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Publication Type J
Authors Brandrud, MK; Paun, O; Lorenzo, MT; Nordal, I; Brysting, AK
Author Full Name Brandrud, Marie K.; Paun, Ovidiu; Lorenzo, Maria T.; Nordal, Inger; Brysting, Anne K.
Title RADseq provides evidence for parallel ecotypic divergence in the autotetraphoidCochlearia officinalis in Northern Norway
Source SCIENTIFIC REPORTS
Language English
Document Type Article
Keywords Plus MULTILOCUS GENOTYPE DATA; GAMMA-AMINOBUTYRIC-ACID; POPULATION-STRUCTURE; COCHLEARIA BRASSICACEAE; LOCAL ADAPTATION; TOOL SET; SPECIATION; EVOLUTION; GENOMICS; MARKERS
Abstract Speciation encompasses a continuum over time from freely interbreeding populations to reproductively isolated species. Along this process, ecotypes - the result of local adaptation - may be on the road to new species. We investigated whether three autotetraploid Cochlearia officinalis ecotypes, adapted to different habitats (beach, estuary, spring), are genetically differentiated and result from parallel ecotypic divergence in two distinct geographical regions. We obtained genetic data from thousands of single nucleotide polymorphisms (SNPs) from restriction-site associated DNA sequencing (RADseq) and from six microsatellite markers for 12 populations to assess genetic divergence at ecotypic, geographic and population level. The genetic patterns support differentiation among ecotypes as suggested by morphology and ecology. The data fit a scenario where the ancestral beach ecotype has recurrently and polytopically given rise to the estuary and spring ecotypes. Several ecologically-relevant loci with consistent non-random segregating patterns are identified across the recurrent origins, in particular around genes related to salt stress. Despite being ecologically distinct, the Cochlearia ecotypes still represent an early stage in the process of speciation, as reproductive isolation has not (yet) developed. A sequenced annotated genome is needed to specifically target candidate genes underlying local adaptation.
Author Address [Brandrud, Marie K.; Brysting, Anne K.] Univ Oslo, Dept Biosci, Ctr Ecol & Evolutionary Synth, N-0316 Oslo, Norway; [Brandrud, Marie K.; Paun, Ovidiu; Lorenzo, Maria T.] Univ Vienna, Dept Bot & Biodivers Res, A-1030 Vienna, Austria; [Nordal, Inger] Univ Oslo, Dept Biosci, N-0316 Oslo, Norway
Reprint Address Brandrud, MK (reprint author), Univ Oslo, Dept Biosci, Ctr Ecol & Evolutionary Synth, N-0316 Oslo, Norway.; Brandrud, MK (reprint author), Univ Vienna, Dept Bot & Biodivers Res, A-1030 Vienna, Austria.
E-mail Address marie.kristine.brandrud@univie.ac.at
Funding Agency and Grant Number Nansen Foundation, Systematics Research Fund; S.G.Sonneland Foundation; Austrian Science Fund project [FWF Y661-B16]
Funding Text We thank Pavel Travnicek for running the flow cytometry analyses, Anna Mazzarella and Emiliano Trucchi for help with initial STACKS analyses and python scripts, and Charlotte Bjora and Odd Stabbetorp for discussions on Cochlearia. The project was supported by the Nansen Foundation, Systematics Research Fund, S.G.Sonneland Foundation, and Professor Rathke, Professor Collett and Professor Wille's Legacy. M.K.B., M.T.L and O.P were in part funded through an Austrian Science Fund project (FWF Y661-B16).
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Cited Reference Count 88
Publisher NATURE PUBLISHING GROUP
Publisher City LONDON
Publisher Address MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
ISSN 2045-2322
29-Character Source Abbreviation SCI REP-UK
ISO Source Abbreviation Sci Rep
Publication Date JUL 17
Year Published 2017
Volume 7
Article Number 5573
Digital Object Identifier (DOI) 10.1038/s41598-017-05794-z
Page Count 13
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number FA8DC
Unique Article Identifier WOS:000405675400053
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