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Publication Type J
Authors Baisakh, N., P. K. Subudhi and N. P. Parami
Title cDNA-AFLP analysis reveals differential gene expression in response to salt stress in a halophyte Spartina alterniflora Loisel
Source Plant Science
Author Keywords abiotic stress; cDNA-AFLP; salinity; smooth cordgrass; transcriptional profiling membrane h+-atpase; genome-wide expression; salinity stress; cold stress; tolerance; arabidopsis; plant; proteins; drought; nacl
Abstract Salinity impedes the growth and productivity of various crop species. Genome-wide expression analysis has identified a number of salinity induced up- and/or down-regulated genes that are also common during dehydration and temperature stress in the glycophyte like Arabidopsis. To date, Spartina alterniflora L. (smooth cordgrass) has not been exploited to understand the molecular basis of its unusually high level of salt tolerance. We used cDNA-AFLP as an RNA imaging technique to identify transcripts that are strongly accumulated and are induced de novo in response to salinity stress. Among 213 isolated, we have identified 28 cDNAs that belong to different groups of genes involved in ion transport and compartmentalization, cell division, metabolism, and protein synthesis, etc. At different stages of salt stress the expression patterns of 14 such genes through RT-PCR were analyzed, and their direct and indirect relationship with salinity tolerance mechanism is discussed. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Author Address Louisiana State Univ, Ctr Agr, Dept Agron & Environm Management, Baton Rouge, LA 70803 USA. Subudhi, PK, Louisiana State Univ, Ctr Agr, Dept Agron & Environm Management, 104 MB Sturgis Hall, Baton Rouge, LA 70803 USA. psubudhi@agctr.lsu.edu
29-Character Source Abbreviation Plant Sci.
Publication Date Jun
Year Published 2006
Volume 170
Issue 6
Beginning Page 1141-1149
Unique Article Identifier ISI:000237498700015
Plants associated with this reference

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