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Publication Type J
Authors Aman, S., N. ul Haq, S. Ahmed and S. N. Shakeel
Title Identifications and Validations of Reference Genes for Gene Expression Data Normalization of Chenopodium album
Source International Journal of Agriculture and Biology
Author Keywords Validation Internal controls Abiotic stress Chenopodium album time rt-pcr appropriate reference genes transcription-quantitative-pcr real-time housekeeping genes internal control abiotic stress molecular characterization selection rice
Abstract Quantitative real-time PCR (qRT-PCR) is considered as an important technique for gene expression and validation analysis for several high-throughput proteomics and genomics data. However, reliable qRT-PCR results are dependent on appropriate reference genes used for data normalization under specific experimental condition and such information is limited in case of plants whose genome is not sequenced yet. Therefore, we sequenced and evaluated stability of seven novel internal control genes (Act, Act alpha, beta-tub, 18SrRNA, GAPDH, EF1 alpha and Ubq) of Chenopodium album, an important edible plant with medicinal values and have been recently used in synthesis as well as analysis of several gold and silver nanoparticles. Statistical algorithms like geNorm and NormFinder were used and compared for the samples treated with different types of stresses including, high/low temperatures, drought, heavy metal and salt stress. beta-tubulin was revealed as the most stable genes under heat, drought and metal stress. While, 18SrRNA has shown the stable expression only under cold and salt stresses. Our data support the use of the combinations of two stable internal control genes for normalization of the gene expression. We compared the relative expression of a stress induced Chloroplast small Heat Shock Protein (Cp-sHSP) of C. album to further support our results. Together with all novel internal controls genes, this study also provides a platform for future gene expression studies of C. album using validated housekeeping genes. (C) 2017 Friends Science Publishers
Author Address [Aman, Sitwat; ul Haq, Noor; Shakeel, Samina N.] Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad 45320, Pakistan. [Aman, Sitwat] Univ Lahore, Inst Mol Biol & Biotechnol, Lahore 54590, Pakistan. [ul Haq, Noor] Khushal Khan Khattak Univ, Dept Comp Sci & BioInformat, Karak 27200, Khyber Pakhtunk, Pakistan. [Ahmed, Shakeel] Pakistan Inst Nucl Sci & Technol, Phys Div, Islamabad, Pakistan. [Shakeel, Samina N.] Dartmouth Coll, Fac Life Sci, Dept Biol, Hanover, NH 03755 USA. Shakeel, SN (reprint author), Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad 45320, Pakistan.; Shakeel, SN (reprint author), Dartmouth Coll, Fac Life Sci, Dept Biol, Hanover, NH 03755 USA. snq28@yahoo.com
ISSN 1560-8530
ISBN 1560-8530
29-Character Source Abbreviation Int. J. Agric. Biol.
Year Published 2017
Volume 19
Issue 4
Beginning Page 761-770
Digital Object Identifier (DOI) 10.17957/ijab/15.0354
Unique Article Identifier WOS:000408942100024

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