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Publication Type J
Authors Luo, Y., R. Reid, D. Freese, C. B. Li, J. Watkins, H. Z. Shi, H. Y. Zhang, A. Loraine and B. H. Song
Title Salt tolerance response revealed by RNA-Seq in a diploid halophytic wild relative of sweet potato
Source Scientific Reports
Author Keywords arabidopsis-thaliana salinity tolerance stress tolerance na+/h+-antiport ion homeostasis abiotic stress plant inositol drought mechanisms
Abstract Crop wild relatives harbor exotic and novel genetic resources, which hold great potential for crop improvement. Ipomoea imperati is a wild diploid relative of sweet potato with the capability of high salinity tolerance. We compared the transcriptomes of I. imperati under salt stress vs. control to identify candidate genes and pathways involved in salt response. De novo assembly produced 67,911 transcripts with a high depth of coverage. A total of 39,902 putative genes were assigned annotations, and 936 and 220 genes involved in salt response in roots and leaves, respectively. Functional analysis indicated a whole system response during salt stress in I. imperati, which included four metabolic processes: sensory initiation, transcriptional reprogramming, cellular protein component change, and cellular homeostasis regulation. We identified a number of candidate genes involved in the ABA signaling pathway, as well as transcription factors, transporters, antioxidant enzymes, and enzymes associated with metabolism of synthesis and catalysis. Furthermore, two membrane transporter genes, including vacuole cation/proton exchanger and inositol transporter, were considered to play important roles in salt tolerance. This study provided valuable information not only for understanding the genetic basis of ecological adaptation but also for future application in sweet potato and other crop improvements.
Author Address [Luo, Yan; Freese, Daniella; Zhang, Hengyou; Song, Bao-Hua] Univ North Carolina Charlotte, Dept Biol Sci, Charlotte, NC 28223 USA. [Luo, Yan] Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China. [Reid, Robert; Freese, Daniella; Loraine, Ann] Univ North Carolina Charlotte, Dept Bioinformat & Genom, Charlotte, NC 28223 USA. [Li, Changbao] Monsanto Co, Breeding Grp, St Louis, MO 63141 USA. [Watkins, Jonathan] Univ North Carolina Charlotte, Dept Geog & Earth Sci, Charlotte, NC 28223 USA. [Shi, Huazhong] Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA. Song, BH (reprint author), Univ North Carolina Charlotte, Dept Biol Sci, Charlotte, NC 28223 USA.; Loraine, A (reprint author), Univ North Carolina Charlotte, Dept Bioinformat & Genom, Charlotte, NC 28223 USA. aloraine@uncc.edu; bsong5@uncc.edu
ISSN 2045-2322
ISBN 2045-2322
29-Character Source Abbreviation Sci Rep
Publication Date Aug
Year Published 2017
Volume 7
Digital Object Identifier (DOI) 10.1038/s41598-017-09241-x
Unique Article Identifier WOS:000408535300008
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