: Fungi growing on plant segments were isolated and identified based on morphological and molecular characteristics. The isolates were grouped into 35 distinct operational taxonomic units, based on the sequence of the internal transcribed spacer regions in the rRNA gene. The colonization frequency and the dominant fungi percentage of these endophytic fungi were calculated. A dual culture technique was adopted to investigate the antifungal activity of these endophytes. Results: Tamarix nilotica showed the highest endophytic diversity with a relative frequency of 27.27%, followed by Cressa cretica with a relative frequency of 19.27%. The most frequently isolated species was Penicillium chrysogenum with an overall colonization rate of 98.57%. Seven out of 35 endophytic fungi exhibited strong antifungal activity to all plant fungal pathogens tested. P. chrysogenum, Fusarium oxysporum, and F. nygamai exhibited the highest inhibition against the human pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Aspergillus sydowii, P. chrysogenum, and Eupenicillium crustaceum showed strong antimicrobial activity against Enterococcus faecalis. Conclusions: The antimicrobial activity of these endophytic microorganisms could be exploited in biotechnology, medicine, and agriculture.
Author Address [Gashgari, Rukaia] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia; [Gherbawy, Youssuf] Taif Univ, Dept Biol, At Taif, Saudi Arabia; [Ameen, Fuad] King Saud Univ, Fac Sci, Dept Bot & Microbiol, Riyadh, Saudi Arabia; [Alsharari, Salam] Al Gouf Univ, Dept Bot, Fac Sci, Al Gouf, Saudi Arabia
Reprint Address Ameen, F (reprint author), King Saud Univ, Fac Sci, Dept Bot & Microbiol, Riyadh, Saudi Arabia.
E-mail Address alfouad2004@hotmail.com
ResearcherID Number Fac Sci, KAU, Biol Sci Dept/L-4228-2013; Faculty of, Sciences, KAU/E-7305-2017; Ameen, Fuad/N-8881-2017
ORCID Number Ameen, Fuad/0000-0002-5185-022X
Funding Agency and Grant Number King Saud university, deanship of scientific research, college of science research center
Funding Text This work was supported by King Saud university, deanship of scientific research, college of science research center.
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Cited Reference Count 36
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher AHVAZ JUNDISHAPUR UNIV MED SCI
Publisher City AHVAZ
Publisher Address PO BOX 6357-33118, AHVAZ, 00000, IRAN
ISSN 2008-3645
29-Character Source Abbreviation JUNDISHAPUR J MICROB
ISO Source Abbreviation Jundishapur J. Microbiol.
Publication Date JAN
Year Published 2016
Volume 9
Issue 1
Article Number e26157
Digital Object Identifier (DOI) 10.5812/jjm.26157
Page Count 8
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number DJ0XB
Unique Article Identifier WOS:000373926600003
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